Human Gene E2F8 (ENST00000250024.9_7) from GENCODE V47lift37
  Description: E2F transcription factor 8, transcript variant 1 (from RefSeq NM_024680.4)
Gencode Transcript: ENST00000250024.9_7
Gencode Gene: ENSG00000129173.13_9
Transcript (Including UTRs)
   Position: hg19 chr11:19,245,610-19,262,492 Size: 16,883 Total Exon Count: 13 Strand: -
Coding Region
   Position: hg19 chr11:19,246,205-19,261,668 Size: 15,464 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:19,245,610-19,262,492)mRNA (may differ from genome)Protein (867 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: E2F8_HUMAN
DESCRIPTION: RecName: Full=Transcription factor E2F8; Short=E2F-8;
FUNCTION: Along with E2F7, inhibitor of E2F-dependent transcription. Binds DNA independently of DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Directly represses E2F1 transcription. Appears to regulate a subset of E2F-dependent genes whose products are required for normal cell cycle progession.
SUBUNIT: Forms heterodimers with E2F7 and, to a lesser extent, homodimers. Dimerization is important for DNA binding.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: Both DNA-binding domains are required for DNA-binding and are proposed to form an intramolecular structure that is similar to the winged helix structure of the E2F-DP heterodimer (By similarity).
SIMILARITY: Belongs to the E2F/DP family.
SEQUENCE CAUTION: Sequence=AAH28244.2; Type=Erroneous initiation; Sequence=BAB15605.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 6.80 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 23.13 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -145.60507-0.287 Picture PostScript Text
3' UTR -113.24595-0.190 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR015633 - E2F
IPR003316 - E2F_TDP
IPR011991 - WHTH_trsnscrt_rep_DNA-bd

Pfam Domains:
PF02319 - E2F/DP family winged-helix DNA-binding domain

SCOP Domains:
46785 - "Winged helix" DNA-binding domain

ModBase Predicted Comparative 3D Structure on A0AVK6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001047 core promoter binding
GO:0001078 transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0003714 transcription corepressor activity
GO:0005515 protein binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001890 placenta development
GO:0002040 sprouting angiogenesis
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0007049 cell cycle
GO:0008283 cell proliferation
GO:0032466 negative regulation of cytokinesis
GO:0032877 positive regulation of DNA endoreduplication
GO:0033301 cell cycle comprising mitosis without cytokinesis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0060707 trophoblast giant cell differentiation
GO:0060718 chorionic trophoblast cell differentiation
GO:0070365 hepatocyte differentiation

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005667 transcription factor complex
GO:0005730 nucleolus
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AK026964 - Homo sapiens cDNA: FLJ23311 fis, clone HEP11681.
BC090877 - Homo sapiens E2F transcription factor 8, mRNA (cDNA clone IMAGE:30383200), partial cds.
AK055206 - Homo sapiens cDNA FLJ30644 fis, clone CTONG2003945, weakly similar to TRANSCRIPTION FACTOR E2F3.
AK292688 - Homo sapiens cDNA FLJ76958 complete cds, highly similar to Homo sapiens E2F transcription factor 8 (E2F8), mRNA.
JD337378 - Sequence 318402 from Patent EP1572962.
BC028244 - Homo sapiens E2F transcription factor 8, mRNA (cDNA clone IMAGE:5406977), partial cds.
BC126402 - Homo sapiens E2F transcription factor 8, mRNA (cDNA clone MGC:161680 IMAGE:8992118), complete cds.
BC126400 - Homo sapiens E2F transcription factor 8, mRNA (cDNA clone MGC:161678 IMAGE:8992116), complete cds.
AK225338 - Homo sapiens mRNA for E2F family member 8 variant, clone: HEP11681.
KJ903211 - Synthetic construct Homo sapiens clone ccsbBroadEn_12605 E2F8 gene, encodes complete protein.
HQ258679 - Synthetic construct Homo sapiens clone IMAGE:100072709 E2F transcription factor 8 (E2F8) (E2F8) gene, encodes complete protein.
KJ899558 - Synthetic construct Homo sapiens clone ccsbBroadEn_08952 E2F8 gene, encodes complete protein.
CU688446 - Synthetic construct Homo sapiens gateway clone IMAGE:100019364 5' read E2F8 mRNA.
JD192243 - Sequence 173267 from Patent EP1572962.
JD042163 - Sequence 23187 from Patent EP1572962.
JD322356 - Sequence 303380 from Patent EP1572962.
JD094263 - Sequence 75287 from Patent EP1572962.
JD450446 - Sequence 431470 from Patent EP1572962.
AB231783 - Homo sapiens mRNA for hypothetical protein, partial sequence, clone:Hsa11-digit34-10-01-R.
AB231782 - Homo sapiens mRNA for hypothetical protein, partial sequence, clone:Hsa11-digit34-09-16-R.
BC108700 - Homo sapiens E2F transcription factor 8, mRNA (cDNA clone IMAGE:6065043), partial cds.
AB231781 - Homo sapiens mRNA for hypothetical protein, partial cds, clone:Hsa11-digit34-09-16-F.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein A0AVK6 (Reactome details) participates in the following event(s):

R-HSA-8953013 E2F7 binds E2F8
R-HSA-8953037 E2F8 forms homodimers
R-HSA-6798347 E2F7 and E2F8 homo- and heterodimers bind the E2F1 gene promoter
R-HSA-6804116 TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest
R-HSA-6791312 TP53 Regulates Transcription of Cell Cycle Genes
R-HSA-3700989 Transcriptional Regulation by TP53
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: A0AVK6, A8K9H3, E2F8_HUMAN, ENST00000250024.1, ENST00000250024.2, ENST00000250024.3, ENST00000250024.4, ENST00000250024.5, ENST00000250024.6, ENST00000250024.7, ENST00000250024.8, NM_024680, Q2VPJ3, Q3C1U6, Q5BKY4, Q8N340, Q9H5M0, uc317fap.1, uc317fap.2
UCSC ID: ENST00000250024.9_7
RefSeq Accession: NM_024680.4
Protein: A0AVK6 (aka E2F8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.