Human Gene F2RL3 (ENST00000248076.4_4) from GENCODE V47lift37
  Description: F2R like thrombin or trypsin receptor 3 (from RefSeq NM_003950.4)
Gencode Transcript: ENST00000248076.4_4
Gencode Gene: ENSG00000127533.4_7
Transcript (Including UTRs)
   Position: hg19 chr19:16,999,810-17,003,417 Size: 3,608 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr19:17,000,001-17,001,432 Size: 1,432 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:16,999,810-17,003,417)mRNA (may differ from genome)Protein (385 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PAR4_HUMAN
DESCRIPTION: RecName: Full=Proteinase-activated receptor 4; Short=PAR-4; AltName: Full=Coagulation factor II receptor-like 3; AltName: Full=Thrombin receptor-like 3; Flags: Precursor;
FUNCTION: Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.
PTM: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/f2rl3/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 19.37 RPKM in Lung
Total median expression: 85.27 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -84.90191-0.445 Picture PostScript Text
3' UTR -785.001985-0.395 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR003944 - Prot_act_rcpt_4
IPR003912 - Protea_act_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)
PF10320 - Serpentine type 7TM GPCR chemoreceptor Srsx

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2ZPK - X-ray 3QDZ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q96RI0
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0015057 thrombin-activated receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007200 phospholipase C-activating G-protein coupled receptor signaling pathway
GO:0007596 blood coagulation
GO:0007599 hemostasis
GO:0009611 response to wounding
GO:0030168 platelet activation
GO:0035025 positive regulation of Rho protein signal transduction
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
GO:0051482 positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
GO:0060155 platelet dense granule organization
GO:0070493 thrombin-activated receptor signaling pathway

