Human Gene DUSP18 (ENST00000334679.4_7) from GENCODE V47lift37
  Description: dual specificity phosphatase 18, transcript variant 2 (from RefSeq NM_152511.5)
Gencode Transcript: ENST00000334679.4_7
Gencode Gene: ENSG00000167065.14_17
Transcript (Including UTRs)
   Position: hg19 chr22:31,057,432-31,063,862 Size: 6,431 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr22:31,059,424-31,059,990 Size: 567 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:31,057,432-31,063,862)mRNA (may differ from genome)Protein (188 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DUS18_HUMAN
DESCRIPTION: RecName: Full=Dual specificity protein phosphatase 18; EC=3.1.3.16; EC=3.1.3.48; AltName: Full=Low molecular weight dual specificity phosphatase 20; Short=LMW-DSP20;
FUNCTION: Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP).
CATALYTIC ACTIVITY: Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.
CATALYTIC ACTIVITY: A phosphoprotein + H(2)O = a protein + phosphate.
ENZYME REGULATION: Activated by manganese ions, inhibited by iodoaretic acid.
BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0; Temperature dependence: Optimum temperature is 55 degrees Celsius;
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Widely expressed with highest levels in liver, brain, ovary and testis.
SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
SIMILARITY: Contains 1 tyrosine-protein phosphatase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.23 RPKM in Testis
Total median expression: 62.26 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -212.40491-0.433 Picture PostScript Text
3' UTR -629.101992-0.316 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020417 - Atypical_DUSP
IPR020420 - Atypical_DUSP_famB
IPR000340 - Dual-sp_phosphatase_cat-dom
IPR020422 - Dual-sp_phosphatase_subgr_cat
IPR024950 - DUSP
IPR000387 - Tyr/Dual-specificity_Pase
IPR016130 - Tyr_Pase_AS

Pfam Domains:
PF00782 - Dual specificity phosphatase, catalytic domain

SCOP Domains:
52799 - (Phosphotyrosine protein) phosphatases II
53697 - SIS domain
160930 - FlhC-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2ESB - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q8NEJ0
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004721 phosphoprotein phosphatase activity
GO:0004725 protein tyrosine phosphatase activity
GO:0008138 protein tyrosine/serine/threonine phosphatase activity
GO:0016787 hydrolase activity
GO:0016791 phosphatase activity
GO:0017017 MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process:
GO:0000188 inactivation of MAPK activity
GO:0006470 protein dephosphorylation
GO:0016311 dephosphorylation
GO:0035335 peptidyl-tyrosine dephosphorylation
GO:0035970 peptidyl-threonine dephosphorylation

