Human Gene EIF2A (ENST00000460851.6_10) from GENCODE V47lift37
  Description: eukaryotic translation initiation factor 2A, transcript variant 1 (from RefSeq NM_032025.5)
Gencode Transcript: ENST00000460851.6_10
Gencode Gene: ENSG00000144895.12_15
Transcript (Including UTRs)
   Position: hg19 chr3:150,264,574-150,303,803 Size: 39,230 Total Exon Count: 14 Strand: +
Coding Region
   Position: hg19 chr3:150,264,590-150,301,698 Size: 37,109 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:150,264,574-150,303,803)mRNA (may differ from genome)Protein (585 aa)
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: EIF2A_HUMAN
DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 2A; Short=eIF-2A; AltName: Full=65 kDa eukaryotic translation initiation factor 2A;
FUNCTION: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
TISSUE SPECIFICITY: Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.
SIMILARITY: Belongs to the WD repeat EIF2A family.
SIMILARITY: Contains 3 WD repeats.
CAUTION: Was initially thought to constitute the ortholog of prokaryotic IF-2 (infB) protein.
SEQUENCE CAUTION: Sequence=AAK14926.1; Type=Erroneous initiation; Sequence=AAM83402.1; Type=Frameshift; Positions=82, 90; Sequence=AAQ13506.1; Type=Frameshift; Positions=513; Sequence=AAQ13612.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 77.91 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 1534.95 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -0.4016-0.025 Picture PostScript Text
3' UTR -572.102105-0.272 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011387 - TIF2A
IPR013979 - TIF2A_beta_prop-like
IPR015943 - WD40/YVTN_repeat-like_dom

Pfam Domains:
PF08662 - Eukaryotic translation initiation factor eIF2A

SCOP Domains:
50952 - Soluble quinoprotein glucose dehydrogenase
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
117289 - Nucleoporin domain
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Peptidase/esterase 'gauge' domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - DPP6 N-terminal domain-like

ModBase Predicted Comparative 3D Structure on Q9BY44
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000049 tRNA binding
GO:0003729 mRNA binding
GO:0003743 translation initiation factor activity
GO:0005515 protein binding
GO:0043022 ribosome binding
GO:0045296 cadherin binding

Biological Process:
GO:0006412 translation
GO:0006413 translational initiation
GO:0006417 regulation of translation
GO:0006468 protein phosphorylation
GO:0009967 positive regulation of signal transduction
GO:0032933 SREBP signaling pathway
GO:0042255 ribosome assembly
GO:1990928 response to amino acid starvation

Cellular Component:
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0005850 eukaryotic translation initiation factor 2 complex
GO:0022627 cytosolic small ribosomal subunit
GO:0072562 blood microparticle


-  Descriptions from all associated GenBank mRNAs
  AF212241 - Homo sapiens CDA02 mRNA, complete cds.
AK298586 - Homo sapiens cDNA FLJ58035 complete cds, highly similar to Homo sapiens eukaryotic translation initiation factor (eIF) 2A (eIF2A), mRNA.
AK293993 - Homo sapiens cDNA FLJ57962 complete cds, highly similar to Homo sapiens eukaryotic translation initiation factor (eIF) 2A (eIF2A), mRNA.
AK027356 - Homo sapiens cDNA FLJ14450 fis, clone HEMBB1001736, weakly similar to EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 9.
BX647951 - Homo sapiens mRNA; cDNA DKFZp686F2451 (from clone DKFZp686F2451).
AL833101 - Homo sapiens mRNA; cDNA DKFZp313E0915 (from clone DKFZp313E0915).
BC011885 - Homo sapiens eukaryotic translation initiation factor 2A, 65kDa, mRNA (cDNA clone MGC:20557 IMAGE:3688407), complete cds.
AF497978 - Homo sapiens eukaryotic translation initiation factor 2A mRNA, complete cds.
AB590213 - Synthetic construct DNA, clone: pFN21AE1363, Homo sapiens EIF2A gene for eukaryotic translation initiation factor 2A, 65kDa, without stop codon, in Flexi system.
DQ896788 - Synthetic construct Homo sapiens clone IMAGE:100011248; FLH199407.01L; RZPDo839E0981D eukaryotic translation initiation factor 2A, 65kDa (EIF2A) gene, encodes complete protein.
EU176412 - Synthetic construct Homo sapiens clone IMAGE:100006536; FLH197109.01X; RZPDo839C05252D eukaryotic translation initiation factor 2A, 65kDa (EIF2A) gene, encodes complete protein.
AF172818 - Homo sapiens MSTP089 (MST089) mRNA, complete cds.
AF109358 - Homo sapiens MSTP004 mRNA, complete cds.
JD357562 - Sequence 338586 from Patent EP1572962.
JD312741 - Sequence 293765 from Patent EP1572962.
JD225946 - Sequence 206970 from Patent EP1572962.
JD171398 - Sequence 152422 from Patent EP1572962.
JD197778 - Sequence 178802 from Patent EP1572962.
JD412734 - Sequence 393758 from Patent EP1572962.
JD512688 - Sequence 493712 from Patent EP1572962.
JD340672 - Sequence 321696 from Patent EP1572962.
JD499634 - Sequence 480658 from Patent EP1572962.
JD286634 - Sequence 267658 from Patent EP1572962.
JD511477 - Sequence 492501 from Patent EP1572962.
JD564345 - Sequence 545369 from Patent EP1572962.
JD558275 - Sequence 539299 from Patent EP1572962.
JD319354 - Sequence 300378 from Patent EP1572962.
JD223320 - Sequence 204344 from Patent EP1572962.
JD225092 - Sequence 206116 from Patent EP1572962.
JD506403 - Sequence 487427 from Patent EP1572962.
JD061174 - Sequence 42198 from Patent EP1572962.
JD162366 - Sequence 143390 from Patent EP1572962.
JD282061 - Sequence 263085 from Patent EP1572962.
JD230257 - Sequence 211281 from Patent EP1572962.
JD270176 - Sequence 251200 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_chemicalPathway - Apoptotic Signaling in Response to DNA Damage
h_igf1mtorpathway - Skeletal muscle hypertrophy is regulated via AKT/mTOR pathway
h_eifPathway - Eukaryotic protein translation
h_rnaPathway - Double Stranded RNA Induced Gene Expression
h_vegfPathway - VEGF, Hypoxia, and Angiogenesis
h_eif2Pathway - Regulation of eIF2

-  Other Names for This Gene
  Alternate Gene Symbols: A8MPS6, B4DF96, B4DQ14, CDA02, D3DNI9, EIF2A_HUMAN, ENST00000460851.1, ENST00000460851.2, ENST00000460851.3, ENST00000460851.4, ENST00000460851.5, MSTP004, MSTP089, NM_032025, Q5QTR2, Q7Z4E9, Q8NFM1, Q96EW9, Q96K81, Q9BY44, uc321cbv.1, uc321cbv.2
UCSC ID: ENST00000460851.6_10
RefSeq Accession: NM_032025.5
Protein: Q9BY44 (aka EIF2A_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.