Human Gene EIF5B (ENST00000289371.11_7) from GENCODE V47lift37
  Description: eukaryotic translation initiation factor 5B (from RefSeq NM_015904.4)
Gencode Transcript: ENST00000289371.11_7
Gencode Gene: ENSG00000158417.12_12
Transcript (Including UTRs)
   Position: hg19 chr2:99,953,852-100,017,789 Size: 63,938 Total Exon Count: 24 Strand: +
Coding Region
   Position: hg19 chr2:99,954,018-100,015,877 Size: 61,860 Coding Exon Count: 24 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:99,953,852-100,017,789)mRNA (may differ from genome)Protein (1220 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: IF2P_HUMAN
DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 5B; Short=eIF-5B; AltName: Full=Translation initiation factor IF-2;
FUNCTION: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).
SUBUNIT: Interacts with ANXA5 in a calcium and phospholipid- dependent manner (By similarity).
INTERACTION: O14602:EIF1AY; NbExp=2; IntAct=EBI-928530, EBI-286439; P14921:ETS1; NbExp=2; IntAct=EBI-928530, EBI-913209;
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the IF-2 family.
SEQUENCE CAUTION: Sequence=BAA34461.2; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: EIF5B
Diseases sorted by gene-association score: inflammatory liposarcoma (5), adult medulloblastoma (1), skeletal tuberculosis (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 65.41 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 1795.85 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -55.30166-0.333 Picture PostScript Text
3' UTR -419.801912-0.220 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000795 - ProtSyn_GTP-bd
IPR005225 - Small_GTP-bd_dom
IPR015760 - TIF_IF2
IPR023115 - TIF_IF2_dom3
IPR004161 - Transl_elong_EFTu/EF1A_2
IPR009000 - Transl_elong_init/rib_B-barrel

Pfam Domains:
PF00009 - Elongation factor Tu GTP binding domain
PF01926 - 50S ribosome-binding GTPase
PF03144 - Elongation factor Tu domain 2
PF11987 - Translation-initiation factor 2

SCOP Domains:
50447 - Translation proteins
52156 - Initiation factor IF2/eIF5b, domain 3
52540 - P-loop containing nucleoside triphosphate hydrolases

ModBase Predicted Comparative 3D Structure on O60841
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003723 RNA binding
GO:0003743 translation initiation factor activity
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0016787 hydrolase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006412 translation
GO:0006413 translational initiation
GO:0006446 regulation of translational initiation

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  AK308176 - Homo sapiens cDNA, FLJ98124.
AB018284 - Homo sapiens KIAA0741 mRNA for KIAA0741 protein.
AJ006776 - Homo sapiens mRNA for translation initiation factor 2 (IF2 gene).
AF078035 - Homo sapiens translation initiation factor IF2 mRNA, complete cds.
BC032639 - Homo sapiens eukaryotic translation initiation factor 5B, mRNA (cDNA clone MGC:44860 IMAGE:5496504), complete cds.
AB383972 - Synthetic construct DNA, clone: pF1KSDA0741, Homo sapiens EIF5B gene for eukaryotic translation initiation factor 5B, complete cds, without stop codon, in Flexi system.
KJ898069 - Synthetic construct Homo sapiens clone ccsbBroadEn_07463 EIF5B gene, encodes complete protein.
AL133563 - Homo sapiens mRNA; cDNA DKFZp434I036 (from clone DKFZp434I036); partial cds.
AK001386 - Homo sapiens cDNA FLJ10524 fis, clone NT2RP2000880, highly similar to Eukaryotic translation initiation factor 5B.
AJ006412 - Homo sapiens mRNA for putative GTP-binding protein, partial.
AK308175 - Homo sapiens cDNA, FLJ98123.
JD370058 - Sequence 351082 from Patent EP1572962.
BC022260 - Homo sapiens eukaryotic translation initiation factor 5B, mRNA (cDNA clone IMAGE:4804924), partial cds.
BC006970 - Homo sapiens eukaryotic translation initiation factor 5B, mRNA (cDNA clone IMAGE:4041983), partial cds.
CU689458 - Synthetic construct Homo sapiens gateway clone IMAGE:100020589 5' read EIF5B mRNA.
AK091864 - Homo sapiens cDNA FLJ34545 fis, clone HLUNG2008958.
JD177092 - Sequence 158116 from Patent EP1572962.
JD297503 - Sequence 278527 from Patent EP1572962.
JD235855 - Sequence 216879 from Patent EP1572962.
JD230711 - Sequence 211735 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O60841 (Reactome details) participates in the following event(s):

R-HSA-72671 eIF5B:GTP is hydrolyzed and released
R-HSA-72672 The 60S subunit joins the translation initiation complex
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72737 Cap-dependent Translation Initiation
R-HSA-72613 Eukaryotic Translation Initiation
R-HSA-72766 Translation
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000289371.1, ENST00000289371.10, ENST00000289371.2, ENST00000289371.3, ENST00000289371.4, ENST00000289371.5, ENST00000289371.6, ENST00000289371.7, ENST00000289371.8, ENST00000289371.9, IF2, IF2P_HUMAN, KIAA0741, NM_015904, O60841, O95805, Q53RV7, Q53SI8, Q9UF81, Q9UMN7, uc317kru.1, uc317kru.2
UCSC ID: ENST00000289371.11_7
RefSeq Accession: NM_015904.4
Protein: O60841 (aka IF2P_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.