Human Gene DROSHA (ENST00000344624.8_11) from GENCODE V47lift37
  Description: drosha ribonuclease III, transcript variant 3 (from RefSeq NM_001382508.1)
Gencode Transcript: ENST00000344624.8_11
Gencode Gene: ENSG00000113360.17_16
Transcript (Including UTRs)
   Position: hg19 chr5:31,400,601-31,532,200 Size: 131,600 Total Exon Count: 36 Strand: -
Coding Region
   Position: hg19 chr5:31,401,539-31,529,166 Size: 127,628 Coding Exon Count: 33 

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Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:31,400,601-31,532,200)mRNA (may differ from genome)Protein (1374 aa)
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BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
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WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RNC_HUMAN
DESCRIPTION: RecName: Full=Ribonuclease 3; EC=3.1.26.3; AltName: Full=Protein Drosha; AltName: Full=Ribonuclease III; Short=RNase III; AltName: Full=p241;
FUNCTION: Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA- ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double- strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'- phosphomonoester.
COFACTOR: Magnesium or manganese (By similarity).
SUBUNIT: Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex may contain multiple subunit of DGCR8 and DROSHA. Interacts with DGCR8, SP1 and SNIP1. Interacts with SRRT/ARS2.
INTERACTION: Q8WYQ5:DGCR8; NbExp=4; IntAct=EBI-528367, EBI-528411;
SUBCELLULAR LOCATION: Nucleus. Nucleus, nucleolus. Note=A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN: The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.
SIMILARITY: Contains 1 DRBM (double-stranded RNA-binding) domain.
SIMILARITY: Contains 2 RNase III domains.
SEQUENCE CAUTION: Sequence=AAD29637.1; Type=Frameshift; Positions=775; Sequence=BAA91511.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
WEB RESOURCE: Name=Protein Spotlight; Note=The dark side of RNA - Issue 87 of October 2007; URL="http://web.expasy.org/spotlight/back_issues/sptlt087.shtml";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: DROSHA
Diseases sorted by gene-association score: combat disorder (7), murray valley encephalitis (7), ovarian serous carcinoma (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 15.40 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 421.10 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -115.10353-0.326 Picture PostScript Text
3' UTR -220.60938-0.235 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001159 - Ds-RNA-bd
IPR014720 - dsRNA-bd-like
IPR011907 - RNase_III
IPR000999 - RNase_III_dom

Pfam Domains:
PF00035 - Double-stranded RNA binding motif
PF00636 - Ribonuclease III domain
PF14622 - Ribonuclease-III-like

SCOP Domains:
48452 - TPR-like
69065 - RNase III domain-like
54768 - dsRNA-binding domain-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2KHX - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q9NRR4
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001530 lipopolysaccharide binding
GO:0003723 RNA binding
GO:0003725 double-stranded RNA binding
GO:0004518 nuclease activity
GO:0004519 endonuclease activity
GO:0004521 endoribonuclease activity
GO:0004525 ribonuclease III activity
GO:0005515 protein binding
GO:0016787 hydrolase activity
GO:0017151 DEAD/H-box RNA helicase binding
GO:0042803 protein homodimerization activity
GO:0046332 SMAD binding
GO:0046872 metal ion binding
GO:0070412 R-SMAD binding
GO:0070878 primary miRNA binding

Biological Process:
GO:0006396 RNA processing
GO:0010468 regulation of gene expression
GO:0010586 miRNA metabolic process
GO:0010628 positive regulation of gene expression
GO:0016075 rRNA catabolic process
GO:0030422 production of siRNA involved in RNA interference
GO:0031047 gene silencing by RNA
GO:0031053 primary miRNA processing
GO:0031054 pre-miRNA processing
GO:0042254 ribosome biogenesis
GO:0045589 regulation of regulatory T cell differentiation
GO:0050727 regulation of inflammatory response
GO:0050829 defense response to Gram-negative bacterium
GO:0050830 defense response to Gram-positive bacterium
GO:0090305 nucleic acid phosphodiester bond hydrolysis
GO:0090501 RNA phosphodiester bond hydrolysis
GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
GO:2000628 regulation of miRNA metabolic process

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0014069 postsynaptic density
GO:0070877 microprocessor complex


-  Descriptions from all associated GenBank mRNAs
  AF189011 - Homo sapiens ribonuclease III (RN3) mRNA, complete cds.
BC024261 - Homo sapiens ribonuclease III, nuclear, mRNA (cDNA clone IMAGE:3631271).
BC041162 - Homo sapiens ribonuclease type III, nuclear, mRNA (cDNA clone IMAGE:5114643), partial cds.
BC054003 - Homo sapiens ribonuclease type III, nuclear, mRNA (cDNA clone MGC:61568 IMAGE:6148714), complete cds.
BX647724 - Homo sapiens mRNA; cDNA DKFZp686F16262 (from clone DKFZp686F16262).
AF116910 - Homo sapiens clone HAW100 putative ribonuclease III mRNA, complete cds.
AK001121 - Homo sapiens cDNA FLJ10259 fis, clone HEMBB1000947, highly similar to Homo sapiens clone HAW100 putative ribonuclease III mRNA.
AK308093 - Homo sapiens cDNA, FLJ98041.
AB209501 - Homo sapiens mRNA for nuclear RNase III Drosha variant protein.
JD326879 - Sequence 307903 from Patent EP1572962.
AK026563 - Homo sapiens cDNA: FLJ22910 fis, clone KAT05748, highly similar to AF116910 Homo sapiens clone HAW100 putative ribonuclease III mRNA.
JD088094 - Sequence 69118 from Patent EP1572962.
AJ242976 - Homo sapiens partial mRNA for putative protein p241 which interacts with transcription factor Sp1.
JD326376 - Sequence 307400 from Patent EP1572962.
JD385685 - Sequence 366709 from Patent EP1572962.
JD538283 - Sequence 519307 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9NRR4 (Reactome details) participates in the following event(s):

R-HSA-203893 Microprocessor complex cleaves pri-miRNA to pre-miRNA
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-211000 Gene Silencing by RNA
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: E7EMP9, ENST00000344624.1, ENST00000344624.2, ENST00000344624.3, ENST00000344624.4, ENST00000344624.5, ENST00000344624.6, ENST00000344624.7, NM_001382508, Q7Z5V2, Q86YH0, Q9NRR4, Q9NW73, Q9Y2V9, Q9Y4Y0, RN3, RNASE3L, RNASEN, RNC_HUMAN, uc317wzv.1, uc317wzv.2
UCSC ID: ENST00000344624.8_11
RefSeq Accession: NM_001382508.1
Protein: Q9NRR4 (aka RNC_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.