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  BC030987 - Homo sapiens dual specificity phosphatase 18, mRNA (cDNA clone MGC:32658 IMAGE:4730282), complete cds.
BC028724 - Homo sapiens dual specificity phosphatase 18, mRNA (cDNA clone IMAGE:5269586).
JD243317 - Sequence 224341 from Patent EP1572962.
JD375202 - Sequence 356226 from Patent EP1572962.
JD238503 - Sequence 219527 from Patent EP1572962.
JD238501 - Sequence 219525 from Patent EP1572962.
JD238502 - Sequence 219526 from Patent EP1572962.
JD058299 - Sequence 39323 from Patent EP1572962.
JD220281 - Sequence 201305 from Patent EP1572962.
JD101054 - Sequence 82078 from Patent EP1572962.
JD544979 - Sequence 526003 from Patent EP1572962.
JD223277 - Sequence 204301 from Patent EP1572962.
AK025036 - Homo sapiens cDNA: FLJ21383 fis, clone COL03345.
BC004110 - Homo sapiens dual specificity phosphatase 18, mRNA (cDNA clone IMAGE:3689593), partial cds.
AF461689 - Homo sapiens dual-specificity phosphatase-like protein mRNA, complete cds.
AK056074 - Homo sapiens cDNA FLJ31512 fis, clone NT2RI1000048, highly similar to Dual specificity protein phosphatase 18 (EC 3.1.3.48).
LF384703 - JP 2014500723-A/192206: Polycomb-Associated Non-Coding RNAs.
JD275577 - Sequence 256601 from Patent EP1572962.
JD083015 - Sequence 64039 from Patent EP1572962.
JD078734 - Sequence 59758 from Patent EP1572962.
JD424235 - Sequence 405259 from Patent EP1572962.
DQ579485 - Homo sapiens piRNA piR-47597, complete sequence.
JD246470 - Sequence 227494 from Patent EP1572962.
JD336316 - Sequence 317340 from Patent EP1572962.
JD403342 - Sequence 384366 from Patent EP1572962.
JD280283 - Sequence 261307 from Patent EP1572962.
JD550165 - Sequence 531189 from Patent EP1572962.
JD159413 - Sequence 140437 from Patent EP1572962.
JD184523 - Sequence 165547 from Patent EP1572962.
JD147121 - Sequence 128145 from Patent EP1572962.
JD504936 - Sequence 485960 from Patent EP1572962.
JD203732 - Sequence 184756 from Patent EP1572962.
JD480197 - Sequence 461221 from Patent EP1572962.
JD524851 - Sequence 505875 from Patent EP1572962.
JD368139 - Sequence 349163 from Patent EP1572962.
JD463338 - Sequence 444362 from Patent EP1572962.
JD213442 - Sequence 194466 from Patent EP1572962.
JD543014 - Sequence 524038 from Patent EP1572962.
JD355689 - Sequence 336713 from Patent EP1572962.
JD250134 - Sequence 231158 from Patent EP1572962.
JD085210 - Sequence 66234 from Patent EP1572962.
JD158263 - Sequence 139287 from Patent EP1572962.
JD160952 - Sequence 141976 from Patent EP1572962.
JD122564 - Sequence 103588 from Patent EP1572962.
JD492108 - Sequence 473132 from Patent EP1572962.
JD546531 - Sequence 527555 from Patent EP1572962.
JD160951 - Sequence 141975 from Patent EP1572962.
JD346999 - Sequence 328023 from Patent EP1572962.
JD412077 - Sequence 393101 from Patent EP1572962.
JD372612 - Sequence 353636 from Patent EP1572962.
JD076096 - Sequence 57120 from Patent EP1572962.
JD546379 - Sequence 527403 from Patent EP1572962.
JD059218 - Sequence 40242 from Patent EP1572962.
AF533017 - Homo sapiens low molecular weight dual specificity phosphatase 20 (LMWDSP20) mRNA, complete cds.
JD269170 - Sequence 250194 from Patent EP1572962.
CR456406 - Homo sapiens bK963H5.1 full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.bK963H5.1).
CU012964 - Homo sapiens DUSP18, mRNA (cDNA clone IMAGE:100000126), complete cds, with stop codon, in Gateway system.
AB463586 - Synthetic construct DNA, clone: pF1KB7095, Homo sapiens DUSP18 gene for dual specificity phosphatase 18, without stop codon, in Flexi system.
CU013252 - Homo sapiens DUSP18, mRNA (cDNA clone IMAGE:100000030), complete cds, without stop codon, in Gateway system.
KJ903868 - Synthetic construct Homo sapiens clone ccsbBroadEn_13262 DUSP18 gene, encodes complete protein.
DQ580875 - Homo sapiens piRNA piR-48987, complete sequence.
JD499317 - Sequence 480341 from Patent EP1572962.
DQ585636 - Homo sapiens piRNA piR-52748, complete sequence.
JD443120 - Sequence 424144 from Patent EP1572962.
JD259438 - Sequence 240462 from Patent EP1572962.
JD540590 - Sequence 521614 from Patent EP1572962.
JD405133 - Sequence 386157 from Patent EP1572962.
JD218400 - Sequence 199424 from Patent EP1572962.
JD158383 - Sequence 139407 from Patent EP1572962.
JD061105 - Sequence 42129 from Patent EP1572962.
JD441965 - Sequence 422989 from Patent EP1572962.
MA620280 - JP 2018138019-A/192206: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: B3KPA4, DUS18_HUMAN, ENST00000334679.1, ENST00000334679.2, ENST00000334679.3, LMWDSP20, NM_152511, Q8NEJ0, uc317ubb.1, uc317ubb.2
UCSC ID: ENST00000334679.4_7
RefSeq Accession: NM_152511.5
Protein: Q8NEJ0 (aka DUS18_HUMAN or DUSI_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.