Human Gene CCL3 (ENST00000613922.2_7) from GENCODE V47lift37
  Description: C-C motif chemokine ligand 3, transcript variant 1 (from RefSeq NM_002983.3)
Gencode Transcript: ENST00000613922.2_7
Gencode Gene: ENSG00000277632.2_9
Transcript (Including UTRs)
   Position: hg19 chr17:34,415,602-34,417,489 Size: 1,888 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg19 chr17:34,416,018-34,417,404 Size: 1,387 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:34,415,602-34,417,489)mRNA (may differ from genome)Protein (92 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CCL3_HUMAN
DESCRIPTION: RecName: Full=C-C motif chemokine 3; AltName: Full=G0/G1 switch regulatory protein 19-1; AltName: Full=Macrophage inflammatory protein 1-alpha; Short=MIP-1-alpha; AltName: Full=PAT 464.1; AltName: Full=SIS-beta; AltName: Full=Small-inducible cytokine A3; AltName: Full=Tonsillar lymphocyte LD78 alpha protein; Contains: RecName: Full=MIP-1-alpha(4-69); AltName: Full=LD78-alpha(4-69); Flags: Precursor;
FUNCTION: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
SUBUNIT: Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69).
SUBCELLULAR LOCATION: Secreted.
INDUCTION: By TPA or PHA (TPA = 12-O-tetradecanoyl phorbol-13 acetate (tumor promoter); PHA = phytohemagglutinin (T-cell mitogen)).
PTM: N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T- cells.
SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
WEB RESOURCE: Name=Wikipedia; Note=Macrophage inflammatory protein entry; URL="http://en.wikipedia.org/wiki/Macrophage_Inflammatory_Protein";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CCL3
Diseases sorted by gene-association score: hiv-1* (18), rheumatoid arthritis (5), pneumonia (5), bronchiolitis (4), asthma (4), pulmonary tuberculosis (3), acquired immunodeficiency syndrome (3), lung disease (3), whiplash (3), demyelinating disease (3), schistosomiasis (3), trypanosomiasis (3), viral infectious disease (2), orofacial granulomatosis (2), stachybotrys chartarum (2), sarcoidosis 1 (2), malaria (2), tuberculous meningitis (2), pulmonary fibrosis, idiopathic (2), multiple myeloma (2), stromal keratitis (2), variola major (2), respiratory syncytial virus infectious disease (2), c6 deficiency (2), aplastic anemia (2), multiple sclerosis, disease progression, modifier of (2), autoinflammation, lipodystrophy, and dermatosis syndrome (2), human immunodeficiency virus infectious disease (1), necatoriasis (1), behcet syndrome (1), chronic nk-cell lymphocytosis (1)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -20.2085-0.238 Picture PostScript Text
3' UTR -134.90416-0.324 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000827 - Chemokine_CC_CS
IPR001811 - Chemokine_IL8-like_dom

Pfam Domains:
PF00048 - Small cytokines (intecrine/chemokine), interleukin-8 like

SCOP Domains:
54117 - Interleukin 8-like chemokines

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1B50 - NMR MuPIT 1B53 - NMR MuPIT 2X69 - X-ray 2X6G - X-ray MuPIT 3FPU - X-ray MuPIT 3H44 - X-ray MuPIT 3KBX - X-ray MuPIT 3TN1 - X-ray


ModBase Predicted Comparative 3D Structure on P10147
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004672 protein kinase activity
GO:0004698 calcium-dependent protein kinase C activity
GO:0005125 cytokine activity
GO:0005515 protein binding
GO:0008009 chemokine activity
GO:0016004 phospholipase activator activity
GO:0016301 kinase activity
GO:0031726 CCR1 chemokine receptor binding
GO:0031730 CCR5 chemokine receptor binding
GO:0042056 chemoattractant activity
GO:0042802 identical protein binding

