Human Gene EEF1B2 (ENST00000392222.7_4) from GENCODE V47lift37
  Description: eukaryotic translation elongation factor 1 beta 2, transcript variant 1 (from RefSeq NM_001959.4)
Gencode Transcript: ENST00000392222.7_4
Gencode Gene: ENSG00000114942.15_10
Transcript (Including UTRs)
   Position: hg19 chr2:207,024,619-207,027,652 Size: 3,034 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg19 chr2:207,024,704-207,027,607 Size: 2,904 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:207,024,619-207,027,652)mRNA (may differ from genome)Protein (225 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: EF1B_HUMAN
DESCRIPTION: RecName: Full=Elongation factor 1-beta; Short=EF-1-beta;
FUNCTION: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.
SUBUNIT: EF-1 is composed of 4 subunits: alpha, beta, delta, and gamma.
INTERACTION: P26641:EEF1G; NbExp=3; IntAct=EBI-354334, EBI-351467;
PTM: Phosphorylation affects the GDP/GTP exchange rate.
SIMILARITY: Belongs to the EF-1-beta/EF-1-delta family.
SIMILARITY: Contains 1 GST C-terminal domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 586.47 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 10953.12 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -19.2085-0.226 Picture PostScript Text
3' UTR -3.2045-0.071 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018940 - EF-1_beta_acid_region_euk
IPR010987 - Glutathione-S-Trfase_C-like
IPR014717 - Transl_elong_EF1B/ribosomal_S6
IPR001326 - Transl_elong_EF1B_B/D_CS
IPR014038 - Transl_elong_fac_EF1B_bsu/dsu

Pfam Domains:
PF00736 - EF-1 guanine nucleotide exchange domain
PF10587 - Eukaryotic elongation factor 1 beta central acidic region

SCOP Domains:
47616 - GST C-terminal domain-like
48097 - Regulator of G-protein signaling, RGS
54984 - eEF-1beta-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1B64 - NMR MuPIT


ModBase Predicted Comparative 3D Structure on P24534
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene DetailsGene Details Gene Details Gene Details
Gene SorterGene Sorter Gene Sorter Gene Sorter
 RGDEnsembl  SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003746 translation elongation factor activity
GO:0005515 protein binding

Biological Process:
GO:0006412 translation
GO:0006414 translational elongation
GO:0045471 response to ethanol

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005853 eukaryotic translation elongation factor 1 complex
GO:0005783 endoplasmic reticulum
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  S81522 - elongation factor 1-beta {5' region, clone CEM1002} [human, lymphoblastoid cell line CEM, mRNA Partial, 513 nt].
X60656 - H.sapiens mRNA for elongation factor 1-beta.
BC000211 - Homo sapiens eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:2349 IMAGE:3353094), complete cds.
X60489 - Human mRNA for elongation factor-1-beta.
D28350 - Homo sapiens mRNA for elongation factor 1-beta, 5'UTR region.
AK310096 - Homo sapiens cDNA, FLJ17138.
AK291910 - Homo sapiens cDNA FLJ75751 complete cds, highly similar to Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 1, mRNA.
BC067787 - Homo sapiens eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:87191 IMAGE:5299844), complete cds.
BC029316 - Homo sapiens eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone IMAGE:3462876).
BC004931 - Homo sapiens eukaryotic translation elongation factor 1 beta 2, mRNA (cDNA clone MGC:10551 IMAGE:3611581), complete cds.
JD271637 - Sequence 252661 from Patent EP1572962.
AB529032 - Synthetic construct DNA, clone: pF1KB3431, Homo sapiens EEF1B2 gene for eukaryotic translation elongation factor 1 beta 2, without stop codon, in Flexi system.
CU688340 - Synthetic construct Homo sapiens gateway clone IMAGE:100022905 5' read EEF1B2 mRNA.
JF432608 - Synthetic construct Homo sapiens clone IMAGE:100073845 eukaryotic translation elongation factor 1 beta 2 (EEF1B2) gene, encodes complete protein.
KJ891088 - Synthetic construct Homo sapiens clone ccsbBroadEn_00482 EEF1B2 gene, encodes complete protein.
KJ905733 - Synthetic construct Homo sapiens clone ccsbBroadEn_15403 EEF1B2 gene, encodes complete protein.
BT007079 - Homo sapiens eukaryotic translation elongation factor 1 beta 2 mRNA, complete cds.
CR456825 - Homo sapiens full open reading frame cDNA clone RZPDo834E0315D for gene EEF1B2, eukaryotic translation elongation factor 1 beta 2; complete cds, incl. stopcodon.
DQ588028 - Homo sapiens piRNA piR-55140, complete sequence.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P24534 (Reactome details) participates in the following event(s):

R-HSA-156910 Formation of eEF1B complex
R-HSA-156913 Regeneration of eEF1A:GTP by eEF1B activity
R-HSA-156842 Eukaryotic Translation Elongation
R-HSA-72766 Translation
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: A8K795, EEF1B, EF1B, EF1B_HUMAN, ENST00000392222.1, ENST00000392222.2, ENST00000392222.3, ENST00000392222.4, ENST00000392222.5, ENST00000392222.6, NM_001959, P24534, Q6IBH9, uc318uxu.1, uc318uxu.2
UCSC ID: ENST00000392222.7_4
RefSeq Accession: NM_001959.4
Protein: P24534 (aka EF1B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.