Human Gene EIF5AL1 (ENST00000520547.4_4) from GENCODE V47lift37
  Description: eukaryotic translation initiation factor 5A like 1 (from RefSeq NM_001099692.2)
Gencode Transcript: ENST00000520547.4_4
Gencode Gene: ENSG00000253626.4_8
Transcript (Including UTRs)
   Position: hg19 chr10:81,272,289-81,276,196 Size: 3,908 Total Exon Count: 1 Strand: +
Coding Region
   Position: hg19 chr10:81,272,406-81,272,870 Size: 465 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Model InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:81,272,289-81,276,196)mRNA (may differ from genome)Protein (154 aa)
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-  Comments and Description Text from UniProtKB
  ID: IF5AL_HUMAN
DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 5A-1-like; Short=eIF-5A-1-like; Short=eIF-5A1-like; AltName: Full=Eukaryotic initiation factor 5A isoform 1-like;
FUNCTION: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Nucleus, nuclear pore complex (By similarity). Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions (By similarity).
PTM: eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine) (By similarity).
SIMILARITY: Belongs to the eIF-5A family.
SEQUENCE CAUTION: Sequence=AAH70048.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.45 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 231.29 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -54.30117-0.464 Picture PostScript Text
3' UTR -1019.703326-0.307 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012340 - NA-bd_OB-fold
IPR016027 - NA-bd_OB-fold-like
IPR019769 - Trans_elong_IF5A_hypusine_site
IPR001884 - Transl_elong_IF5A
IPR020189 - Transl_elong_IF5A_C
IPR014722 - Transl_SH3-like_sub
IPR008991 - Translation_prot_SH3-like

Pfam Domains:
PF01287 - Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold

SCOP Domains:
50104 - Translation proteins SH3-like domain
50249 - Nucleic acid-binding proteins

ModBase Predicted Comparative 3D Structure on Q6IS14
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0003746 translation elongation factor activity
GO:0043022 ribosome binding

Biological Process:
GO:0006412 translation
GO:0006414 translational elongation
GO:0006452 translational frameshifting
GO:0015031 protein transport
GO:0045901 positive regulation of translational elongation
GO:0045905 positive regulation of translational termination
GO:0051028 mRNA transport

Cellular Component:
GO:0005634 nucleus
GO:0005643 nuclear pore
GO:0005737 cytoplasm
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  JC506666 - Sequence 34 from Patent EP2733220.
JC737778 - Sequence 34 from Patent WO2014075939.
JC506661 - Sequence 29 from Patent EP2733220.
JC737773 - Sequence 29 from Patent WO2014075939.
JC506684 - Sequence 52 from Patent EP2733220.
JC737796 - Sequence 52 from Patent WO2014075939.
JC506688 - Sequence 56 from Patent EP2733220.
JC737800 - Sequence 56 from Patent WO2014075939.
JD211267 - Sequence 192291 from Patent EP1572962.
JC506674 - Sequence 42 from Patent EP2733220.
JC737786 - Sequence 42 from Patent WO2014075939.
JD479054 - Sequence 460078 from Patent EP1572962.
BC070048 - Homo sapiens eukaryotic translation initiation factor 5A-like 1, mRNA (cDNA clone IMAGE:5271164), partial cds.
LP895787 - Sequence 651 from Patent EP3253886.
DQ586707 - Homo sapiens piRNA piR-53819, complete sequence.
DQ583836 - Homo sapiens piRNA piR-50948, complete sequence.
DQ593888 - Homo sapiens piRNA piR-34000, complete sequence.
DQ586986 - Homo sapiens piRNA piR-54098, complete sequence.
JD292292 - Sequence 273316 from Patent EP1572962.
JD464236 - Sequence 445260 from Patent EP1572962.
JD415171 - Sequence 396195 from Patent EP1572962.
JD231883 - Sequence 212907 from Patent EP1572962.
JD512878 - Sequence 493902 from Patent EP1572962.
JD427269 - Sequence 408293 from Patent EP1572962.
JD393811 - Sequence 374835 from Patent EP1572962.
JD390664 - Sequence 371688 from Patent EP1572962.
JD151844 - Sequence 132868 from Patent EP1572962.
JD523284 - Sequence 504308 from Patent EP1572962.
JD449977 - Sequence 431001 from Patent EP1572962.
JD445355 - Sequence 426379 from Patent EP1572962.
JD178159 - Sequence 159183 from Patent EP1572962.
JD285876 - Sequence 266900 from Patent EP1572962.
JD239904 - Sequence 220928 from Patent EP1572962.
JD502189 - Sequence 483213 from Patent EP1572962.
DQ583326 - Homo sapiens piRNA piR-50438, complete sequence.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000520547.1, ENST00000520547.2, ENST00000520547.3, IF5AL_HUMAN, NM_001099692, Q6IS14, uc323tuv.1, uc323tuv.2
UCSC ID: ENST00000520547.4_4
RefSeq Accession: NM_001099692.2
Protein: Q6IS14 (aka IF5AL_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.