Biological Process:
GO:0000165 MAPK cascade
GO:0001649 osteoblast differentiation
GO:0001775 cell activation
GO:0002548 monocyte chemotaxis
GO:0006816 calcium ion transport
GO:0006874 cellular calcium ion homeostasis
GO:0006887 exocytosis
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007010 cytoskeleton organization
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007267 cell-cell signaling
GO:0008360 regulation of cell shape
GO:0009636 response to toxic substance
GO:0010469 regulation of receptor activity
GO:0010628 positive regulation of gene expression
GO:0010629 negative regulation of gene expression
GO:0010818 T cell chemotaxis
GO:0014808 release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
GO:0019221 cytokine-mediated signaling pathway
GO:0019722 calcium-mediated signaling
GO:0023052 signaling
GO:0030335 positive regulation of cell migration
GO:0030502 negative regulation of bone mineralization
GO:0030593 neutrophil chemotaxis
GO:0031663 lipopolysaccharide-mediated signaling pathway
GO:0032760 positive regulation of tumor necrosis factor production
GO:0043308 eosinophil degranulation
GO:0043491 protein kinase B signaling
GO:0043525 positive regulation of neuron apoptotic process
GO:0043547 positive regulation of GTPase activity
GO:0043615 astrocyte cell migration
GO:0043922 negative regulation by host of viral transcription
GO:0045671 negative regulation of osteoclast differentiation
GO:0048245 eosinophil chemotaxis
GO:0048246 macrophage chemotaxis
GO:0048247 lymphocyte chemotaxis
GO:0050718 positive regulation of interleukin-1 beta secretion
GO:0050729 positive regulation of inflammatory response
GO:0050795 regulation of behavior
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050918 positive chemotaxis
GO:0051897 positive regulation of protein kinase B signaling
GO:0051928 positive regulation of calcium ion transport
GO:0051930 regulation of sensory perception of pain
GO:0070098 chemokine-mediated signaling pathway
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0070723 response to cholesterol
GO:0071346 cellular response to interferon-gamma
GO:0071347 cellular response to interleukin-1
GO:0071356 cellular response to tumor necrosis factor
GO:0071407 cellular response to organic cyclic compound
GO:0071621 granulocyte chemotaxis
GO:0090280 positive regulation of calcium ion import
GO:2000503 positive regulation of natural killer cell chemotaxis

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005622 intracellular
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  BC071834 - Homo sapiens chemokine (C-C motif) ligand 3, mRNA (cDNA clone MGC:88494 IMAGE:5935287), complete cds.
M25315 - Homo sapiens (clone pAT 464) potential lymphokine/cytokine mRNA, complete cds.
M23452 - Human macrophage inflammatory protein (G0S19-1) mRNA, complete cds.
D00044 - Homo sapiens mRNA for pLD78 peptide, complete cds.
X03754 - Human tonsillar lymphocyte LD78 mRNA induced by TPA or PHA TPA = 12-o-tetradecanoyl phorbol-13 acetate (tumor promoter) PHA = phytohemagglutinin (T-cell mitogen).
FW340036 - Screening.
JD084607 - Sequence 65631 from Patent EP1572962.
JD306608 - Sequence 287632 from Patent EP1572962.
JD393636 - Sequence 374660 from Patent EP1572962.
JD136480 - Sequence 117504 from Patent EP1572962.
JD554868 - Sequence 535892 from Patent EP1572962.
BC171831 - Homo sapiens chemokine (C-C motif) ligand 3, mRNA (cDNA clone MGC:198546 IMAGE:9054485), complete cds.
BC171891 - Homo sapiens chemokine (C-C motif) ligand 3, mRNA (cDNA clone MGC:198606 IMAGE:9054545), complete cds.
JD153988 - Sequence 135012 from Patent EP1572962.
JD210097 - Sequence 191121 from Patent EP1572962.
JD216426 - Sequence 197450 from Patent EP1572962.
E03130 - cDNA encoding human peptide LD78 alpha.
JD102727 - Sequence 83751 from Patent EP1572962.
JD130583 - Sequence 111607 from Patent EP1572962.
JD400565 - Sequence 381589 from Patent EP1572962.
AF043339 - Homo sapiens macrophage inflammatory protein 1 alpha (MIP1a) mRNA, partial cds.
KJ901725 - Synthetic construct Homo sapiens clone ccsbBroadEn_11119 CCL3 gene, encodes complete protein.
AB463487 - Synthetic construct DNA, clone: pF1KB6725, Homo sapiens CCL3 gene for chemokine (C-C motif) ligand 3, without stop codon, in Flexi system.
JD322676 - Sequence 303700 from Patent EP1572962.
JD402872 - Sequence 383896 from Patent EP1572962.
JD167198 - Sequence 148222 from Patent EP1572962.
JD469936 - Sequence 450960 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_nktPathway - Selective expression of chemokine receptors during T-cell polarization
h_erythPathway - Erythrocyte Differentiation Pathway

Reactome (by CSHL, EBI, and GO)

Protein P10147 (Reactome details) participates in the following event(s):

R-HSA-6783783 Interleukin-10 signaling
R-HSA-449147 Signaling by Interleukins
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: CCL3_HUMAN, ENST00000613922.1, G0S19-1, MIP1A, NM_002983, P10147, SCYA3, uc327oxe.1, uc327oxe.2
UCSC ID: ENST00000613922.2_7
RefSeq Accession: NM_002983.3
Protein: P10147 (aka CCL3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.