Human Gene CMBL (ENST00000296658.4_7) from GENCODE V47lift37
  Description: carboxymethylenebutenolidase homolog (from RefSeq NM_138809.4)
Gencode Transcript: ENST00000296658.4_7
Gencode Gene: ENSG00000164237.9_9
Transcript (Including UTRs)
   Position: hg19 chr5:10,277,707-10,308,014 Size: 30,308 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg19 chr5:10,280,565-10,290,874 Size: 10,310 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:10,277,707-10,308,014)mRNA (may differ from genome)Protein (245 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CMBL_HUMAN
DESCRIPTION: RecName: Full=Carboxymethylenebutenolidase homolog; EC=3.1.-.-;
FUNCTION: Cysteine hydrolase. Can convert the prodrug olmesartan medoxomil into its pharmacologically active metabolite olmerstatan, an angiotensin receptor blocker, in liver and intestine. May also activate beta-lactam antibiotics faropenem medoxomil and lenampicillin.
ENZYME REGULATION: Strongly inhibited by p-chloromercuribenzoate (PCMB). Partially inhibited by bis-p-nitrophenylphosphate (BNPP). Not inhibited by DFP, PMSF, eserine or EDTA.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=170 uM for olmesartan medoxomil; KM=283 uM for faropenem medoxomil; KM=63.4 uM for lenampicillin; Vmax=24.6 nmol/min/mg enzyme toward olmesartan medoxomil; Vmax=16.4 nmol/min/mg enzyme toward faropenem medoxomil; Vmax=4 nmol/min/mg enzyme toward lenampicillin;
SUBCELLULAR LOCATION: Cytoplasm, cytosol.
TISSUE SPECIFICITY: Widely expressed, with highest levels in liver, followed by kidney, small intestine and colon. Present in liver and intestine (at protein level).
SIMILARITY: Belongs to the dienelactone hydrolase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 35.78 RPKM in Colon - Transverse
Total median expression: 461.36 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -88.60297-0.298 Picture PostScript Text
3' UTR -950.102858-0.332 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002925 - Dienelactn_hydro

Pfam Domains:
PF01738 - Dienelactone hydrolase family

SCOP Domains:
46689 - Homeodomain-like
53474 - alpha/beta-Hydrolases

ModBase Predicted Comparative 3D Structure on Q96DG6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene DetailsGene Details   Gene Details
Gene SorterGene Sorter   Gene Sorter
 RGDEnsembl  SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0016787 hydrolase activity

Biological Process:
GO:0006805 xenobiotic metabolic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AJ278125 - Homo sapiens mRNA for hypothetical protein (ORF1), clone Telethon(Italy_B41)_Strait00315_FL134.
BX647141 - Homo sapiens mRNA; cDNA DKFZp686H11239 (from clone DKFZp686H11239).
BC001573 - Homo sapiens carboxymethylenebutenolidase homolog (Pseudomonas), mRNA (cDNA clone MGC:5522 IMAGE:3454199), complete cds.
JD469739 - Sequence 450763 from Patent EP1572962.
JD517315 - Sequence 498339 from Patent EP1572962.
JD366228 - Sequence 347252 from Patent EP1572962.
JD461948 - Sequence 442972 from Patent EP1572962.
JD538044 - Sequence 519068 from Patent EP1572962.
JD344751 - Sequence 325775 from Patent EP1572962.
JD447299 - Sequence 428323 from Patent EP1572962.
JD139193 - Sequence 120217 from Patent EP1572962.
JD227488 - Sequence 208512 from Patent EP1572962.
JD170116 - Sequence 151140 from Patent EP1572962.
JD529629 - Sequence 510653 from Patent EP1572962.
JD421338 - Sequence 402362 from Patent EP1572962.
JD147524 - Sequence 128548 from Patent EP1572962.
JD047606 - Sequence 28630 from Patent EP1572962.
JD135792 - Sequence 116816 from Patent EP1572962.
JD165795 - Sequence 146819 from Patent EP1572962.
JD347808 - Sequence 328832 from Patent EP1572962.
JD258757 - Sequence 239781 from Patent EP1572962.
JD240988 - Sequence 222012 from Patent EP1572962.
JD105911 - Sequence 86935 from Patent EP1572962.
JD258756 - Sequence 239780 from Patent EP1572962.
JD101248 - Sequence 82272 from Patent EP1572962.
JD189661 - Sequence 170685 from Patent EP1572962.
JD269503 - Sequence 250527 from Patent EP1572962.
JD281936 - Sequence 262960 from Patent EP1572962.
JD361192 - Sequence 342216 from Patent EP1572962.
JD388350 - Sequence 369374 from Patent EP1572962.
JD321131 - Sequence 302155 from Patent EP1572962.
JD041386 - Sequence 22410 from Patent EP1572962.
JD169367 - Sequence 150391 from Patent EP1572962.
JD061824 - Sequence 42848 from Patent EP1572962.
JD506900 - Sequence 487924 from Patent EP1572962.
JD446690 - Sequence 427714 from Patent EP1572962.
JD348817 - Sequence 329841 from Patent EP1572962.
JD118446 - Sequence 99470 from Patent EP1572962.
JD313599 - Sequence 294623 from Patent EP1572962.
JD388349 - Sequence 369373 from Patent EP1572962.
JD512156 - Sequence 493180 from Patent EP1572962.
JD388348 - Sequence 369372 from Patent EP1572962.
JD169366 - Sequence 150390 from Patent EP1572962.
JD450697 - Sequence 431721 from Patent EP1572962.
JD063038 - Sequence 44062 from Patent EP1572962.
JD359935 - Sequence 340959 from Patent EP1572962.
JD404294 - Sequence 385318 from Patent EP1572962.
AK074197 - Homo sapiens cDNA FLJ23617 fis, clone ADKA03332.
JD179267 - Sequence 160291 from Patent EP1572962.
JD208538 - Sequence 189562 from Patent EP1572962.
JD481176 - Sequence 462200 from Patent EP1572962.
JD280825 - Sequence 261849 from Patent EP1572962.
JD384688 - Sequence 365712 from Patent EP1572962.
JD223484 - Sequence 204508 from Patent EP1572962.
JD420277 - Sequence 401301 from Patent EP1572962.
JD171473 - Sequence 152497 from Patent EP1572962.
AK315390 - Homo sapiens cDNA, FLJ96437.
CU674770 - Synthetic construct Homo sapiens gateway clone IMAGE:100018602 5' read CMBL mRNA.
HQ447384 - Synthetic construct Homo sapiens clone IMAGE:100070705; CCSB002422_02 carboxymethylenebutenolidase homolog (Pseudomonas) (CMBL) gene, encodes complete protein.
KJ895494 - Synthetic construct Homo sapiens clone ccsbBroadEn_04888 CMBL gene, encodes complete protein.
JD129522 - Sequence 110546 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q96DG6 (Reactome details) participates in the following event(s):

R-HSA-6786190 CMBL hydrolyses OM to OLMS
R-HSA-211945 Phase I - Functionalization of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: CMBL_HUMAN, D3DTC7, ENST00000296658.1, ENST00000296658.2, ENST00000296658.3, NM_138809, Q8TED6, Q96DG6, uc317lvs.1, uc317lvs.2
UCSC ID: ENST00000296658.4_7
RefSeq Accession: NM_138809.4
Protein: Q96DG6 (aka CMBL_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.