ID:EIF3F_HUMAN DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 3 subunit F; Short=eIF3f; AltName: Full=Deubiquitinating enzyme eIF3f; EC=3.4.19.12; AltName: Full=Eukaryotic translation initiation factor 3 subunit 5; AltName: Full=eIF-3-epsilon; AltName: Full=eIF3 p47; FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. FUNCTION: Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. CATALYTIC ACTIVITY: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C- terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). SUBUNIT: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination- dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1. INTERACTION: Q9Q2G4:ORF (xeno); NbExp=3; IntAct=EBI-711990, EBI-6248094; SUBCELLULAR LOCATION: Cytoplasm (By similarity). DOMAIN: The MPN domain mediates deubiquitinating activity. PTM: Phosphorylated. Phosphorylation is enhanced upon serum stimulation. MASS SPECTROMETRY: Mass=37554.8; Method=Unknown; Range=1-357; Source=PubMed:17322308; MASS SPECTROMETRY: Mass=37475.6; Mass_error=0.2; Method=MALDI; Range=1-357; Source=PubMed:18599441; SIMILARITY: Belongs to the eIF-3 subunit F family. SIMILARITY: Contains 1 MPN (JAB/Mov34) domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF01398 - JAB1/Mov34/MPN/PAD-1 ubiquitin protease PF13012 - Maintenance of mitochondrial structure and function
ModBase Predicted Comparative 3D Structure on O00303
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK093511 - Homo sapiens cDNA FLJ36192 fis, clone TESTI2027450, highly similar to Eukaryotic translation initiation factor 3 subunit 5. AK289637 - Homo sapiens cDNA FLJ75282 complete cds, highly similar to Homo sapiens eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa (EIF3S5), mRNA. AK297621 - Homo sapiens cDNA FLJ52696 complete cds, highly similar to Eukaryotic translation initiation factor 3 subunit 5. AK095574 - Homo sapiens cDNA FLJ38255 fis, clone FCBBF3001020, highly similar to EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT 5. AK291354 - Homo sapiens cDNA FLJ78396 complete cds, highly similar to Homo sapiens eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa (EIF3S5), mRNA. AK293826 - Homo sapiens cDNA FLJ52478 complete cds, highly similar to Eukaryotic translation initiation factor 3 subunit 5. U94855 - Homo sapiens translation initiation factor 3 47 kDa subunit mRNA, complete cds. BC000490 - Homo sapiens eukaryotic translation initiation factor 3, subunit F, mRNA (cDNA clone MGC:8365 IMAGE:2819946), complete cds. BC073753 - Homo sapiens eukaryotic translation initiation factor 3, subunit F, mRNA (cDNA clone IMAGE:6424468). CU674238 - Synthetic construct Homo sapiens gateway clone IMAGE:100017090 5' read EIF3F mRNA. CR456959 - Homo sapiens full open reading frame cDNA clone RZPDo834B0519D for gene EIF3S5, eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; complete cds, incl. stopcodon. AB590115 - Synthetic construct DNA, clone: pFN21AE1309, Homo sapiens EIF3F gene for eukaryotic translation initiation factor 3, subunit F, without stop codon, in Flexi system. BT006894 - Homo sapiens eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa mRNA, complete cds. DQ890766 - Synthetic construct clone IMAGE:100003396; FLH165782.01X; RZPDo839G08160D eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa (EIF3S5) gene, encodes complete protein. HQ448263 - Synthetic construct Homo sapiens clone IMAGE:100071665; CCSB003148_01 eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa (EIF3F) gene, encodes complete protein. KJ897888 - Synthetic construct Homo sapiens clone ccsbBroadEn_07282 EIF3F gene, encodes complete protein. AK027739 - Homo sapiens cDNA FLJ14833 fis, clone OVARC1001171, moderately similar to Homo sapiens translation initiation factor 3 47 kDa subunit mRNA. DQ584764 - Homo sapiens piRNA piR-51876, complete sequence. DQ601912 - Homo sapiens piRNA piR-39978, complete sequence. AK057956 - Homo sapiens cDNA FLJ25227 fis, clone STM01429. JD217794 - Sequence 198818 from Patent EP1572962. JD283288 - Sequence 264312 from Patent EP1572962. JD496993 - Sequence 478017 from Patent EP1572962. JD135689 - Sequence 116713 from Patent EP1572962. JD238033 - Sequence 219057 from Patent EP1572962. JD047484 - Sequence 28508 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein O00303 (Reactome details) participates in the following event(s):
R-HSA-72676 eIF3 and eIF1A bind to the 40S subunit R-HSA-72619 eIF2:GTP is hydrolyzed, eIFs are released R-HSA-72691 Formation of the 43S pre-initiation complex R-HSA-156808 Formation of translation initiation complexes yielding circularized Ceruloplasmin mRNA in a 'closed-loop' conformation R-HSA-157849 Formation of translation initiation complexes containing mRNA that does not circularize R-HSA-72621 Ribosomal scanning R-HSA-72697 Start codon recognition R-HSA-156823 Association of phospho-L13a with GAIT element of Ceruloplasmin mRNA R-HSA-72689 Formation of a pool of free 40S subunits R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex R-HSA-72649 Translation initiation complex formation R-HSA-72737 Cap-dependent Translation Initiation R-HSA-72702 Ribosomal scanning and start codon recognition R-HSA-156827 L13a-mediated translation R-HSA-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-HSA-72613 Eukaryotic Translation Initiation R-HSA-72766 Translation R-HSA-392499 Metabolism of proteins
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
