Human Gene CARD18 (ENST00000530950.2_7) from GENCODE V47lift37
  Description: caspase recruitment domain family member 18 (from RefSeq NM_021571.4)
Gencode Transcript: ENST00000530950.2_7
Gencode Gene: ENSG00000255501.3_10
Transcript (Including UTRs)
   Position: hg19 chr11:105,008,441-105,010,496 Size: 2,056 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg19 chr11:105,009,540-105,010,453 Size: 914 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:105,008,441-105,010,496)mRNA (may differ from genome)Protein (90 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CAR18_HUMAN
DESCRIPTION: RecName: Full=Caspase recruitment domain-containing protein 18; AltName: Full=Caspase-1 inhibitor Iceberg;
FUNCTION: Inhibits generation of IL-1-beta by interacting with caspase-1 and preventing its association with RIP2. Down-regulates the release of IL1B.
SUBUNIT: Interacts with pro-CASP1. Interacts with CARD8.
TISSUE SPECIFICITY: Primarily expressed in the heart and placenta.
INDUCTION: Up-regulated in response to TNF and bacterial lipopolysaccharides (LPS).
SIMILARITY: Contains 1 CARD domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.79 RPKM in Skin - Not Sun Exposed (Suprapubic)
Total median expression: 21.45 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -9.9043-0.230 Picture PostScript Text
3' UTR -85.80386-0.222 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001315 - CARD
IPR011029 - DEATH-like

Pfam Domains:
PF00619 - Caspase recruitment domain

SCOP Domains:
47986 - DEATH domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1DGN - NMR MuPIT


ModBase Predicted Comparative 3D Structure on P57730
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004869 cysteine-type endopeptidase inhibitor activity
GO:0005515 protein binding
GO:0030414 peptidase inhibitor activity
GO:0050700 CARD domain binding
GO:0089720 caspase binding
GO:0097200 cysteine-type endopeptidase activity involved in execution phase of apoptosis

Biological Process:
GO:0006954 inflammatory response
GO:0010466 negative regulation of peptidase activity
GO:0032091 negative regulation of protein binding
GO:0042981 regulation of apoptotic process
GO:0050713 negative regulation of interleukin-1 beta secretion
GO:0097194 execution phase of apoptosis
GO:0097340 inhibition of cysteine-type endopeptidase activity

Cellular Component:
GO:0005737 cytoplasm
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  AY358231 - Homo sapiens clone DNA101367 ICEBERG (UNQ5804) mRNA, complete cds.
BC131610 - Homo sapiens caspase recruitment domain family, member 18, mRNA (cDNA clone MGC:149416 IMAGE:40115072), complete cds.
AF208005 - Homo sapiens ICEBERG caspase-1 inhibitor (ICEBERG) mRNA, complete cds.
AB527895 - Synthetic construct DNA, clone: pF1KB0903, Homo sapiens CARD18 gene for caspase recruitment domain family, member 18, without stop codon, in Flexi system.

-  Other Names for This Gene
  Alternate Gene Symbols: A2RRF8, CAR18_HUMAN, ENST00000530950.1, ICEBERG, NM_021571, P57730, uc324icw.1, uc324icw.2, UNQ5804/PRO19611
UCSC ID: ENST00000530950.2_7
RefSeq Accession: NM_021571.4
Protein: P57730 (aka CAR18_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.