Human Gene FBXL3 (ENST00000355619.10_7) from GENCODE V47lift37
  Description: F-box and leucine rich repeat protein 3 (from RefSeq NM_012158.4)
Gencode Transcript: ENST00000355619.10_7
Gencode Gene: ENSG00000005812.11_17
Transcript (Including UTRs)
   Position: hg19 chr13:77,579,395-77,601,294 Size: 21,900 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr13:77,581,280-77,595,995 Size: 14,716 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr13:77,579,395-77,601,294)mRNA (may differ from genome)Protein (428 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: FBXL3_HUMAN
DESCRIPTION: RecName: Full=F-box/LRR-repeat protein 3; AltName: Full=F-box and leucine-rich repeat protein 3A; AltName: Full=F-box/LRR-repeat protein 3A;
FUNCTION: Substrate-recognition component of some SCF (SKP1-CUL1- F-box protein)-type E3 ubiquitin ligase complex involved in circadian clock function. The SCF(FBXL3) complex acts by mediating ubiquitination and subsequent degradation of CRY1 and CRY2. Recruiter of target protein that may recognize and bind to some phosphorylated proteins and promotes their ubiquitination and degradation.
SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with CRY1 and CRY2.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Predominantly nuclear.
TISSUE SPECIFICITY: Widely expressed.
SIMILARITY: Contains 1 F-box domain.
SIMILARITY: Contains 7 LRR (leucine-rich) repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 27.79 RPKM in Artery - Tibial
Total median expression: 618.02 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -170.20334-0.510 Picture PostScript Text
3' UTR -407.201885-0.216 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001810 - F-box_dom_cyclin-like

Pfam Domains:
PF00646 - F-box domain
PF12937 - F-box-like

SCOP Domains:
81383 - F-box domain
52047 - RNI-like
52058 - L domain-like

ModBase Predicted Comparative 3D Structure on Q9UKT7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding

Biological Process:
GO:0000209 protein polyubiquitination
GO:0016567 protein ubiquitination
GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0031648 protein destabilization
GO:0042752 regulation of circadian rhythm
GO:0043153 entrainment of circadian clock by photoperiod
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0043687 post-translational protein modification
GO:0048511 rhythmic process

Cellular Component:
GO:0000151 ubiquitin ligase complex
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016604 nuclear body
GO:0019005 SCF ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  BC072448 - Homo sapiens F-box and leucine-rich repeat protein 3, mRNA (cDNA clone MGC:90244 IMAGE:6166244), complete cds.
AF126028 - Homo sapiens unknown mRNA.
AL833187 - Homo sapiens mRNA; cDNA DKFZp667K025 (from clone DKFZp667K025).
BC063796 - Homo sapiens cDNA clone IMAGE:6174493, containing frame-shift errors.
AK314442 - Homo sapiens cDNA, FLJ95240, highly similar to Homo sapiens F-box and leucine-rich repeat protein 3 (FBXL3), mRNA.
HQ447499 - Synthetic construct Homo sapiens clone IMAGE:100070835; CCSB014644_02 F-box and leucine-rich repeat protein 3 (FBXL3) gene, encodes complete protein.
KJ893538 - Synthetic construct Homo sapiens clone ccsbBroadEn_02932 FBXL3 gene, encodes complete protein.
AB590818 - Synthetic construct DNA, clone: pFN21AE1144, Homo sapiens FBXL3 gene for F-box and leucine-rich repeat protein 3, without stop codon, in Flexi system.
AF129532 - Homo sapiens chromosome 13 F-box protein Fbl3a (FBL3A) mRNA, partial cds.
CU687236 - Synthetic construct Homo sapiens gateway clone IMAGE:100022901 5' read FBXL3 mRNA.
AK025484 - Homo sapiens cDNA: FLJ21831 fis, clone HEP01486, highly similar to AF126028 Homo sapiens unknown mRNA.
JD299614 - Sequence 280638 from Patent EP1572962.
JD286084 - Sequence 267108 from Patent EP1572962.
JD244448 - Sequence 225472 from Patent EP1572962.
JD355668 - Sequence 336692 from Patent EP1572962.
JD433970 - Sequence 414994 from Patent EP1572962.
JD113701 - Sequence 94725 from Patent EP1572962.
JD502028 - Sequence 483052 from Patent EP1572962.
JD370633 - Sequence 351657 from Patent EP1572962.
JD408062 - Sequence 389086 from Patent EP1572962.
JD195922 - Sequence 176946 from Patent EP1572962.
JD336613 - Sequence 317637 from Patent EP1572962.
JD440553 - Sequence 421577 from Patent EP1572962.
JD461653 - Sequence 442677 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9UKT7 (Reactome details) participates in the following event(s):

R-HSA-400272 FBXL3 binds phosphorylated CRY proteins
R-HSA-390470 Association of CCT/TriC with other substrates during biosynthesis (unknown chaperone)
R-HSA-8952620 NEDD8:AcM-UBE2M binds CRL1 E3 ubiquitin ligase complex
R-HSA-8956200 MyrG-DCUN1D3 binds CRL1 E3 ubiquitin ligase complex
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-983147 Release of E3 from polyubiquitinated substrate
R-HSA-8955241 CAND1 binds cytosolic CRL E3 ubiquitin ligases
R-HSA-8952618 AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex
R-HSA-8955289 COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-8956040 COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-400253 Circadian Clock
R-HSA-390471 Association of TriC/CCT with target proteins during biosynthesis
R-HSA-390466 Chaperonin-mediated protein folding
R-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-391251 Protein folding
R-HSA-597592 Post-translational protein modification
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-392499 Metabolism of proteins
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: B2RB04, ENST00000355619.1, ENST00000355619.2, ENST00000355619.3, ENST00000355619.4, ENST00000355619.5, ENST00000355619.6, ENST00000355619.7, ENST00000355619.8, ENST00000355619.9, FBL3A, FBXL3A, FBXL3_HUMAN, NM_012158, Q9P122, Q9UKT7, uc317zdx.1, uc317zdx.2
UCSC ID: ENST00000355619.10_7
RefSeq Accession: NM_012158.4
Protein: Q9UKT7 (aka FBXL3_HUMAN or FXL3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.