Human Gene CYB5B (ENST00000307892.13_7) from GENCODE V47lift37
  Description: cytochrome b5 type B (from RefSeq NM_030579.3)
Gencode Transcript: ENST00000307892.13_7
Gencode Gene: ENSG00000103018.18_15
Transcript (Including UTRs)
   Position: hg19 chr16:69,458,522-69,500,167 Size: 41,646 Total Exon Count: 5 Strand: +
Coding Region
   Position: hg19 chr16:69,458,587-69,496,423 Size: 37,837 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:69,458,522-69,500,167)mRNA (may differ from genome)Protein (150 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CYB5B_HUMAN
DESCRIPTION: RecName: Full=Cytochrome b5 type B; AltName: Full=Cytochrome b5 outer mitochondrial membrane isoform; Flags: Precursor;
FUNCTION: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases (By similarity).
SUBUNIT: Component of a complex composed of cytochrome b5, NADH- cytochrome b5 reductase (CYB5R3) and MOSC2 (By similarity).
SUBCELLULAR LOCATION: Mitochondrion outer membrane (By similarity).
SIMILARITY: Belongs to the cytochrome b5 family.
SIMILARITY: Contains 1 cytochrome b5 heme-binding domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 90.34 RPKM in Adrenal Gland
Total median expression: 710.83 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -15.1065-0.232 Picture PostScript Text
3' UTR -1020.003744-0.272 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR023271 - Aquaporin-like
IPR001199 - Cyt_B5
IPR018506 - Cyt_B5_heme-BS

Pfam Domains:
PF00173 - Cytochrome b5-like Heme/Steroid binding domain

SCOP Domains:
55856 - Cytochrome b5-like heme/steroid binding domain
81338 - Aquaporin-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3NER - X-ray


ModBase Predicted Comparative 3D Structure on O43169
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene DetailsGene Details   Gene Details
Gene SorterGene Sorter   Gene Sorter
 RGD   SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0020037 heme binding
GO:0046872 metal ion binding

