Human Gene ENO2 (ENST00000229277.6_5) from GENCODE V47lift37
  Description: enolase 2 (from RefSeq NM_001975.3)
Gencode Transcript: ENST00000229277.6_5
Gencode Gene: ENSG00000111674.9_10
Transcript (Including UTRs)
   Position: hg19 chr12:7,023,744-7,032,860 Size: 9,117 Total Exon Count: 12 Strand: +
Coding Region
   Position: hg19 chr12:7,024,997-7,031,963 Size: 6,967 Coding Exon Count: 11 

Page IndexSequence and LinksPrimersMalaCardsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:7,023,744-7,032,860)mRNA (may differ from genome)Protein (434 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: ENO2
Diseases sorted by gene-association score: granular cell tumor (36), neuroendocrine tumor (26), melanotic neuroectodermal tumor (25), askin's tumor (25), cranial nerve malignant neoplasm (24), middle ear adenoma (24), ossifying fibromyxoid tumor (24), creutzfeldt-jakob disease (23), pediatric ovarian dysgerminoma (23), olfactory neuroblastoma (23), small cell osteogenic sarcoma (22), cerebellar medulloblastoma (22), miliary tuberculosis (22), adrenal neuroblastoma (21), hemangioblastoma (21), small cell carcinoma (19), pandas (18), sinonasal undifferentiated carcinoma (18), malignant ectomesenchymoma (18), merkel cell carcinoma (18), intracranial cysts (17), retroperitoneal neuroblastoma (17), small cell cancer of the lung, somatic (17), neonatal hypoxic and ischemic brain injury (17), olfactory nerve neoplasm (17), integumentary system benign neoplasm (17), microcystic adenoma (16), astroblastoma (16), status epilepticus (16), sensory organ benign neoplasm (16), lymphoepithelioma-like carcinoma (16), cervix small cell carcinoma (16), pineal parenchymal tumor of intermediate differentiation (16), cerebral neuroblastoma (16), middle cerebral artery infarction (15), endometrial small cell carcinoma (15), large cell carcinoma (15), ganglioglioma (15), rete testis adenocarcinoma (15), small cell neuroendocrine carcinoma (14), carcinoid tumors, intestinal (14), lung meningioma (14), cardiac arrest (14), gastric gastrinoma (14), lung cancer (13), ewing sarcoma (13), primitive neuroectodermal tumor of the cervix uteri (13), alveolar soft-part sarcoma (13), spondylosis (13), teratoma (13), ectomesenchymoma (12), brain cancer (12), optic nerve glioma (12), supraglottis cancer (12), neuroma (12), large cell neuroendocrine carcinoma (12), basaloid squamous cell carcinoma (12), central neurocytoma (11), paraganglioma (11), cystic teratoma (11), malignant granular cell myoblastoma (11), traumatic brain injury (11), respiratory system benign neoplasm (11), auditory system cancer (11), malignant sertoli cell tumor (11), pediatric ovarian germ cell tumor (11), cutaneous ganglioneuroma (11), head injury (10), large cell carcinoma with rhabdoid phenotype (10), small cell carcinoma of the bladder (10), epithelioid leiomyosarcoma (10), bednar tumor (10), lymphocytic hypophysitis (10), gastrointestinal stromal tumor (10), clear cell ependymoma (10), autoimmune retinopathy (10), extrahepatic bile duct adenocarcinoma (10), seminoma (10), cerebral ventricle cancer (10), supratentorial cancer (10), cerebrum cancer (10), multiple mucosal neuroma (9), glomus tumor (9), lambert-eaton myasthenic syndrome (9), prion disease (9), subependymal giant cell astrocytoma (9), bone benign neoplasm (9), ganglioneuroma (9), akinetic mutism (9), myoblastoma (9), bone ewing's sarcoma (9), duodenum cancer (9), cauda equina neoplasm (9), lung benign neoplasm (8), urinary bladder small cell neuroendocrine carcinoma (8), rete testis neoplasm (8), intracranial primitive neuroectodermal tumor (8), ganglioneuroblastoma (8), angiomyoma (8), papillary tumor of the pineal region (8), leiomyosarcoma (8), choriocarcinoma of ovary (8), epulis (8), congenital epulis (8), central nervous system primitive neuroectodermal neoplasm (8), goblet cell carcinoid (8), sclerosing hemangioma (8), subependymoma (8), bronchus cancer (8), gastric small cell carcinoma (8), neurilemmoma (8), brain injury (8), central nervous system melanocytic neoplasm (8), congenital granular cell tumor (8), desmoplastic small round cell tumor (8), cerebellar liponeurocytoma (8), transitional cell carcinoma (8), extraskeletal ewing sarcoma (7), small intestine cancer (7), extraskeletal mesenchymal chondrosarcoma (7), adrenal carcinoma (7), granulosa cell tumor of the ovary (7), secondary progressive multiple sclerosis (7), peripheral nervous system neoplasm (7), duodenal somatostatinoma (7), prostate small cell carcinoma (7), autonomic nervous system neoplasm (7), persistent vegetative state (7), pineal gland cancer (7), clear cell sarcoma (7), cancer-associated retinopathy (7), extraosseous chondrosarcoma (7), mature teratoma of the ovary (7), melanotic medulloblastoma (7), appendicitis (7), gallbladder small cell carcinoma (7), atypical follicular adenoma (7), malignant teratoma (7), conventional fibrosarcoma (7), nervous system benign neoplasm (7), epithelioid malignant peripheral nerve sheath tumor (7), plexiform schwannoma (7), rhabdomyosarcoma 2, alveolar (7), papillary ependymoma (7), dysentery (7), spindle cell carcinoma (7), achalasia (7), connective tissue benign neoplasm (7), scrapie (6), orbital cancer (6), chondrosarcoma (6), meningothelial meningioma (6), mongolian spot (6), meningeal melanocytoma (6), sex cord-gonadal stromal tumor (6), finger agnosia (6), gastric leiomyosarcoma (6), benign ependymoma (6), paraneoplastic cerebellar degeneration (6), chondrosarcoma, extraskeletal myxoid (6), pancreatic somatostatinoma (6), dysgerminoma of ovary (6), horseshoe kidney (6), pleurisy (6), subependymal glioma (6), oligodendroglioma (6), cerebellum cancer (6), pancoast tumor (6), pineocytoma (6), chondroid lipoma (6), nodular prostate (6), epithelioid cell melanoma (6), kidney rhabdoid cancer (5), papillary adenoma (5), skin benign neoplasm (5), mixed ductal-endocrine carcinoma (5), tuberculous meningitis (5), extraosseous ewing's sarcoma (5), leukocoria (5), meningeal melanomatosis (5), acute thyroiditis (5), mixed cell type cancer (5), cell type cancer (5), mucinous ovarian cystadenoma (5), yunis-varon syndrome (5), breast adenomyoepithelioma (5), breast myoepithelial neoplasm (5), carcinoid syndrome (5), nodular ganglioneuroblastoma (5), microcystic meningioma (5), organ system benign neoplasm (5), heart sarcoma (5), pleomorphic adenoma carcinoma (5), chondroid chordoma (5), cervical clear cell adenocarcinoma (5), nervous system cancer (4), cerebral primitive neuroectodermal tumor (4), intraocular retinoblastoma (4), gastric squamous cell carcinoma (4), acute mountain sickness (4), botryoid rhabdomyosarcoma (4), ovarian mucinous neoplasm (4), cloacogenic carcinoma (4), medullomyoblastoma (4), rhabdoid cancer (4), liver angiosarcoma (4), pericardial mesothelioma (4), glomangioma (4), spinal canal and spinal cord meningioma (4), cardiovascular organ benign neoplasm (4), benign perivascular tumor (4), diffuse pulmonary fibrosis (4), pericardium cancer (4), rubeosis iridis (4), spinal meningioma (4), optic nerve neoplasm (4), adenosquamous cell lung carcinoma (4), ideomotor apraxia (3), juvenile pilocytic astrocytoma (3), rhabdomyosarcoma (3), gliosarcoma (3), retinal hemangioblastoma (3), adult medulloblastoma (3), medulloblastoma (3), pheochromocytoma (2), prostate cancer (2), medullary thyroid carcinoma, familial (2), hydrocephalus (2), stroke, ischemic (2), wilms tumor susceptibility-5 (2), neuroblastoma (1), renal cell carcinoma (1), muscle cancer (1), connective tissue cancer (1), integumentary system cancer (1), endocrine gland cancer (1), leber congenital amaurosis (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 516.84 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 3317.50 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -12.6092-0.137 Picture PostScript Text
3' UTR -278.90897-0.311 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000941 - Enolase
IPR020810 - Enolase_C
IPR020809 - Enolase_CS
IPR020811 - Enolase_N

