Human Gene DNAJA1 (ENST00000330899.5_4) from GENCODE V47lift37
  Description: DnaJ heat shock protein family (Hsp40) member A1, transcript variant 1 (from RefSeq NM_001539.4)
Gencode Transcript: ENST00000330899.5_4
Gencode Gene: ENSG00000086061.16_7
Transcript (Including UTRs)
   Position: hg19 chr9:33,025,271-33,039,905 Size: 14,635 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg19 chr9:33,026,483-33,038,901 Size: 12,419 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:33,025,271-33,039,905)mRNA (may differ from genome)Protein (397 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DNJA1_HUMAN
DESCRIPTION: RecName: Full=DnaJ homolog subfamily A member 1; AltName: Full=DnaJ protein homolog 2; AltName: Full=HSDJ; AltName: Full=Heat shock 40 kDa protein 4; AltName: Full=Heat shock protein J2; Short=HSJ-2; AltName: Full=Human DnaJ protein 2; Short=hDj-2; Flags: Precursor;
FUNCTION: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
SUBCELLULAR LOCATION: Membrane; Lipid-anchor (Potential).
SIMILARITY: Contains 1 CR-type zinc finger.
SIMILARITY: Contains 1 J domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 105.02 RPKM in Testis
Total median expression: 2364.23 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -44.00121-0.364 Picture PostScript Text
3' UTR -251.601004-0.251 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012724 - DnaJ
IPR002939 - DnaJ_C
IPR001623 - DnaJ_N
IPR018253 - Heat_shock_DnaJ_CS
IPR008971 - HSP40/DnaJ_pept-bd
IPR003095 - Hsp_DnaJ
IPR001305 - HSP_DnaJ_Cys-rich_dom

Pfam Domains:
PF00226 - DnaJ domain
PF00684 - DnaJ central domain
PF01556 - DnaJ C terminal domain

SCOP Domains:
46565 - Chaperone J-domain
49493 - HSP40/DnaJ peptide-binding domain
57938 - DnaJ/Hsp40 cysteine-rich domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2LO1 - NMR MuPIT


ModBase Predicted Comparative 3D Structure on P31689
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001664 G-protein coupled receptor binding
GO:0001671 ATPase activator activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0030544 Hsp70 protein binding
GO:0030957 Tat protein binding
GO:0031072 heat shock protein binding
GO:0031625 ubiquitin protein ligase binding
GO:0046872 metal ion binding
GO:0050750 low-density lipoprotein particle receptor binding
GO:0051082 unfolded protein binding
GO:0051087 chaperone binding
GO:0055131 C3HC4-type RING finger domain binding

Biological Process:
GO:0006457 protein folding
GO:0006986 response to unfolded protein
GO:0007283 spermatogenesis
GO:0009408 response to heat
GO:0030317 flagellated sperm motility
GO:0030521 androgen receptor signaling pathway
GO:0031397 negative regulation of protein ubiquitination
GO:0032781 positive regulation of ATPase activity
GO:0042769 DNA damage response, detection of DNA damage
GO:0043065 positive regulation of apoptotic process
GO:0043066 negative regulation of apoptotic process
GO:0043508 negative regulation of JUN kinase activity
GO:0051223 regulation of protein transport
GO:0070585 protein localization to mitochondrion
GO:1901998 toxin transport
GO:1903748 negative regulation of establishment of protein localization to mitochondrion
GO:1905259 negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005783 endoplasmic reticulum
GO:0005829 cytosol
GO:0015630 microtubule cytoskeleton
GO:0016020 membrane
GO:0043231 intracellular membrane-bounded organelle
GO:0048471 perinuclear region of cytoplasm
GO:0070062 extracellular exosome
GO:0098554 cytoplasmic side of endoplasmic reticulum membrane
GO:0000151 ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  AK298725 - Homo sapiens cDNA FLJ52352 complete cds, highly similar to DnaJ homolog subfamily A member 1.
AK289623 - Homo sapiens cDNA FLJ76588 complete cds, highly similar to Homo sapiens DnaJ (Hsp40) homolog, subfamily A, member 1 (DNAJA1), mRNA.
AY186741 - Homo sapiens DnaJA2 mRNA, complete cds; alternatively spliced.
D13388 - Homo sapiens mRNA for DnaJ protein homolog, complete cds.
BC008182 - Homo sapiens DnaJ (Hsp40) homolog, subfamily A, member 1, mRNA (cDNA clone MGC:5217 IMAGE:2900892), complete cds.
L08069 - Human heat shock protein, E. coli DnaJ homologue mRNA, complete cds.
EU176556 - Synthetic construct Homo sapiens clone IMAGE:100011373; FLH169265.01L; RZPDo839E10253D DnaJ (Hsp40) homolog, subfamily A, member 1 (DNAJA1) gene, encodes complete protein.
BT007292 - Homo sapiens DnaJ (Hsp40) homolog, subfamily A, member 1 mRNA, complete cds.
DQ891091 - Synthetic construct clone IMAGE:100003721; FLH169266.01X; RZPDo839A0596D DnaJ (Hsp40) homolog, subfamily A, member 1 (DNAJA1) gene, encodes complete protein.
KJ897011 - Synthetic construct Homo sapiens clone ccsbBroadEn_06405 DNAJA1 gene, encodes complete protein.
CU678881 - Synthetic construct Homo sapiens gateway clone IMAGE:100017179 5' read DNAJA1 mRNA.
AJ277242 - Homo sapiens partial mRNA for HDJ2 protein.
JD126669 - Sequence 107693 from Patent EP1572962.
JD453764 - Sequence 434788 from Patent EP1572962.
DQ581549 - Homo sapiens piRNA piR-49661, complete sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P31689 (Reactome details) participates in the following event(s):

R-HSA-3371503 STIP1(HOP) binds HSP90 and HSP70:HSP40:nascent protein
R-HSA-3371422 ATP hydrolysis by HSP70
R-HSA-3371590 HSP70 binds to HSP40:nascent protein
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-2262752 Cellular responses to stress
R-HSA-8953897 Cellular responses to external stimuli

-  Other Names for This Gene
  Alternate Gene Symbols: DNAJ2, DNJA1_HUMAN, ENST00000330899.1, ENST00000330899.2, ENST00000330899.3, ENST00000330899.4, HDJ2, HSJ2, HSPF4, NM_001539, P31689, Q5T7Q0, Q86TL9, uc317tbv.1, uc317tbv.2
UCSC ID: ENST00000330899.5_4
RefSeq Accession: NM_001539.4
Protein: P31689 (aka DNJA1_HUMAN or DJA1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.