Human Gene CCL4L2 (ENST00000394465.2) from GENCODE V47lift37
  Description: Chemokine that induces chemotaxis of cells expressing CCR5 or CCR1. Inhibits HIV replication in peripheral blood monocytes that express CCR5. (from UniProt Q8NHW4)
Gencode Transcript: ENST00000394465.2
Gencode Gene: ENSG00000197262.8
Transcript (Including UTRs)
   Position: hg19 chr17:34,639,793-34,641,846 Size: 2,054 Total Exon Count: 3 Strand: +
Coding Region
   Position: hg19 chr17:34,640,110-34,641,537 Size: 1,428 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:34,639,793-34,641,846)mRNA (may differ from genome)Protein (92 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
EnsemblExonPrimerGeneCardsHGNCMGIPubMed
ReactomeUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: CC4L_HUMAN
DESCRIPTION: RecName: Full=C-C motif chemokine 4-like; AltName: Full=Lymphocyte activation gene 1 protein; Short=LAG-1; AltName: Full=Macrophage inflammatory protein 1-beta; Short=MIP-1-beta; AltName: Full=Monocyte adherence-induced protein 5-alpha; AltName: Full=Small-inducible cytokine A4-like; Flags: Precursor;
FUNCTION: Chemokine that induces chemotaxis of cells expressing CCR5 or CCR1. Inhibits HIV replication in peripheral blood monocytes that express CCR5.
SUBUNIT: Interacts with CCR5.
SUBCELLULAR LOCATION: Secreted (By similarity).
TISSUE SPECIFICITY: Detected in B-cells.
POLYMORPHISM: The copy number of the CC4L1 gene varies among individuals; most individuals have 1 to 6 copies in the diploid genome.
SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.75 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 32.07 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -79.80317-0.252 Picture PostScript Text
3' UTR -82.60309-0.267 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000827 - Chemokine_CC_CS
IPR001811 - Chemokine_IL8-like_dom

Pfam Domains:
PF00048 - Small cytokines (intecrine/chemokine), interleukin-8 like

SCOP Domains:
54117 - Interleukin 8-like chemokines

ModBase Predicted Comparative 3D Structure on Q8NHW4
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005125 cytokine activity
GO:0005515 protein binding
GO:0008009 chemokine activity
GO:0048020 CCR chemokine receptor binding

Biological Process:
GO:0002548 monocyte chemotaxis
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007186 G-protein coupled receptor signaling pathway
GO:0010469 regulation of receptor activity
GO:0030593 neutrophil chemotaxis
GO:0043547 positive regulation of GTPase activity
GO:0048247 lymphocyte chemotaxis
GO:0070098 chemokine-mediated signaling pathway
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0071346 cellular response to interferon-gamma
GO:0071347 cellular response to interleukin-1
GO:0071356 cellular response to tumor necrosis factor

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space


-  Descriptions from all associated GenBank mRNAs
  BC146944 - Homo sapiens chemokine (C-C motif) ligand 4-like 1, mRNA (cDNA clone MGC:182047 IMAGE:9056872), complete cds.
BC146958 - Homo sapiens chemokine (C-C motif) ligand 4-like 1, mRNA (cDNA clone MGC:182061 IMAGE:9056886), complete cds.
BC171857 - Homo sapiens similar to cytokine, mRNA (cDNA clone MGC:198572 IMAGE:9054511), complete cds.
BC171864 - Homo sapiens chemokine (C-C motif) ligand 4-like 1, mRNA (cDNA clone MGC:198579 IMAGE:9054518), complete cds.
AY766447 - Homo sapiens CC chemokine ligand 4L1 (CCL4L) mRNA, CCL4L-1 allele, complete cds, alternatively spliced.
AY766449 - Homo sapiens CC chemokine ligand 4L1d2 (CCL4L) mRNA, CCL4L-1 allele, complete cds, alternatively spliced.
AY766450 - Homo sapiens CC chemokine ligand 4L2 (CCL4L) mRNA, CCL4L-2 allele, complete cds, alternatively spliced.
AY766455 - Homo sapiens CC chemokine ligand 4L2e (CCL4L) mRNA, CCL4L-2 allele, complete cds, alternatively spliced.
AY766456 - Homo sapiens CC chemokine ligand 4L2d2 (CCL4L) mRNA, CCL4L-2 allele, complete cds, alternatively spliced.
BC070310 - Homo sapiens chemokine (C-C motif) ligand 4-like 2, mRNA (cDNA clone MGC:88312 IMAGE:6708338), complete cds.
JD260091 - Sequence 241115 from Patent EP1572962.
JD311045 - Sequence 292069 from Patent EP1572962.
BC071718 - Homo sapiens cDNA clone IMAGE:5204205, partial cds.
BC144394 - Homo sapiens chemokine (C-C motif) ligand 4-like 1, mRNA (cDNA clone MGC:177932 IMAGE:9052915), complete cds.
BC130456 - Homo sapiens chemokine (C-C motif) ligand 4-like 2, mRNA (cDNA clone MGC:163328 IMAGE:40146487), complete cds.
BC130458 - Homo sapiens chemokine (C-C motif) ligand 4-like 2, mRNA (cDNA clone MGC:163330 IMAGE:40146489), complete cds.
BC144393 - Homo sapiens chemokine (C-C motif) ligand 4-like 1, mRNA (cDNA clone MGC:177931 IMAGE:9052914), complete cds.
JD306887 - Sequence 287911 from Patent EP1572962.
HQ258694 - Synthetic construct Homo sapiens clone IMAGE:100072724 chemokine (C-C motif) ligand 4-like 2 (CCL4L2) gene, encodes complete protein.
KJ892804 - Synthetic construct Homo sapiens clone ccsbBroadEn_02198 CCL4L1 gene, encodes complete protein.
BC146445 - Synthetic construct Homo sapiens clone IMAGE:100015106, MGC:180204 chemokine (C-C motif) ligand 4-like 1 (CCL4L1) mRNA, encodes complete protein.
BC148784 - Synthetic construct Homo sapiens clone IMAGE:100015826, MGC:183187 chemokine (C-C motif) ligand 4-like 1 (CCL4L1) mRNA, encodes complete protein.
JD103635 - Sequence 84659 from Patent EP1572962.
JD251356 - Sequence 232380 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8NHW4 (Reactome details) participates in the following event(s):

R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-418594 G alpha (i) signalling events
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: AY766447, B2RUZ3, B7ZMA8, CC4L_HUMAN, CCL4L, CCL4L1, ENST00000394465.1, LAG1, Q50EM1, Q50EM2, Q50EM3, Q50EM4, Q50EM5, Q50EM6, Q50EM7, Q50EM8, Q569J2, Q6NSB0, Q8NHW4, SCYA4L1, SCYA4L2, uc318wrb.1
UCSC ID: ENST00000394465.2
RefSeq Accession: NM_000014.6
Protein: Q8NHW4 (aka CC4L_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.