Human Gene CYP4F3 (ENST00000221307.13_10) from GENCODE V47lift37
  Description: cytochrome P450 family 4 subfamily F member 3, transcript variant 1 (from RefSeq NM_000896.3)
Gencode Transcript: ENST00000221307.13_10
Gencode Gene: ENSG00000186529.16_15
Transcript (Including UTRs)
   Position: hg19 chr19:15,751,707-15,773,635 Size: 21,929 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg19 chr19:15,752,226-15,770,195 Size: 17,970 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:15,751,707-15,773,635)mRNA (may differ from genome)Protein (520 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CP4F3_HUMAN
DESCRIPTION: RecName: Full=Leukotriene-B(4) omega-hydroxylase 2; EC=1.14.13.30; AltName: Full=CYPIVF3; AltName: Full=Cytochrome P450 4F3; AltName: Full=Cytochrome P450-LTB-omega; AltName: Full=Leukotriene-B(4) 20-monooxygenase 2;
FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases. This enzyme requires molecular oxygen and NADPH for the omega-hydroxylation of LTB4, a potent chemoattractant for polymorphonuclear leukocytes.
CATALYTIC ACTIVITY: (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa- 6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)- 5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O.
COFACTOR: Heme group (By similarity).
ENZYME REGULATION: Inhibited by carbon monoxide (CO).
PATHWAY: Lipid metabolism; leukotriene B4 degradation.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity). Microsome membrane; Single-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Expressed in the polymorphonuclear leukocytes as well as leukocytes.
SIMILARITY: Belongs to the cytochrome P450 family.
SEQUENCE CAUTION: Sequence=AAC08589.1; Type=Frameshift; Positions=67, 114;
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/cyp4f3/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CYP4F3
Diseases sorted by gene-association score: inflammatory bowel disease 6 (12), ascariasis (7), colonic benign neoplasm (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 76.09 RPKM in Liver
Total median expression: 139.12 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -8.6050-0.172 Picture PostScript Text
3' UTR -1134.603440-0.330 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001128 - Cyt_P450
IPR017972 - Cyt_P450_CS
IPR002401 - Cyt_P450_E_grp-I

Pfam Domains:
PF00067 - Cytochrome P450

SCOP Domains:
48264 - Cytochrome P450

ModBase Predicted Comparative 3D Structure on Q08477
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0016491 oxidoreductase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0046872 metal ion binding
GO:0050051 leukotriene-B4 20-monooxygenase activity
GO:0052870 tocopherol omega-hydroxylase activity
GO:0052871 alpha-tocopherol omega-hydroxylase activity
GO:0097258 20-hydroxy-leukotriene B4 omega oxidase activity
GO:0097259 20-aldehyde-leukotriene B4 20-monooxygenase activity

Biological Process:
GO:0006629 lipid metabolic process
GO:0006690 icosanoid metabolic process
GO:0006691 leukotriene metabolic process
GO:0036101 leukotriene B4 catabolic process
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0031090 organelle membrane
GO:0043231 intracellular membrane-bounded organelle


