Human Gene CDC34 (ENST00000215574.9_4) from GENCODE V47lift37
  Description: cell division cycle 34, ubiqiutin conjugating enzyme (from RefSeq NM_004359.2)
Gencode Transcript: ENST00000215574.9_4
Gencode Gene: ENSG00000099804.9_7
Transcript (Including UTRs)
   Position: hg19 chr19:531,760-542,087 Size: 10,328 Total Exon Count: 5 Strand: +
Coding Region
   Position: hg19 chr19:531,932-541,552 Size: 9,621 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:531,760-542,087)mRNA (may differ from genome)Protein (236 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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-  Comments and Description Text from UniProtKB
  ID: UB2R1_HUMAN
DESCRIPTION: RecName: Full=Ubiquitin-conjugating enzyme E2 R1; EC=6.3.2.19; AltName: Full=Ubiquitin-conjugating enzyme E2-32 kDa complementing; AltName: Full=Ubiquitin-conjugating enzyme E2-CDC34; AltName: Full=Ubiquitin-protein ligase R1;
FUNCTION: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.
CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
ENZYME REGULATION: CDC34-catalyzed polyubiquitin chain assembly activity is stimulated by the conjugation of NEDD8 to the CUL1 SCF E3 ligase complex subunit.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.11 uM for beta-catenin-monoubiquin;
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened. When phosphorylated, interacts with beta-TrCP (BTRC). Interacts with human herpes virus 1 protein ICP0 and associates with the proteasome for degradation. Interacts with casein kinase subunit CSNK2B.
INTERACTION: Q13616:CUL1; NbExp=3; IntAct=EBI-975634, EBI-359390;
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.
TISSUE SPECIFICITY: Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
INDUCTION: Negatively regulated by the let-7 microRNA.
DOMAIN: The C-terminal acidic tail is required for nuclear localization and is involved in the binding to SCF E3 ligase complexes, and more specifically with the CUL1 subunit.
PTM: Autoubiquitinated. Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway.
PTM: Phosphorylated by CK2. Phosphorylation of the C-terminal tail by CK2 controles the nuclear localization.
SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
SEQUENCE CAUTION: Sequence=AAC37534.1; Type=Erroneous initiation;
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/cdc34/";

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 82.80 RPKM in Testis
Total median expression: 1337.84 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -100.50172-0.584 Picture PostScript Text
3' UTR -225.50535-0.421 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000608 - UBQ-conjugat_E2
IPR023313 - UBQ-conjugating_AS
IPR016135 - UBQ-conjugating_enzyme/RWD

Pfam Domains:
PF00179 - Ubiquitin-conjugating enzyme

SCOP Domains:
54495 - UBC-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2OB4 - X-ray MuPIT 3RZ3 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P49427
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0031625 ubiquitin protein ligase binding
GO:0061630 ubiquitin protein ligase activity
GO:0061631 ubiquitin conjugating enzyme activity

Biological Process:
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000209 protein polyubiquitination
GO:0006270 DNA replication initiation
GO:0006464 cellular protein modification process
GO:0007049 cell cycle
GO:0016567 protein ubiquitination
GO:0035458 cellular response to interferon-beta
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0043525 positive regulation of neuron apoptotic process
GO:0043951 negative regulation of cAMP-mediated signaling
GO:0070848 response to growth factor
GO:0070936 protein K48-linked ubiquitination
GO:0090261 positive regulation of inclusion body assembly

