ID:EIF3G_HUMAN DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 3 subunit G; Short=eIF3g; AltName: Full=Eukaryotic translation initiation factor 3 RNA-binding subunit; Short=eIF-3 RNA-binding subunit; AltName: Full=Eukaryotic translation initiation factor 3 subunit 4; AltName: Full=eIF-3-delta; AltName: Full=eIF3 p42; AltName: Full=eIF3 p44; FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA. SUBUNIT: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts (via C-terminus) with AIFM1 (via N-terminus). INTERACTION: P55884:EIF3B; NbExp=4; IntAct=EBI-366632, EBI-366696; SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus. Cytoplasm, perinuclear region. Note=Colocalizes with AIFM1 in the nucleus and perinuclear region. PTM: Phosphorylated. Phosphorylation is enhanced upon serum stimulation. MASS SPECTROMETRY: Mass=35639.8; Method=Unknown; Range=1-320; Source=PubMed:17322308; MASS SPECTROMETRY: Mass=35481.1; Mass_error=0.4; Method=MALDI; Range=1-320; Source=PubMed:18599441; SIMILARITY: Belongs to the eIF-3 subunit G family. SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O75821
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AF094850 - Homo sapiens eukaryotic translation initiation factor 3 subunit p42/p44 mRNA, complete cds. BC008469 - Homo sapiens eukaryotic translation initiation factor 3, subunit G, mRNA (cDNA clone MGC:14741 IMAGE:4279770), complete cds. AF020833 - Homo sapiens eukaryotic translation initiation factor 3 subunit (p42) mRNA, complete cds. U96074 - Human translation initiation factor eIF3 p44 subunit mRNA, complete cds. AK291320 - Homo sapiens cDNA FLJ76297 complete cds, highly similar to Homo sapiens eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa (EIF3S4), mRNA. BC000733 - Homo sapiens eukaryotic translation initiation factor 3, subunit G, mRNA (cDNA clone MGC:2053 IMAGE:3504640), complete cds. BT006889 - Homo sapiens eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa mRNA, complete cds. DQ890616 - Synthetic construct clone IMAGE:100003246; FLH164378.01X; RZPDo839F08162D eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa (EIF3S4) gene, encodes complete protein. KJ892590 - Synthetic construct Homo sapiens clone ccsbBroadEn_01984 EIF3G gene, encodes complete protein. KJ897889 - Synthetic construct Homo sapiens clone ccsbBroadEn_07283 EIF3G gene, encodes complete protein. DQ896801 - Synthetic construct Homo sapiens clone IMAGE:100011261; FLH164374.01L; RZPDo839F08161D eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa (EIF3S4) gene, encodes complete protein. AB528489 - Synthetic construct DNA, clone: pF1KE0745, Homo sapiens EIF3G gene for eukaryotic translation initiation factor 3, subunit G, without stop codon, in Flexi system. CR457134 - Homo sapiens full open reading frame cDNA clone RZPDo834A0412D for gene EIF3S4, eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa; complete cds, incl. stopcodon. JD035942 - Sequence 16966 from Patent EP1572962. AK316335 - Homo sapiens cDNA, FLJ79234 complete cds, moderately similar to Eukaryotic translation initiation factor 3 subunit 4. AK293374 - Homo sapiens cDNA FLJ51582 complete cds, moderately similar to Eukaryotic translation initiation factor 3 subunit 4. AK316040 - Homo sapiens cDNA, FLJ78939 complete cds, moderately similar to Eukaryotic translation initiation factor 3 subunit 4. CU678971 - Synthetic construct Homo sapiens gateway clone IMAGE:100017023 5' read EIF3G mRNA. AK315896 - Homo sapiens cDNA, FLJ79545 complete cds, highly similar to Eukaryotic translation initiation factor 3subunit 4. AK309944 - Homo sapiens cDNA, FLJ99985. AK311624 - Homo sapiens cDNA, FLJ18666. AK296377 - Homo sapiens cDNA FLJ59915 complete cds.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein O75821 (Reactome details) participates in the following event(s):
R-HSA-72676 eIF3 and eIF1A bind to the 40S subunit R-HSA-72619 eIF2:GTP is hydrolyzed, eIFs are released R-HSA-72691 Formation of the 43S pre-initiation complex R-HSA-156808 Formation of translation initiation complexes yielding circularized Ceruloplasmin mRNA in a 'closed-loop' conformation R-HSA-157849 Formation of translation initiation complexes containing mRNA that does not circularize R-HSA-72621 Ribosomal scanning R-HSA-72697 Start codon recognition R-HSA-156823 Association of phospho-L13a with GAIT element of Ceruloplasmin mRNA R-HSA-72689 Formation of a pool of free 40S subunits R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex R-HSA-72649 Translation initiation complex formation R-HSA-72737 Cap-dependent Translation Initiation R-HSA-72702 Ribosomal scanning and start codon recognition R-HSA-156827 L13a-mediated translation R-HSA-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-HSA-72613 Eukaryotic Translation Initiation R-HSA-72766 Translation R-HSA-392499 Metabolism of proteins
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
