Human Gene DUSP6 (ENST00000279488.8_7) from GENCODE V47lift37
  Description: dual specificity phosphatase 6, transcript variant 1 (from RefSeq NM_001946.4)
Gencode Transcript: ENST00000279488.8_7
Gencode Gene: ENSG00000139318.8_11
Transcript (Including UTRs)
   Position: hg19 chr12:89,741,012-89,746,278 Size: 5,267 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg19 chr12:89,743,031-89,745,816 Size: 2,786 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesGeneReviewsModel Information
Methods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:89,741,012-89,746,278)mRNA (may differ from genome)Protein (381 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DUS6_HUMAN
DESCRIPTION: RecName: Full=Dual specificity protein phosphatase 6; EC=3.1.3.16; EC=3.1.3.48; AltName: Full=Dual specificity protein phosphatase PYST1; AltName: Full=Mitogen-activated protein kinase phosphatase 3; Short=MAP kinase phosphatase 3; Short=MKP-3;
FUNCTION: Inactivates MAP kinases. Has a specificity for the ERK family.
CATALYTIC ACTIVITY: Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.
CATALYTIC ACTIVITY: A phosphoprotein + H(2)O = a protein + phosphate.
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
SIMILARITY: Contains 1 rhodanese domain.
SIMILARITY: Contains 1 tyrosine-protein phosphatase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: DUSP6
Diseases sorted by gene-association score: hypogonadotropic hypogonadism 19 with or without anosmia* (1255), normosmic congenital hypogonadotropic hypogonadism* (90), kallmann syndrome* (81), hypogonadotropic hypogonadism (16), hypogonadism (14)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 33.36 RPKM in Adipose - Visceral (Omentum)
Total median expression: 465.49 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -196.60462-0.426 Picture PostScript Text
3' UTR -516.592019-0.256 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000340 - Dual-sp_phosphatase_cat-dom
IPR020422 - Dual-sp_phosphatase_subgr_cat
IPR024950 - DUSP
IPR008343 - MKP
IPR001763 - Rhodanese-like_dom
IPR000387 - Tyr/Dual-specificity_Pase

Pfam Domains:
PF00581 - Rhodanese-like domain
PF00782 - Dual specificity phosphatase, catalytic domain

SCOP Domains:
52799 - (Phosphotyrosine protein) phosphatases II
52821 - Rhodanese/Cell cycle control phosphatase

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1HZM - NMR MuPIT 1MKP - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q16828
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004721 phosphoprotein phosphatase activity
GO:0004725 protein tyrosine phosphatase activity
GO:0008138 protein tyrosine/serine/threonine phosphatase activity
GO:0016787 hydrolase activity
GO:0016791 phosphatase activity
GO:0017017 MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process:
GO:0000165 MAPK cascade
GO:0000187 activation of MAPK activity
GO:0000188 inactivation of MAPK activity
GO:0001933 negative regulation of protein phosphorylation
GO:0006470 protein dephosphorylation
GO:0009953 dorsal/ventral pattern formation
GO:0010033 response to organic substance
GO:0010942 positive regulation of cell death
GO:0014070 response to organic cyclic compound
GO:0016311 dephosphorylation
GO:0030154 cell differentiation
GO:0035335 peptidyl-tyrosine dephosphorylation
GO:0040036 regulation of fibroblast growth factor receptor signaling pathway
GO:0042493 response to drug
GO:0042663 regulation of endodermal cell fate specification
GO:0043065 positive regulation of apoptotic process
GO:0051409 response to nitrosative stress
GO:0060420 regulation of heart growth
GO:0070373 negative regulation of ERK1 and ERK2 cascade
GO:0070848 response to growth factor