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016607 nuclear speck


-  Descriptions from all associated GenBank mRNAs
  BC018143 - Homo sapiens cell division cycle 34 homolog (S. cerevisiae), mRNA (cDNA clone MGC:9581 IMAGE:3884179), complete cds.
AK291554 - Homo sapiens cDNA FLJ75711 complete cds, highly similar to Homo sapiens cell division cycle 34 (CDC34), mRNA.
BC009850 - Homo sapiens cell division cycle 34 homolog (S. cerevisiae), mRNA (cDNA clone MGC:15417 IMAGE:3942735), complete cds.
L22005 - Human ubiquitin conjugating enzyme mRNA, partial cds.
LF209263 - JP 2014500723-A/16766: Polycomb-Associated Non-Coding RNAs.
BC023979 - Homo sapiens cell division cycle 34 homolog (S. cerevisiae), mRNA (cDNA clone MGC:22659 IMAGE:4103120), complete cds.
JD465539 - Sequence 446563 from Patent EP1572962.
JD211253 - Sequence 192277 from Patent EP1572962.
JD471461 - Sequence 452485 from Patent EP1572962.
AB590273 - Synthetic construct DNA, clone: pFN21AE1471, Homo sapiens CDC34 gene for cell division cycle 34 homolog, without stop codon, in Flexi system.
DQ890738 - Synthetic construct clone IMAGE:100003368; FLH165580.01X; RZPDo839E02160D cell division cycle 34 (CDC34) gene, encodes complete protein.
DQ893910 - Synthetic construct Homo sapiens clone IMAGE:100008370; FLH165576.01L; RZPDo839E02159D cell division cycle 34 (CDC34) gene, encodes complete protein.
DQ894652 - Synthetic construct Homo sapiens clone IMAGE:100009112; FLH177239.01L; RZPDo839E12123D cell division cycle 34 (CDC34) gene, encodes complete protein.
CU676208 - Synthetic construct Homo sapiens gateway clone IMAGE:100016843 5' read CDC34 mRNA.
KJ890880 - Synthetic construct Homo sapiens clone ccsbBroadEn_00274 CDC34 gene, encodes complete protein.
BT006659 - Homo sapiens cell division cycle 34 mRNA, complete cds.
LF327308 - JP 2014500723-A/134811: Polycomb-Associated Non-Coding RNAs.
LF327309 - JP 2014500723-A/134812: Polycomb-Associated Non-Coding RNAs.
BC002332 - Homo sapiens cell division cycle 34 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:3536880), with apparent retained intron.
JD254994 - Sequence 236018 from Patent EP1572962.
LF327311 - JP 2014500723-A/134814: Polycomb-Associated Non-Coding RNAs.
JD543509 - Sequence 524533 from Patent EP1572962.
JD253473 - Sequence 234497 from Patent EP1572962.
JD214150 - Sequence 195174 from Patent EP1572962.
JD443986 - Sequence 425010 from Patent EP1572962.
JD136483 - Sequence 117507 from Patent EP1572962.
JD117874 - Sequence 98898 from Patent EP1572962.
JD200908 - Sequence 181932 from Patent EP1572962.
JD458204 - Sequence 439228 from Patent EP1572962.
JD476441 - Sequence 457465 from Patent EP1572962.
JD409956 - Sequence 390980 from Patent EP1572962.
JD555693 - Sequence 536717 from Patent EP1572962.
JD058858 - Sequence 39882 from Patent EP1572962.
JD239554 - Sequence 220578 from Patent EP1572962.
JD105806 - Sequence 86830 from Patent EP1572962.
JD194357 - Sequence 175381 from Patent EP1572962.
JD128060 - Sequence 109084 from Patent EP1572962.
JD484688 - Sequence 465712 from Patent EP1572962.
JD136409 - Sequence 117433 from Patent EP1572962.
JD487754 - Sequence 468778 from Patent EP1572962.
JD255440 - Sequence 236464 from Patent EP1572962.
JD255441 - Sequence 236465 from Patent EP1572962.
JD154563 - Sequence 135587 from Patent EP1572962.
JD297721 - Sequence 278745 from Patent EP1572962.
MA562885 - JP 2018138019-A/134811: Polycomb-Associated Non-Coding RNAs.
MA562886 - JP 2018138019-A/134812: Polycomb-Associated Non-Coding RNAs.
MA562888 - JP 2018138019-A/134814: Polycomb-Associated Non-Coding RNAs.
MA444840 - JP 2018138019-A/16766: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-7511 - protein ubiquitination

BioCarta from NCI Cancer Genome Anatomy Project
h_g2Pathway - Cell Cycle: G2/M Checkpoint
h_fbw7Pathway - Cyclin E Destruction Pathway
h_skp2e2fPathway - E2F1 Destruction Pathway

Reactome (by CSHL, EBI, and GO)

Protein P49427 (Reactome details) participates in the following event(s):

R-HSA-8852129 UBA1 conjugates ubiquitin to cytosolic E2 enzymes
R-HSA-983152 Transfer of ubiquitin from E1 to E2
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-8852130 UBA1 conjugates ubiquitin to nuclear E2 enzymes
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-202424 Downstream TCR signaling
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-8852135 Protein ubiquitination
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-202403 TCR signaling
R-HSA-2454202 Fc epsilon receptor (FCERI) signaling
R-HSA-5621481 C-type lectin receptors (CLRs)
R-HSA-597592 Post-translational protein modification
R-HSA-1280218 Adaptive Immune System
R-HSA-168249 Innate Immune System
R-HSA-392499 Metabolism of proteins
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A8K689, ENST00000215574.1, ENST00000215574.2, ENST00000215574.3, ENST00000215574.4, ENST00000215574.5, ENST00000215574.6, ENST00000215574.7, ENST00000215574.8, NM_004359, P49427, UB2R1_HUMAN, UBCH3, UBE2R1, uc317cmf.1, uc317cmf.2
UCSC ID: ENST00000215574.9_4
RefSeq Accession: NM_004359.2
Protein: P49427 (aka UB2R1_HUMAN or UBC3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.