Human Gene DZIP3 (ENST00000361582.8_4) from GENCODE V47lift37
  Description: DAZ interacting zinc finger protein 3 (from RefSeq NM_014648.4)
Gencode Transcript: ENST00000361582.8_4
Gencode Gene: ENSG00000198919.13_12
Transcript (Including UTRs)
   Position: hg19 chr3:108,308,569-108,413,687 Size: 105,119 Total Exon Count: 33 Strand: +
Coding Region
   Position: hg19 chr3:108,324,254-108,409,744 Size: 85,491 Coding Exon Count: 31 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:108,308,569-108,413,687)mRNA (may differ from genome)Protein (1208 aa)
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-  Comments and Description Text from UniProtKB
  ID: DZIP3_HUMAN
DESCRIPTION: RecName: Full=E3 ubiquitin-protein ligase DZIP3; EC=6.3.2.-; AltName: Full=DAZ-interacting protein 3; AltName: Full=RNA-binding ubiquitin ligase of 138 kDa; Short=hRUL138;
FUNCTION: E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with DAZ proteins.
INTERACTION: Q9H0E2:TOLLIP; NbExp=2; IntAct=EBI-948630, EBI-74615;
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Widely expressed at low level. Highly expressed in skeletal muscle, kidney and heart. Expressed at low level in placenta, lung, brain, liver and pancreas.
SIMILARITY: Contains 1 RING-type zinc finger.
SEQUENCE CAUTION: Sequence=AAH39018.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=AAH56674.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=AAK69484.1; Type=Frameshift; Positions=1127; Sequence=BAA31650.2; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 22.65 RPKM in Pituitary
Total median expression: 268.12 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -45.80190-0.241 Picture PostScript Text
3' UTR -356.501487-0.240 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001841 - Znf_RING
IPR013083 - Znf_RING/FYVE/PHD

Pfam Domains:
PF00097 - Zinc finger, C3HC4 type (RING finger)
PF12678 - RING-H2 zinc finger domain
PF13639 - Ring finger domain
PF13923 - Zinc finger, C3HC4 type (RING finger)
PF18738 - DZIP3/ hRUL138-like HEPN
PF19179 - Domain of unknown function (DUF5861)

SCOP Domains:
56954 - Outer membrane efflux proteins (OEP)
57850 - RING/U-box
57903 - FYVE/PHD zinc finger

ModBase Predicted Comparative 3D Structure on Q86Y13
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0019902 phosphatase binding
GO:0031593 polyubiquitin binding
GO:0046872 metal ion binding
GO:0051082 unfolded protein binding
GO:0061630 ubiquitin protein ligase activity
GO:1990381 ubiquitin-specific protease binding

Biological Process:
GO:0000209 protein polyubiquitination
GO:0016567 protein ubiquitination
GO:0030433 ER-associated ubiquitin-dependent protein catabolic process
GO:0030968 endoplasmic reticulum unfolded protein response

Cellular Component:
GO:0000836 Hrd1p ubiquitin ligase complex
GO:0005737 cytoplasm
GO:0036513 Derlin-1 retrotranslocation complex
GO:0044322 endoplasmic reticulum quality control compartment


-  Descriptions from all associated GenBank mRNAs
  AY227654 - Homo sapiens clone DKFZp586C1754Q3 RNA-binding RING-H2 protein-ubiquitin ligase short isoform mRNA, complete cds; alternatively spliced.
AK023138 - Homo sapiens cDNA FLJ13076 fis, clone NT2RP3001943, highly similar to Ubiquitin ligase protein DZIP3 (EC 6.3.2.-).
AK297451 - Homo sapiens cDNA FLJ58022 complete cds, highly similar to Ubiquitin ligase protein DZIP3 (EC 6.3.2.-).
BC063882 - Homo sapiens DAZ interacting protein 3, zinc finger, mRNA (cDNA clone MGC:75477 IMAGE:30387514), complete cds.
AB014575 - Homo sapiens KIAA0675 mRNA for KIAA0675 protein.
HQ258001 - Synthetic construct Homo sapiens clone IMAGE:100072310 DAZ interacting protein 3, zinc finger (DZIP3) (DZIP3) gene, encodes complete protein.
KJ892829 - Synthetic construct Homo sapiens clone ccsbBroadEn_02223 DZIP3 gene, encodes complete protein.
AB463003 - Synthetic construct DNA, clone: pF1KA0675, Homo sapiens DZIP3 gene for DAZ interacting protein 3, zinc finger, without stop codon, in Flexi system.
GQ129281 - Synthetic construct Homo sapiens clone HAIB:100068655; DKFZo004B0436 DAZ interacting protein 3, zinc finger protein (DZIP3) gene, partial cds.
GQ129282 - Synthetic construct Homo sapiens clone HAIB:100068559; DKFZo008B0435 DAZ interacting protein 3, zinc finger protein (DZIP3) gene, complete cds.
AK294967 - Homo sapiens cDNA FLJ57977 complete cds, highly similar to Ubiquitin ligase protein DZIP3 (EC 6.3.2.-).
AK303942 - Homo sapiens cDNA FLJ58223 complete cds, highly similar to Ubiquitin ligase protein DZIP3 (EC 6.3.2.-).
AY227652 - Homo sapiens clone ICRFp512H14173Q2 RNA-binding RING-H2 protein-ubiquitin ligase mRNA, partial cds.
AF279370 - Homo sapiens DZIP3 mRNA, partial cds.
AY495710 - Homo sapiens UURF2 ubiquitin ligase mRNA, partial cds.
AK023389 - Homo sapiens cDNA FLJ13327 fis, clone OVARC1001767, highly similar to Homo sapiens mRNA for KIAA0675 protein.
BC056674 - Homo sapiens DAZ interacting protein 3, zinc finger, mRNA (cDNA clone IMAGE:6617869), partial cds.
BC039018 - Homo sapiens DAZ interacting protein 3, zinc finger, mRNA (cDNA clone IMAGE:6046587), partial cds.
AY227651 - Homo sapiens clone NIII RNA-binding RING-H2 protein-ubiquitin ligase mRNA, partial cds.
BC012631 - Homo sapiens zinc finger DAZ interacting protein 3, mRNA (cDNA clone IMAGE:4096020), with apparent retained intron.
JD079248 - Sequence 60272 from Patent EP1572962.
AY227653 - Homo sapiens clone ICRFp512B2269Q2 RNA-binding RING-H2 protein-ubiquitin ligase precursor RNA, partial cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q86Y13 (Reactome details) participates in the following event(s):

R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-983147 Release of E3 from polyubiquitinated substrate
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: B3KN01, DZIP3_HUMAN, ENST00000361582.1, ENST00000361582.2, ENST00000361582.3, ENST00000361582.4, ENST00000361582.5, ENST00000361582.6, ENST00000361582.7, KIAA0675, NM_014648, O75162, Q6P3R9, Q6PH82, Q86Y13, Q86Y14, Q86Y15, Q86Y16, Q8IWI0, Q96RS9, uc318cer.1, uc318cer.2
UCSC ID: ENST00000361582.8_4
RefSeq Accession: NM_014648.4
Protein: Q86Y13 (aka DZIP3_HUMAN or DZP3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.