Cellular Component:
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  LP882414 - Sequence 9 from Patent WO2017180581.
BC003143 - Homo sapiens dual specificity phosphatase 6, mRNA (cDNA clone MGC:3789 IMAGE:2906126), complete cds.
BC003562 - Homo sapiens dual specificity phosphatase 6, mRNA (cDNA clone MGC:1954 IMAGE:3547162), complete cds.
BC037236 - Homo sapiens dual specificity phosphatase 6, mRNA (cDNA clone MGC:35368 IMAGE:5183133), complete cds.
AB013382 - Homo sapiens mRNA for DUSP6, complete cds.
AB013602 - Homo sapiens mRNA for DUSP6-ALT, complete cds.
AK091753 - Homo sapiens cDNA FLJ34434 fis, clone HLUNG2000950, highly similar to DUAL SPECIFICITY PROTEIN PHOSPHATASE 6 (EC 3.1.3.48).
JD225900 - Sequence 206924 from Patent EP1572962.
BC005047 - Homo sapiens dual specificity phosphatase 6, mRNA (cDNA clone MGC:12852 IMAGE:3954486), complete cds.
X93920 - H.sapiens mRNA for protein-tyrosine-phosphatase (tissue type: foreskin).
AK222882 - Homo sapiens mRNA for dual specificity phosphatase 6 isoform b variant, clone: HRC00358.
JD557770 - Sequence 538794 from Patent EP1572962.
JD177396 - Sequence 158420 from Patent EP1572962.
JD237346 - Sequence 218370 from Patent EP1572962.
JD459449 - Sequence 440473 from Patent EP1572962.
JD176591 - Sequence 157615 from Patent EP1572962.
JD097571 - Sequence 78595 from Patent EP1572962.
JD121294 - Sequence 102318 from Patent EP1572962.
JD123158 - Sequence 104182 from Patent EP1572962.
JD260612 - Sequence 241636 from Patent EP1572962.
KJ891074 - Synthetic construct Homo sapiens clone ccsbBroadEn_00468 DUSP6 gene, encodes complete protein.
KJ896737 - Synthetic construct Homo sapiens clone ccsbBroadEn_06131 DUSP6 gene, encodes complete protein.
AB451265 - Homo sapiens DUSP6 mRNA for dual specificity phosphatase 6 isoform a, complete cds, clone: FLJ08085AAAN.
BT006895 - Homo sapiens dual specificity phosphatase 6 mRNA, complete cds.
DQ892490 - Synthetic construct clone IMAGE:100005120; FLH186777.01X; RZPDo839A0572D dual specificity phosphatase 6 (DUSP6) gene, encodes complete protein.
DQ895701 - Synthetic construct Homo sapiens clone IMAGE:100010161; FLH186773.01L; RZPDo839A0562D dual specificity phosphatase 6 (DUSP6) gene, encodes complete protein.
AB451393 - Homo sapiens DUSP6 mRNA for dual specificity phosphatase 6 isoform a, partial cds, clone: FLJ08085AAAF.
AB463588 - Synthetic construct DNA, clone: pF1KB6502, Homo sapiens DUSP6 gene for dual specificity phosphatase 6, without stop codon, in Flexi system.
CU678402 - Synthetic construct Homo sapiens gateway clone IMAGE:100019166 5' read DUSP6 mRNA.
DQ584050 - Homo sapiens piRNA piR-51162, complete sequence.
AB189400 - Homo sapiens DUSP6 mRNA for dual specificity phosphatase 6, partial cds.
JD024635 - Sequence 5659 from Patent EP1572962.
JD033401 - Sequence 14425 from Patent EP1572962.
JD293835 - Sequence 274859 from Patent EP1572962.
JD022496 - Sequence 3520 from Patent EP1572962.
JD035568 - Sequence 16592 from Patent EP1572962.
JD193175 - Sequence 174199 from Patent EP1572962.
DL492584 - Novel nucleic acids.
LY623322 - KR 1020180134347-A/9: DIAGNOSTIC AND THERAPEUTIC METHODS FOR CANCER.
MB416865 - JP 2019518426-A/5: DIAGNOSTIC AND THERAPEUTIC METHODS FOR CANCER.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_dspPathway - Regulation of MAP Kinase Pathways Through Dual Specificity Phosphatases

Reactome (by CSHL, EBI, and GO)

Protein Q16828 (Reactome details) participates in the following event(s):

R-HSA-203797 ERKs are inactivated by dual-specific phosphatases (DUSPs)
R-HSA-5675376 Cytosolic DUSPs dephosphorylate MAPKs
R-HSA-202670 ERKs are inactivated
R-HSA-112409 RAF-independent MAPK1/3 activation
R-HSA-5675221 Negative regulation of MAPK pathway
R-HSA-198753 ERK/MAPK targets
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-198725 Nuclear Events (kinase and transcription factor activation)
R-HSA-450282 MAPK targets/ Nuclear events mediated by MAP kinases
R-HSA-5683057 MAPK family signaling cascades
R-HSA-187037 Signaling by NTRK1 (TRKA)
R-HSA-450294 MAP kinase activation
R-HSA-162582 Signal Transduction
R-HSA-166520 Signaling by NTRKs
R-HSA-166058 MyD88:Mal cascade initiated on plasma membrane
R-HSA-168164 Toll Like Receptor 3 (TLR3) Cascade
R-HSA-448424 Interleukin-17 signaling
R-HSA-937061 TRIF(TICAM1)-mediated TLR4 signaling
R-HSA-975138 TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
R-HSA-975871 MyD88 cascade initiated on plasma membrane
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-166016 Toll Like Receptor 4 (TLR4) Cascade
R-HSA-168179 Toll Like Receptor TLR1:TLR2 Cascade
R-HSA-168188 Toll Like Receptor TLR6:TLR2 Cascade
R-HSA-168898 Toll-Like Receptors Cascades
R-HSA-449147 Signaling by Interleukins
R-HSA-166166 MyD88-independent TLR4 cascade
R-HSA-975155 MyD88 dependent cascade initiated on endosome
R-HSA-168142 Toll Like Receptor 10 (TLR10) Cascade
R-HSA-168176 Toll Like Receptor 5 (TLR5) Cascade
R-HSA-181438 Toll Like Receptor 2 (TLR2) Cascade
R-HSA-168249 Innate Immune System
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168138 Toll Like Receptor 9 (TLR9) Cascade
R-HSA-168181 Toll Like Receptor 7/8 (TLR7/8) Cascade
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: DUS6_HUMAN, ENST00000279488.1, ENST00000279488.2, ENST00000279488.3, ENST00000279488.4, ENST00000279488.5, ENST00000279488.6, ENST00000279488.7, MKP3, NM_001946, O75109, PYST1, Q16828, Q53Y75, Q9BSH6, uc317jsf.1, uc317jsf.2
UCSC ID: ENST00000279488.8_7
RefSeq Accession: NM_001946.4
Protein: Q16828 (aka DUS6_HUMAN)

-  GeneReviews for This Gene
  GeneReviews article(s) related to gene DUSP6:
kms (Isolated Gonadotropin-Releasing Hormone (GnRH) Deficiency)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.