ID:EIF3M_HUMAN DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 3 subunit M; Short=eIF3m; AltName: Full=Fetal lung protein B5; Short=hFL-B5; AltName: Full=PCI domain-containing protein 1; FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2). SUBUNIT: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. INTERACTION: Q9Q2G4:ORF (xeno); NbExp=2; IntAct=EBI-353901, EBI-6248094; SUBCELLULAR LOCATION: Cytoplasm (By similarity). TISSUE SPECIFICITY: Broadly expressed. INDUCTION: By glucose deprivation in neuroblastoma cells. MASS SPECTROMETRY: Mass=42413.8; Method=Unknown; Range=1-374; Source=PubMed:17322308; MASS SPECTROMETRY: Mass=42414.7; Mass_error=0.2; Method=MALDI; Range=1-374; Source=PubMed:18599441; SIMILARITY: Belongs to the eIF-3 subunit M family. SIMILARITY: Contains 1 PCI domain. SEQUENCE CAUTION: Sequence=AAK07542.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAK07542.1; Type=Frameshift; Positions=371;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
SCOP Domains: 48371 - ARM repeat 46785 - "Winged helix" DNA-binding domain 47986 - DEATH domain 51569 - Aldolase
ModBase Predicted Comparative 3D Structure on Q7L2H7
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
KJ893056 - Synthetic construct Homo sapiens clone ccsbBroadEn_02450 EIF3M gene, encodes complete protein. LF205808 - JP 2014500723-A/13311: Polycomb-Associated Non-Coding RNAs. AK312512 - Homo sapiens cDNA, FLJ92877, Homo sapiens dendritic cell protein (GA17), mRNA. LF205155 - JP 2014500723-A/12658: Polycomb-Associated Non-Coding RNAs. AK131064 - Homo sapiens cDNA FLJ29030 fis, clone SPL02144, highly similar to Homo sapiens dendritic cell protein (GA17). LP895217 - Sequence 81 from Patent EP3253886. AK292139 - Homo sapiens cDNA FLJ78709 complete cds, highly similar to Homo sapiens dendritic cell protein (GA17), mRNA. AK304378 - Homo sapiens cDNA FLJ54904 complete cds, highly similar to Homo sapiens dendritic cell protein (hfl-B5), mRNA. AK300002 - Homo sapiens cDNA FLJ60145 complete cds, highly similar to Homo sapiens dendritic cell protein (hfl-B5), mRNA. AK001768 - Homo sapiens cDNA FLJ10906 fis, clone OVARC1000035. AF064603 - Homo sapiens GA17 protein mRNA, complete cds. BC051292 - Homo sapiens eukaryotic translation initiation factor 3, subunit M, mRNA (cDNA clone MGC:59635 IMAGE:3607828), complete cds. BC011937 - Homo sapiens cDNA clone IMAGE:4026267, containing frame-shift errors. BC019103 - Homo sapiens eukaryotic translation initiation factor 3, subunit M, mRNA (cDNA clone MGC:29604 IMAGE:5021401), complete cds. AK307121 - Homo sapiens cDNA, FLJ97069. AK222564 - Homo sapiens mRNA for dendritic cell protein variant, clone: CAE03638. CU676384 - Synthetic construct Homo sapiens gateway clone IMAGE:100023397 5' read EIF3M mRNA. CR450300 - Homo sapiens full open reading frame cDNA clone RZPDo834D011D for gene GA17, dendritic cell protein; complete cds; without stopcodon. AY769947 - Homo sapiens fetal lung protein B5 (GA17) mRNA, complete cds. DQ185042 - Homo sapiens dendritic cell protein GA17 mRNA, partial cds. DL492224 - Novel nucleic acids. LF348446 - JP 2014500723-A/155949: Polycomb-Associated Non-Coding RNAs. LF317140 - JP 2014500723-A/124643: Polycomb-Associated Non-Coding RNAs. JD035003 - Sequence 16027 from Patent EP1572962. BC020458 - Homo sapiens eukaryotic translation initiation factor 3, subunit M, mRNA (cDNA clone IMAGE:3856311). AF277183 - Homo sapiens PNAS-125 mRNA, complete cds. LF317139 - JP 2014500723-A/124642: Polycomb-Associated Non-Coding RNAs. LF317138 - JP 2014500723-A/124641: Polycomb-Associated Non-Coding RNAs. LF317137 - JP 2014500723-A/124640: Polycomb-Associated Non-Coding RNAs. AK123310 - Homo sapiens cDNA FLJ41316 fis, clone BRAMY2043314. JD388338 - Sequence 369362 from Patent EP1572962. JD224260 - Sequence 205284 from Patent EP1572962. JD342205 - Sequence 323229 from Patent EP1572962. JD488146 - Sequence 469170 from Patent EP1572962. MA440732 - JP 2018138019-A/12658: Polycomb-Associated Non-Coding RNAs. MA584023 - JP 2018138019-A/155949: Polycomb-Associated Non-Coding RNAs. MA441385 - JP 2018138019-A/13311: Polycomb-Associated Non-Coding RNAs. MA552717 - JP 2018138019-A/124643: Polycomb-Associated Non-Coding RNAs. MA552716 - JP 2018138019-A/124642: Polycomb-Associated Non-Coding RNAs. MA552715 - JP 2018138019-A/124641: Polycomb-Associated Non-Coding RNAs. MA552714 - JP 2018138019-A/124640: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q7L2H7 (Reactome details) participates in the following event(s):
R-HSA-72676 eIF3 and eIF1A bind to the 40S subunit R-HSA-72619 eIF2:GTP is hydrolyzed, eIFs are released R-HSA-72691 Formation of the 43S pre-initiation complex R-HSA-156808 Formation of translation initiation complexes yielding circularized Ceruloplasmin mRNA in a 'closed-loop' conformation R-HSA-157849 Formation of translation initiation complexes containing mRNA that does not circularize R-HSA-72621 Ribosomal scanning R-HSA-72697 Start codon recognition R-HSA-156823 Association of phospho-L13a with GAIT element of Ceruloplasmin mRNA R-HSA-72689 Formation of a pool of free 40S subunits R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex R-HSA-72649 Translation initiation complex formation R-HSA-72737 Cap-dependent Translation Initiation R-HSA-72702 Ribosomal scanning and start codon recognition R-HSA-156827 L13a-mediated translation R-HSA-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-HSA-72613 Eukaryotic Translation Initiation R-HSA-72766 Translation R-HSA-392499 Metabolism of proteins
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
