Human Gene DAD1 (ENST00000250498.9_7) from GENCODE V47lift37
  Description: defender against cell death 1 (from RefSeq NM_001344.4)
Gencode Transcript: ENST00000250498.9_7
Gencode Gene: ENSG00000129562.11_9
Transcript (Including UTRs)
   Position: hg19 chr14:23,033,807-23,058,130 Size: 24,324 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg19 chr14:23,044,003-23,058,063 Size: 14,061 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:23,033,807-23,058,130)mRNA (may differ from genome)Protein (113 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DAD1_HUMAN
DESCRIPTION: RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; Short=Oligosaccharyl transferase subunit DAD1; EC=2.4.1.119; AltName: Full=Defender against cell death 1; Short=DAD-1;
FUNCTION: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis (By similarity).
CATALYTIC ACTIVITY: Dolichyl diphosphooligosaccharide + protein L- asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L- asparagine.
SUBUNIT: Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the DAD/OST2 family.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/dad1/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 162.21 RPKM in Cells - Cultured fibroblasts
Total median expression: 4736.42 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -18.0067-0.269 Picture PostScript Text
3' UTR -60.70275-0.221 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003038 - DAD

Pfam Domains:
PF02109 - DAD family

ModBase Predicted Comparative 3D Structure on P61803
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity
GO:0004576 oligosaccharyl transferase activity

Biological Process:
GO:0001824 blastocyst development
GO:0006486 protein glycosylation
GO:0006487 protein N-linked glycosylation
GO:0006915 apoptotic process
GO:0007584 response to nutrient
GO:0018279 protein N-linked glycosylation via asparagine
GO:0042493 response to drug
GO:0043066 negative regulation of apoptotic process

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0008250 oligosaccharyltransferase complex
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  BC038575 - Homo sapiens defender against cell death 1, mRNA (cDNA clone IMAGE:4538971), with apparent retained intron.
LF210064 - JP 2014500723-A/17567: Polycomb-Associated Non-Coding RNAs.
LF334398 - JP 2014500723-A/141901: Polycomb-Associated Non-Coding RNAs.
BC013646 - Homo sapiens cDNA clone IMAGE:3454611, **** WARNING: chimeric clone ****.
CQ903803 - Sequence 5 from Patent WO2004094661.
BC007403 - Homo sapiens defender against cell death 1, mRNA (cDNA clone MGC:2076 IMAGE:2988797), complete cds.
D15057 - Homo sapiens mRNA for DAD-1, complete cds.
AK223129 - Homo sapiens mRNA for defender against cell death 1 variant, clone: KAT11524.
BC009798 - Homo sapiens defender against cell death 1, mRNA (cDNA clone MGC:13634 IMAGE:4284779), complete cds.
JD503298 - Sequence 484322 from Patent EP1572962.
LF334399 - JP 2014500723-A/141902: Polycomb-Associated Non-Coding RNAs.
KJ896686 - Synthetic construct Homo sapiens clone ccsbBroadEn_06080 DAD1 gene, encodes complete protein.
CR542204 - Homo sapiens full open reading frame cDNA clone RZPDo834F1224D for gene DAD1, defender against cell death 1; complete cds, incl. stopcodon.
DQ892868 - Synthetic construct clone IMAGE:100005498; FLH190448.01X; RZPDo839A0676D defender against cell death 1 (DAD1) gene, encodes complete protein.
DQ896117 - Synthetic construct Homo sapiens clone IMAGE:100010577; FLH190444.01L; RZPDo839A0666D defender against cell death 1 (DAD1) gene, encodes complete protein.
CR407682 - Homo sapiens full open reading frame cDNA clone RZPDo834D023D for gene DAD1, defender against cell death 1 complete cds, without stopcodon.
LF334402 - JP 2014500723-A/141905: Polycomb-Associated Non-Coding RNAs.
LF334403 - JP 2014500723-A/141906: Polycomb-Associated Non-Coding RNAs.
LF334409 - JP 2014500723-A/141912: Polycomb-Associated Non-Coding RNAs.
LF210066 - JP 2014500723-A/17569: Polycomb-Associated Non-Coding RNAs.
MA445641 - JP 2018138019-A/17567: Polycomb-Associated Non-Coding RNAs.
MA569975 - JP 2018138019-A/141901: Polycomb-Associated Non-Coding RNAs.
MA569976 - JP 2018138019-A/141902: Polycomb-Associated Non-Coding RNAs.
MA569979 - JP 2018138019-A/141905: Polycomb-Associated Non-Coding RNAs.
MA569980 - JP 2018138019-A/141906: Polycomb-Associated Non-Coding RNAs.
MA569986 - JP 2018138019-A/141912: Polycomb-Associated Non-Coding RNAs.
MA445643 - JP 2018138019-A/17569: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
MANNOSYL-CHITO-DOLICHOL-BIOSYNTHESIS - protein N-glycosylation initial phase (eukaryotic)

Reactome (by CSHL, EBI, and GO)

Protein P61803 (Reactome details) participates in the following event(s):

R-HSA-446209 Transfer of N-glycan to the protein
R-HSA-446203 Asparagine N-linked glycosylation
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: D3DS25, DAD1 , DAD1_HUMAN, ENST00000250498.1, ENST00000250498.2, ENST00000250498.3, ENST00000250498.4, ENST00000250498.5, ENST00000250498.6, ENST00000250498.7, ENST00000250498.8, NM_001344, O08552, O70364, P46966, P46968, P61803, Q6FGA3, Q96GB7, uc317fbw.1, uc317fbw.2
UCSC ID: ENST00000250498.9_7
RefSeq Accession: NM_001344.4
Protein: P61803 (aka DAD1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.