Human Gene DDX27 (ENST00000618172.5_9) from GENCODE V47lift37
  Description: DEAD-box helicase 27, transcript variant 2 (from RefSeq NM_017895.8)
Gencode Transcript: ENST00000618172.5_9
Gencode Gene: ENSG00000124228.15_14
Transcript (Including UTRs)
   Position: hg19 chr20:47,835,953-47,860,610 Size: 24,658 Total Exon Count: 21 Strand: +
Coding Region
   Position: hg19 chr20:47,835,986-47,860,371 Size: 24,386 Coding Exon Count: 21 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr20:47,835,953-47,860,610)mRNA (may differ from genome)Protein (765 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: B7Z6D5_HUMAN
DESCRIPTION: SubName: Full=cDNA FLJ61659, highly similar to Probable ATP-dependent RNA helicase DDX27 (EC 3.6.1.-);
SIMILARITY: Belongs to the DEAD box helicase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -7.0033-0.212 Picture PostScript Text
3' UTR -55.30239-0.231 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011545 - DNA/RNA_helicase_DEAD/DEAH_N
IPR014001 - Helicase_ATP-bd
IPR001650 - Helicase_C
IPR000629 - RNA-helicase_DEAD-box_CS
IPR014014 - RNA_helicase_DEAD_Q_motif

Pfam Domains:
PF00270 - DEAD/DEAH box helicase
PF00271 - Helicase conserved C-terminal domain
PF04851 - Type III restriction enzyme, res subunit

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases

ModBase Predicted Comparative 3D Structure on B7Z6D5
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0016787 hydrolase activity


-  Descriptions from all associated GenBank mRNAs
  BC126287 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone MGC:161565 IMAGE:8992003), complete cds.
BC130275 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone MGC:163147 IMAGE:40146306), complete cds.
BC144125 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone MGC:177662 IMAGE:9052645), complete cds.
HQ258508 - Synthetic construct Homo sapiens clone IMAGE:100072937 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27) gene, encodes complete protein.
KJ899168 - Synthetic construct Homo sapiens clone ccsbBroadEn_08562 DDX27 gene, encodes complete protein.
AY044431 - Homo sapiens RNA helicase-like protein mRNA, complete cds.
AL832131 - Homo sapiens mRNA; cDNA DKFZp686H1510 (from clone DKFZp686H1510).
EU718675 - Homo sapiens DEAD box polypeptide 27 (DDX27) mRNA, complete cds.
AK300135 - Homo sapiens cDNA FLJ61659 complete cds, highly similar to Probable ATP-dependent RNA helicase DDX27 (EC 3.6.1.-).
AK022979 - Homo sapiens cDNA FLJ12917 fis, clone NT2RP2004568, weakly similar to PUTATIVE ATP-DEPENDENT RNA HELICASE C30D11.03.
BC009304 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone IMAGE:4128730), partial cds.
BC016060 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone IMAGE:4903119), partial cds.
BC011927 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone IMAGE:4544412), partial cds.
HQ258546 - Synthetic construct Homo sapiens clone IMAGE:100072975 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27) gene, encodes complete protein.
KJ906237 - Synthetic construct Homo sapiens clone ccsbBroadEn_15907 DDX27 gene, encodes complete protein.
AF193054 - Homo sapiens PP3241 mRNA, complete cds.
BC104480 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27, mRNA (cDNA clone IMAGE:6387875), partial cds.
AK025891 - Homo sapiens cDNA: FLJ22238 fis, clone HRC02173, highly similar to AF161377 Homo sapiens HSPC259 mRNA.
AF336851 - Homo sapiens RNA helicase-like protein (RHLP) mRNA, complete cds.
AK000603 - Homo sapiens cDNA FLJ20596 fis, clone KAT08049.
AK223116 - Homo sapiens mRNA for DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 variant, clone: KAT08049.
AK127128 - Homo sapiens cDNA FLJ45185 fis, clone BRAWH3048317, highly similar to ATP dependent RNA helicase.
AL512707 - Homo sapiens mRNA; cDNA DKFZp667N057 (from clone DKFZp667N057).
AF161377 - Homo sapiens HSPC259 mRNA, partial cds.
JD544308 - Sequence 525332 from Patent EP1572962.
JD104795 - Sequence 85819 from Patent EP1572962.
JD401366 - Sequence 382390 from Patent EP1572962.
JD548437 - Sequence 529461 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B7Z6D5, B7Z6D5_HUMAN, DDX27 , ENST00000618172.1, ENST00000618172.2, ENST00000618172.3, ENST00000618172.4, NM_017895, uc327rol.1, uc327rol.2
UCSC ID: ENST00000618172.5_9
RefSeq Accession: NM_017895.8
Protein: B7Z6D5

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.