Cellular Component:
GO:0005576 extracellular region
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AF055917 - Homo sapiens protease-activated receptor 4 mRNA, complete cds.
JD450220 - Sequence 431244 from Patent EP1572962.
JD271838 - Sequence 252862 from Patent EP1572962.
JD399341 - Sequence 380365 from Patent EP1572962.
JD372527 - Sequence 353551 from Patent EP1572962.
JD317448 - Sequence 298472 from Patent EP1572962.
BC074782 - Homo sapiens coagulation factor II (thrombin) receptor-like 3, mRNA (cDNA clone MGC:103944 IMAGE:30915337), complete cds.
AF080214 - Homo sapiens protease-activated receptor 4 mRNA, complete cds.
AY431102 - Homo sapiens coagulation factor II/thrombin receptor-like 3 protein (F2RL3) mRNA, complete cds.
JD450673 - Sequence 431697 from Patent EP1572962.
JD520014 - Sequence 501038 from Patent EP1572962.
JD462324 - Sequence 443348 from Patent EP1572962.
JD521356 - Sequence 502380 from Patent EP1572962.
JD179346 - Sequence 160370 from Patent EP1572962.
JD381003 - Sequence 362027 from Patent EP1572962.
JD362019 - Sequence 343043 from Patent EP1572962.
JD430967 - Sequence 411991 from Patent EP1572962.
JD558793 - Sequence 539817 from Patent EP1572962.
JD532282 - Sequence 513306 from Patent EP1572962.
JD373405 - Sequence 354429 from Patent EP1572962.
JD340650 - Sequence 321674 from Patent EP1572962.
JD286458 - Sequence 267482 from Patent EP1572962.
JD246092 - Sequence 227116 from Patent EP1572962.
JD379136 - Sequence 360160 from Patent EP1572962.
JD410100 - Sequence 391124 from Patent EP1572962.
JD083263 - Sequence 64287 from Patent EP1572962.
JD359941 - Sequence 340965 from Patent EP1572962.
JD426047 - Sequence 407071 from Patent EP1572962.
JD165709 - Sequence 146733 from Patent EP1572962.
JD204830 - Sequence 185854 from Patent EP1572962.
JD421416 - Sequence 402440 from Patent EP1572962.
JD421417 - Sequence 402441 from Patent EP1572962.
JD178940 - Sequence 159964 from Patent EP1572962.
JD178941 - Sequence 159965 from Patent EP1572962.
JD176487 - Sequence 157511 from Patent EP1572962.
JD066688 - Sequence 47712 from Patent EP1572962.
JD183983 - Sequence 165007 from Patent EP1572962.
JD558984 - Sequence 540008 from Patent EP1572962.
JD537911 - Sequence 518935 from Patent EP1572962.
JD461362 - Sequence 442386 from Patent EP1572962.
JD434048 - Sequence 415072 from Patent EP1572962.
JD198013 - Sequence 179037 from Patent EP1572962.
JD481193 - Sequence 462217 from Patent EP1572962.
JD471780 - Sequence 452804 from Patent EP1572962.
JD430616 - Sequence 411640 from Patent EP1572962.
JD557663 - Sequence 538687 from Patent EP1572962.
JD436834 - Sequence 417858 from Patent EP1572962.
JD341080 - Sequence 322104 from Patent EP1572962.
JD565649 - Sequence 546673 from Patent EP1572962.
JD215031 - Sequence 196055 from Patent EP1572962.
JD117103 - Sequence 98127 from Patent EP1572962.
JD277614 - Sequence 258638 from Patent EP1572962.
JD482661 - Sequence 463685 from Patent EP1572962.
JD497138 - Sequence 478162 from Patent EP1572962.
JD275492 - Sequence 256516 from Patent EP1572962.
JD324122 - Sequence 305146 from Patent EP1572962.
JD084429 - Sequence 65453 from Patent EP1572962.
JD247826 - Sequence 228850 from Patent EP1572962.
JD101565 - Sequence 82589 from Patent EP1572962.
JD326954 - Sequence 307978 from Patent EP1572962.
JD431130 - Sequence 412154 from Patent EP1572962.
JD291434 - Sequence 272458 from Patent EP1572962.
JD532497 - Sequence 513521 from Patent EP1572962.
JD443771 - Sequence 424795 from Patent EP1572962.
JD071156 - Sequence 52180 from Patent EP1572962.
JD194391 - Sequence 175415 from Patent EP1572962.
JD194392 - Sequence 175416 from Patent EP1572962.
JD401853 - Sequence 382877 from Patent EP1572962.
JD232055 - Sequence 213079 from Patent EP1572962.
JD444137 - Sequence 425161 from Patent EP1572962.
JD337376 - Sequence 318400 from Patent EP1572962.
JD173322 - Sequence 154346 from Patent EP1572962.
JD167483 - Sequence 148507 from Patent EP1572962.
JD559908 - Sequence 540932 from Patent EP1572962.
JD474499 - Sequence 455523 from Patent EP1572962.
JD186536 - Sequence 167560 from Patent EP1572962.
JD159691 - Sequence 140715 from Patent EP1572962.
JD211901 - Sequence 192925 from Patent EP1572962.
JD196763 - Sequence 177787 from Patent EP1572962.
JD410489 - Sequence 391513 from Patent EP1572962.
JD112160 - Sequence 93184 from Patent EP1572962.
JD211750 - Sequence 192774 from Patent EP1572962.
JD145296 - Sequence 126320 from Patent EP1572962.
JD241311 - Sequence 222335 from Patent EP1572962.
JD278849 - Sequence 259873 from Patent EP1572962.
JD472650 - Sequence 453674 from Patent EP1572962.
JD323009 - Sequence 304033 from Patent EP1572962.
JD401723 - Sequence 382747 from Patent EP1572962.
JD269596 - Sequence 250620 from Patent EP1572962.
JD366458 - Sequence 347482 from Patent EP1572962.
JD149393 - Sequence 130417 from Patent EP1572962.
JD330597 - Sequence 311621 from Patent EP1572962.
JD062277 - Sequence 43301 from Patent EP1572962.
JD497529 - Sequence 478553 from Patent EP1572962.
JD542119 - Sequence 523143 from Patent EP1572962.
JD462425 - Sequence 443449 from Patent EP1572962.
JD098235 - Sequence 79259 from Patent EP1572962.
JD380026 - Sequence 361050 from Patent EP1572962.
JD334739 - Sequence 315763 from Patent EP1572962.
JD064028 - Sequence 45052 from Patent EP1572962.
JD438252 - Sequence 419276 from Patent EP1572962.
JD277004 - Sequence 258028 from Patent EP1572962.
JD204255 - Sequence 185279 from Patent EP1572962.
JD541615 - Sequence 522639 from Patent EP1572962.
JD435720 - Sequence 416744 from Patent EP1572962.
JD041848 - Sequence 22872 from Patent EP1572962.
JD284708 - Sequence 265732 from Patent EP1572962.
JD456684 - Sequence 437708 from Patent EP1572962.
JD157883 - Sequence 138907 from Patent EP1572962.
JD204811 - Sequence 185835 from Patent EP1572962.
JD330047 - Sequence 311071 from Patent EP1572962.
JD065137 - Sequence 46161 from Patent EP1572962.
JD234617 - Sequence 215641 from Patent EP1572962.
JD391839 - Sequence 372863 from Patent EP1572962.
JD150563 - Sequence 131587 from Patent EP1572962.
JD134782 - Sequence 115806 from Patent EP1572962.
JD535824 - Sequence 516848 from Patent EP1572962.
JD199945 - Sequence 180969 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_sppaPathway - Aspirin Blocks Signaling Pathway Involved in Platelet Activation
h_Par1Pathway - Thrombin signaling and protease-activated receptors

Reactome (by CSHL, EBI, and GO)

Protein Q96RI0 (Reactome details) participates in the following event(s):

R-HSA-389463 Proteinase-activated receptors can bind thrombin
R-HSA-114697 Thrombin-mediated activation of Proteinase-activated receptors
R-HSA-396941 Thrombin-activated PAR binds G-protein G12/13
R-HSA-396996 Thrombin-activated PAR binds G-protein Gq
R-HSA-397835 Dissociation of the PAR:Gq complex
R-HSA-397891 Dissociation of the PAR:G12/13 complex
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-114558 Gq activation by PAR
R-HSA-114552 G12/13 activation by PAR
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-456926 Thrombin signalling through proteinase activated receptors (PARs)
R-HSA-416476 G alpha (q) signalling events
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-76002 Platelet activation, signaling and aggregation
R-HSA-388396 GPCR downstream signalling
R-HSA-500792 GPCR ligand binding
R-HSA-109582 Hemostasis
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000248076.1, ENST00000248076.2, ENST00000248076.3, NM_003950, O76067, PAR4, PAR4_HUMAN, Q6DK42, Q96RI0, uc317ews.1, uc317ews.2
UCSC ID: ENST00000248076.4_4
RefSeq Accession: NM_003950.4
Protein: Q96RI0 (aka PAR4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.