Pfam Domains:
PF00113 - Enolase, C-terminal TIM barrel domain
PF03952 - Enolase, N-terminal domain
PF07476 - Methylaspartate ammonia-lyase C-terminus
PF13378 - Enolase C-terminal domain-like

SCOP Domains:
51604 - Enolase C-terminal domain-like
54826 - Enolase N-terminal domain-like

ModBase Predicted Comparative 3D Structure on Q6FHV6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000287 magnesium ion binding
GO:0004634 phosphopyruvate hydratase activity
GO:0005515 protein binding
GO:0046872 metal ion binding

Biological Process:
GO:0006096 glycolytic process

Cellular Component:
GO:0000015 phosphopyruvate hydratase complex
GO:0001917 photoreceptor inner segment
GO:0005622 intracellular
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0043025 neuronal cell body
GO:0043204 perikaryon
GO:0043209 myelin sheath


-  Descriptions from all associated GenBank mRNAs
  AK290525 - Homo sapiens cDNA FLJ77877 complete cds, highly similar to Human ENO2 gene for neuron specific (gamma) enolase.
LF207388 - JP 2014500723-A/14891: Polycomb-Associated Non-Coding RNAs.
LF213061 - JP 2014500723-A/20564: Polycomb-Associated Non-Coding RNAs.
AK312402 - Homo sapiens cDNA, FLJ92734, Homo sapiens enolase 2, (gamma, neuronal) (ENO2), mRNA.
AK295220 - Homo sapiens cDNA FLJ50150 complete cds, highly similar to Gamma-enolase (EC 4.2.1.11).
BC002745 - Homo sapiens enolase 2 (gamma, neuronal), mRNA (cDNA clone MGC:3520 IMAGE:3629603), complete cds.
M22349 - Human neuron-specific gamma-2 enolase, complete cds.
JD458605 - Sequence 439629 from Patent EP1572962.
JD056188 - Sequence 37212 from Patent EP1572962.
JD189472 - Sequence 170496 from Patent EP1572962.
JD493820 - Sequence 474844 from Patent EP1572962.
AK124656 - Homo sapiens cDNA FLJ42665 fis, clone BRAMY2019989, highly similar to Gamma-enolase (EC 4.2.1.11).
DQ893819 - Synthetic construct Homo sapiens clone IMAGE:100008279; FLH164695.01L; RZPDo839A11157D enolase 2 (gamma, neuronal) (ENO2) gene, encodes complete protein.
EU176168 - Synthetic construct Homo sapiens clone IMAGE:100006336; FLH164702.01X; RZPDo839C01250D enolase 2 (gamma, neuronal) (ENO2) gene, encodes complete protein.
CU678160 - Synthetic construct Homo sapiens gateway clone IMAGE:100019381 5' read ENO2 mRNA.
AB529145 - Synthetic construct DNA, clone: pF1KB5183, Homo sapiens ENO2 gene for enolase 2, without stop codon, in Flexi system.
KJ896767 - Synthetic construct Homo sapiens clone ccsbBroadEn_06161 ENO2 gene, encodes complete protein.
BT007383 - Homo sapiens enolase 2, (gamma, neuronal) mRNA, complete cds.
EU794607 - Homo sapiens epididymis secretory protein Li 279 (HEL-S-279) mRNA, complete cds.
CR536582 - Homo sapiens full open reading frame cDNA clone RZPDo834F1122D for gene ENO2, enolase 2, (gamma, neuronal); complete cds, incl. stopcodon.
X13120 - Human mRNA fragment for neurone-specific enolase.
X14327 - Human mRNA for neurone specific gamma enolase (NSE), EC number=4.2.1.11.
M36768 - Human gamma enolase mRNA, 3' end.
LF365836 - JP 2014500723-A/173339: Polycomb-Associated Non-Coding RNAs.
Y00691 - H.spaiens 3' mRNA for neurone-specific enolase (EC 4.2.1.11).
JD273660 - Sequence 254684 from Patent EP1572962.
JD204801 - Sequence 185825 from Patent EP1572962.
JD496377 - Sequence 477401 from Patent EP1572962.
JD468164 - Sequence 449188 from Patent EP1572962.
JD264804 - Sequence 245828 from Patent EP1572962.
JD521767 - Sequence 502791 from Patent EP1572962.
JD467730 - Sequence 448754 from Patent EP1572962.
JD559531 - Sequence 540555 from Patent EP1572962.
JD297413 - Sequence 278437 from Patent EP1572962.
JD101527 - Sequence 82551 from Patent EP1572962.
JD090630 - Sequence 71654 from Patent EP1572962.
JD026874 - Sequence 7898 from Patent EP1572962.
JD028013 - Sequence 9037 from Patent EP1572962.
JD484197 - Sequence 465221 from Patent EP1572962.
LF365835 - JP 2014500723-A/173338: Polycomb-Associated Non-Coding RNAs.
JD085781 - Sequence 66805 from Patent EP1572962.
JD427794 - Sequence 408818 from Patent EP1572962.
JD501403 - Sequence 482427 from Patent EP1572962.
JD297861 - Sequence 278885 from Patent EP1572962.
JD417955 - Sequence 398979 from Patent EP1572962.
JD261317 - Sequence 242341 from Patent EP1572962.
JD371781 - Sequence 352805 from Patent EP1572962.
JD124191 - Sequence 105215 from Patent EP1572962.
JD288853 - Sequence 269877 from Patent EP1572962.
JD051902 - Sequence 32926 from Patent EP1572962.
JD527941 - Sequence 508965 from Patent EP1572962.
MA601413 - JP 2018138019-A/173339: Polycomb-Associated Non-Coding RNAs.
MA601412 - JP 2018138019-A/173338: Polycomb-Associated Non-Coding RNAs.
MA442965 - JP 2018138019-A/14891: Polycomb-Associated Non-Coding RNAs.
MA448638 - JP 2018138019-A/20564: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY66-399 - gluconeogenesis
PWY66-400 - glycolysis
PWY66-407 - superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle

-  Other Names for This Gene
  Alternate Gene Symbols: ENO2 , ENST00000229277.1, ENST00000229277.2, ENST00000229277.3, ENST00000229277.4, ENST00000229277.5, hCG_25937 , HEL-S-279 , NM_001975, Q6FHV6, Q6FHV6_HUMAN, uc317dqd.1, uc317dqd.2
UCSC ID: ENST00000229277.6_5
RefSeq Accession: NM_001975.3
Protein: Q6FHV6

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.