-  Descriptions from all associated GenBank mRNAs
  AK316136 - Homo sapiens cDNA, FLJ79035 complete cds, highly similar to Cytochrome P450 4F2 (EC 1.14.13.30).
AK296802 - Homo sapiens cDNA FLJ50565 complete cds, highly similar to Cytochrome P450 4F3 (EC 1.14.13.30).
AK298675 - Homo sapiens cDNA FLJ56125 complete cds, highly similar to Cytochrome P450 4F3 (EC 1.14.13.30).
AK296761 - Homo sapiens cDNA FLJ56100 complete cds, highly similar to Cytochrome P450 4F2 (EC 1.14.13.30).
LF384394 - JP 2014500723-A/191897: Polycomb-Associated Non-Coding RNAs.
AB002454 - Homo sapiens mRNA for leukotriene B4 omega-hydroxylase, complete cds.
JD436020 - Sequence 417044 from Patent EP1572962.
JD042766 - Sequence 23790 from Patent EP1572962.
AK304200 - Homo sapiens cDNA FLJ52393 complete cds, highly similar to Cytochrome P450 4F2 (EC 1.14.13.30).
BC136299 - Homo sapiens cytochrome P450, family 4, subfamily F, polypeptide 3, mRNA (cDNA clone MGC:167909 IMAGE:9020286), complete cds.
D12620 - Homo sapiens mRNA for cytochrome P-450LTBV, complete cds.
JD233211 - Sequence 214235 from Patent EP1572962.
D12621 - Homo sapiens mRNA for cytochrome P-450LTBV, partial cds, clone:S-8.
AF054821 - Homo sapiens cytochrome P-450 mRNA, complete cds.
JD341276 - Sequence 322300 from Patent EP1572962.
AK303477 - Homo sapiens cDNA FLJ50432 complete cds, highly similar to Cytochrome P450 4F3 (EC 1.14.13.30).
JD439877 - Sequence 420901 from Patent EP1572962.
JD091802 - Sequence 72826 from Patent EP1572962.
JD558185 - Sequence 539209 from Patent EP1572962.
JD441031 - Sequence 422055 from Patent EP1572962.
JD412063 - Sequence 393087 from Patent EP1572962.
JD306073 - Sequence 287097 from Patent EP1572962.
JD276595 - Sequence 257619 from Patent EP1572962.
JD283083 - Sequence 264107 from Patent EP1572962.
JD096710 - Sequence 77734 from Patent EP1572962.
JD037844 - Sequence 18868 from Patent EP1572962.
JD042833 - Sequence 23857 from Patent EP1572962.
JD298872 - Sequence 279896 from Patent EP1572962.
JD563973 - Sequence 544997 from Patent EP1572962.
JD350398 - Sequence 331422 from Patent EP1572962.
JD324726 - Sequence 305750 from Patent EP1572962.
JD359328 - Sequence 340352 from Patent EP1572962.
JD146148 - Sequence 127172 from Patent EP1572962.
JD381372 - Sequence 362396 from Patent EP1572962.
JD063611 - Sequence 44635 from Patent EP1572962.
JD275450 - Sequence 256474 from Patent EP1572962.
JD352637 - Sequence 333661 from Patent EP1572962.
JD090759 - Sequence 71783 from Patent EP1572962.
JD159925 - Sequence 140949 from Patent EP1572962.
JD543872 - Sequence 524896 from Patent EP1572962.
JD430047 - Sequence 411071 from Patent EP1572962.
JD140416 - Sequence 121440 from Patent EP1572962.
JD138062 - Sequence 119086 from Patent EP1572962.
JD441879 - Sequence 422903 from Patent EP1572962.
JD147101 - Sequence 128125 from Patent EP1572962.
JD223627 - Sequence 204651 from Patent EP1572962.
JD427620 - Sequence 408644 from Patent EP1572962.
JD071040 - Sequence 52064 from Patent EP1572962.
JD159086 - Sequence 140110 from Patent EP1572962.
JD208997 - Sequence 190021 from Patent EP1572962.
JD279410 - Sequence 260434 from Patent EP1572962.
JD491326 - Sequence 472350 from Patent EP1572962.
JD244573 - Sequence 225597 from Patent EP1572962.
JD513049 - Sequence 494073 from Patent EP1572962.
JD424514 - Sequence 405538 from Patent EP1572962.
JD351839 - Sequence 332863 from Patent EP1572962.
JD201318 - Sequence 182342 from Patent EP1572962.
JD430234 - Sequence 411258 from Patent EP1572962.
JD557416 - Sequence 538440 from Patent EP1572962.
JD529274 - Sequence 510298 from Patent EP1572962.
JD169423 - Sequence 150447 from Patent EP1572962.
JD169424 - Sequence 150448 from Patent EP1572962.
JD041444 - Sequence 22468 from Patent EP1572962.
JD321182 - Sequence 302206 from Patent EP1572962.
JD388435 - Sequence 369459 from Patent EP1572962.
JD118497 - Sequence 99521 from Patent EP1572962.
JD366610 - Sequence 347634 from Patent EP1572962.
JD517797 - Sequence 498821 from Patent EP1572962.
JD517798 - Sequence 498822 from Patent EP1572962.
JD119896 - Sequence 100920 from Patent EP1572962.
JD562643 - Sequence 543667 from Patent EP1572962.
JD050021 - Sequence 31045 from Patent EP1572962.
MA619971 - JP 2018138019-A/191897: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q08477 (Reactome details) participates in the following event(s):

R-HSA-2161792 20cho-LTB4 is oxidised to 20cooh-LTB4 by CYP4F2/4F3
R-HSA-2161745 20oh-LTB4 is oxidised to 20cho-LTB4 by CYP4F2/4F3
R-HSA-211873 CYP4F2, 4F3 20-hydroxylate LTB4
R-HSA-215526 CYP4F3 20-hydroxylates LTB4
R-HSA-211924 CYP4B1 12-hydroxylates ARA
R-HSA-76466 CYP4A11 12-hydroxylates DDCX
R-HSA-211962 CYP4F11 16-hydroxylates 3OH-PALM
R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-HSA-211979 Eicosanoids
R-HSA-211935 Fatty acids
R-HSA-211958 Miscellaneous substrates
R-HSA-2142753 Arachidonic acid metabolism
R-HSA-211897 Cytochrome P450 - arranged by substrate type
R-HSA-8978868 Fatty acid metabolism
R-HSA-211945 Phase I - Functionalization of compounds
R-HSA-556833 Metabolism of lipids
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: B7Z8Z3, CP4F3_HUMAN, CYP4F3 , ENST00000221307.1, ENST00000221307.10, ENST00000221307.11, ENST00000221307.12, ENST00000221307.2, ENST00000221307.3, ENST00000221307.4, ENST00000221307.5, ENST00000221307.6, ENST00000221307.7, ENST00000221307.8, ENST00000221307.9, LTB4H, NM_000896, O60634, Q08477, Q5U740, uc317dar.1, uc317dar.2
UCSC ID: ENST00000221307.13_10
RefSeq Accession: NM_000896.3
Protein: Q08477 (aka CP4F3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.