Human Gene DNAJC8 (ENST00000263697.6_8) from GENCODE V47lift37
  Description: DnaJ heat shock protein family (Hsp40) member C8, transcript variant 1 (from RefSeq NM_014280.3)
Gencode Transcript: ENST00000263697.6_8
Gencode Gene: ENSG00000126698.11_12
Transcript (Including UTRs)
   Position: hg19 chr1:28,526,789-28,559,540 Size: 32,752 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg19 chr1:28,527,759-28,559,509 Size: 31,751 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:28,526,789-28,559,540)mRNA (may differ from genome)Protein (253 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DNJC8_HUMAN
DESCRIPTION: RecName: Full=DnaJ homolog subfamily C member 8; AltName: Full=Splicing protein spf31;
SIMILARITY: Contains 1 J domain.
SEQUENCE CAUTION: Sequence=AAC35352.1; Type=Erroneous initiation; Sequence=CAG33181.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 38.44 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 1016.55 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -6.5031-0.210 Picture PostScript Text
3' UTR -277.30970-0.286 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001623 - DnaJ_N
IPR003095 - Hsp_DnaJ

Pfam Domains:
PF00226 - DnaJ domain

SCOP Domains:
46565 - Chaperone J-domain

ModBase Predicted Comparative 3D Structure on O75937
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsembl WormBase 
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0030544 Hsp70 protein binding

Biological Process:
GO:0000398 mRNA splicing, via spliceosome

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005829 cytosol
GO:0045171 intercellular bridge


-  Descriptions from all associated GenBank mRNAs
  AF161449 - Homo sapiens HSPC331 mRNA, partial cds.
AF161433 - Homo sapiens HSPC315 mRNA, partial cds.
BC033159 - Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 8, mRNA (cDNA clone MGC:45787 IMAGE:4330676), complete cds.
AF083190 - Homo sapiens SPF31 (SPF31) mRNA, complete cds.
BX538122 - Homo sapiens mRNA; cDNA DKFZp686P08160 (from clone DKFZp686P08160).
JD377546 - Sequence 358570 from Patent EP1572962.
JD454939 - Sequence 435963 from Patent EP1572962.
JD271151 - Sequence 252175 from Patent EP1572962.
JD203190 - Sequence 184214 from Patent EP1572962.
JD043041 - Sequence 24065 from Patent EP1572962.
JD289226 - Sequence 270250 from Patent EP1572962.
AK300797 - Homo sapiens cDNA FLJ61418 complete cds, highly similar to DnaJ homolog subfamily C member 8.
JD121389 - Sequence 102413 from Patent EP1572962.
JD252010 - Sequence 233034 from Patent EP1572962.
JD259825 - Sequence 240849 from Patent EP1572962.
JD189370 - Sequence 170394 from Patent EP1572962.
JD288879 - Sequence 269903 from Patent EP1572962.
JD390949 - Sequence 371973 from Patent EP1572962.
JD392905 - Sequence 373929 from Patent EP1572962.
JD554856 - Sequence 535880 from Patent EP1572962.
JD102498 - Sequence 83522 from Patent EP1572962.
JD381133 - Sequence 362157 from Patent EP1572962.
JD230510 - Sequence 211534 from Patent EP1572962.
JD180990 - Sequence 162014 from Patent EP1572962.
KJ898410 - Synthetic construct Homo sapiens clone ccsbBroadEn_07804 DNAJC8 gene, encodes complete protein.
CR456900 - Homo sapiens full open reading frame cDNA clone RZPDo834B047D for gene DNAJC8, DnaJ (Hsp40) homolog, subfamily C, member 8; complete cds, incl. stopcodon.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O75937 (Reactome details) participates in the following event(s):

R-HSA-72124 Formation of the Spliceosomal A Complex
R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage
R-HSA-72139 Formation of the active Spliceosomal C (B*) complex
R-HSA-72127 Formation of the Spliceosomal B Complex
R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex
R-HSA-156661 Formation of Exon Junction Complex
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72172 mRNA Splicing
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-8953854 Metabolism of RNA

-  Other Names for This Gene
  Alternate Gene Symbols: B4DUU4, D3DPM0, DNJC8_HUMAN, ENST00000263697.1, ENST00000263697.2, ENST00000263697.3, ENST00000263697.4, ENST00000263697.5, HSPC315, HSPC331, NM_014280, O75937, Q6IBA4, Q8N4Z5, Q9P051, Q9P067, SPF31, uc317hls.1, uc317hls.2
UCSC ID: ENST00000263697.6_8
RefSeq Accession: NM_014280.3
Protein: O75937 (aka DNJC8_HUMAN or DJC8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.