Biological Process:
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005739 mitochondrion
GO:0005741 mitochondrial outer membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AB009282 - Homo sapiens mRNA for cytochrome b5, partial cds.
BX647922 - Homo sapiens mRNA; cDNA DKFZp686M0619 (from clone DKFZp686M0619).
BC014431 - Homo sapiens cytochrome b5 type B (outer mitochondrial membrane), mRNA (cDNA clone MGC:2040 IMAGE:3542294), complete cds.
BC004373 - Homo sapiens cytochrome b5 type B (outer mitochondrial membrane), mRNA (cDNA clone MGC:10477 IMAGE:3639133), complete cds.
AK291576 - Homo sapiens cDNA FLJ75270 complete cds, highly similar to Homo sapiens cytochrome b5 type B (outer mitochondrial membrane), mRNA.
JF432735 - Synthetic construct Homo sapiens clone IMAGE:100073978 cytochrome b5 type B (outer mitochondrial membrane) (CYB5B) gene, encodes complete protein.
KJ903311 - Synthetic construct Homo sapiens clone ccsbBroadEn_12705 CYB5B gene, encodes complete protein.
JD023637 - Sequence 4661 from Patent EP1572962.
JD028958 - Sequence 9982 from Patent EP1572962.
JD230464 - Sequence 211488 from Patent EP1572962.
JD391370 - Sequence 372394 from Patent EP1572962.
JD056944 - Sequence 37968 from Patent EP1572962.
JD224526 - Sequence 205550 from Patent EP1572962.
JD198858 - Sequence 179882 from Patent EP1572962.
JD438713 - Sequence 419737 from Patent EP1572962.
JD217902 - Sequence 198926 from Patent EP1572962.
JD146384 - Sequence 127408 from Patent EP1572962.
JD283373 - Sequence 264397 from Patent EP1572962.
JD213544 - Sequence 194568 from Patent EP1572962.
JD463526 - Sequence 444550 from Patent EP1572962.
JD147614 - Sequence 128638 from Patent EP1572962.
JD515192 - Sequence 496216 from Patent EP1572962.
JD471840 - Sequence 452864 from Patent EP1572962.
JD117093 - Sequence 98117 from Patent EP1572962.
JD156716 - Sequence 137740 from Patent EP1572962.
JD063374 - Sequence 44398 from Patent EP1572962.
JD172032 - Sequence 153056 from Patent EP1572962.
JD511860 - Sequence 492884 from Patent EP1572962.
JD474613 - Sequence 455637 from Patent EP1572962.
JD548629 - Sequence 529653 from Patent EP1572962.
JD494605 - Sequence 475629 from Patent EP1572962.
JD301937 - Sequence 282961 from Patent EP1572962.
JD043512 - Sequence 24536 from Patent EP1572962.
JD385674 - Sequence 366698 from Patent EP1572962.
JD488874 - Sequence 469898 from Patent EP1572962.
AK098148 - Homo sapiens cDNA FLJ40829 fis, clone TRACH2011894.
JD499181 - Sequence 480205 from Patent EP1572962.
JD313052 - Sequence 294076 from Patent EP1572962.
JD349431 - Sequence 330455 from Patent EP1572962.
JD559152 - Sequence 540176 from Patent EP1572962.
JD295820 - Sequence 276844 from Patent EP1572962.
JD398022 - Sequence 379046 from Patent EP1572962.
JD284532 - Sequence 265556 from Patent EP1572962.
JD433690 - Sequence 414714 from Patent EP1572962.
JD490126 - Sequence 471150 from Patent EP1572962.
JD284182 - Sequence 265206 from Patent EP1572962.
AK125218 - Homo sapiens cDNA FLJ43228 fis, clone HCHON2000418.
JD340510 - Sequence 321534 from Patent EP1572962.
JD047291 - Sequence 28315 from Patent EP1572962.
JD199245 - Sequence 180269 from Patent EP1572962.
JD102148 - Sequence 83172 from Patent EP1572962.
JD226183 - Sequence 207207 from Patent EP1572962.
JD040038 - Sequence 21062 from Patent EP1572962.
JD306506 - Sequence 287530 from Patent EP1572962.
JD334904 - Sequence 315928 from Patent EP1572962.
JD455702 - Sequence 436726 from Patent EP1572962.
JD173882 - Sequence 154906 from Patent EP1572962.
JD424097 - Sequence 405121 from Patent EP1572962.
JD507562 - Sequence 488586 from Patent EP1572962.
JD233701 - Sequence 214725 from Patent EP1572962.
JD294846 - Sequence 275870 from Patent EP1572962.
JD168279 - Sequence 149303 from Patent EP1572962.
JD250858 - Sequence 231882 from Patent EP1572962.
JD565922 - Sequence 546946 from Patent EP1572962.
JD450591 - Sequence 431615 from Patent EP1572962.
JD037788 - Sequence 18812 from Patent EP1572962.
JD299806 - Sequence 280830 from Patent EP1572962.
JD360832 - Sequence 341856 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O43169 (Reactome details) participates in the following event(s):

R-HSA-8936442 MARC1,MARC2 reduce N-hydroxylated compounds
R-HSA-211945 Phase I - Functionalization of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: A8K6B1, CYB5B_HUMAN, CYB5M, ENST00000307892.1, ENST00000307892.10, ENST00000307892.11, ENST00000307892.12, ENST00000307892.2, ENST00000307892.3, ENST00000307892.4, ENST00000307892.5, ENST00000307892.6, ENST00000307892.7, ENST00000307892.8, ENST00000307892.9, J3KNF8, NM_030579, O43169, OMB5, Q96CC3, Q9BT35, uc317oeh.1, uc317oeh.2
UCSC ID: ENST00000307892.13_7
RefSeq Accession: NM_030579.3
Protein: O43169 (aka CYB5B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.