Human Gene FBXO25 (ENST00000350302.8_11) from GENCODE V47lift37
  Description: F-box protein 25, transcript variant 2 (from RefSeq NM_183420.2)
Gencode Transcript: ENST00000350302.8_11
Gencode Gene: ENSG00000147364.17_14
Transcript (Including UTRs)
   Position: hg19 chr8:356,958-427,967 Size: 71,010 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr8:363,080-418,804 Size: 55,725 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:356,958-427,967)mRNA (may differ from genome)Protein (358 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: FBX25_HUMAN
DESCRIPTION: RecName: Full=F-box only protein 25;
FUNCTION: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) (By similarity).
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO25, SKP1, CUL1 and RBX1. Interacts directly with SKP1 and CUL1. Interacts (via C-terminus) with beta-actin (via N- terminus).
INTERACTION: P60709:ACTB; NbExp=3; IntAct=EBI-6264551, EBI-353944;
SUBCELLULAR LOCATION: Nucleus. Note=In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.
TISSUE SPECIFICITY: Expressed in all brain tissue observed.
DOMAIN: The F-box is necessary for the interaction with SKP1 (By similarity).
DISEASE: Note=A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
SIMILARITY: Contains 1 F-box domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 52.49 RPKM in Testis
Total median expression: 334.58 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -59.20116-0.510 Picture PostScript Text
3' UTR -2476.929163-0.270 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001810 - F-box_dom_cyclin-like

SCOP Domains:
81383 - F-box domain

ModBase Predicted Comparative 3D Structure on Q8TCJ0
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003779 actin binding
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding

Biological Process:
GO:0016567 protein ubiquitination

Cellular Component:
GO:0000151 ubiquitin ligase complex
GO:0005634 nucleus
GO:0019005 SCF ubiquitin ligase complex
GO:0005730 nucleolus


-  Descriptions from all associated GenBank mRNAs
  AF419858 - Homo sapiens unknown mRNA.
LF384338 - JP 2014500723-A/191841: Polycomb-Associated Non-Coding RNAs.
BC050393 - Homo sapiens F-box protein 25, mRNA (cDNA clone MGC:51975 IMAGE:5769518), complete cds.
AK302334 - Homo sapiens cDNA FLJ52290 complete cds, highly similar to F-box only protein 25.
BC012143 - Homo sapiens cDNA clone IMAGE:4651986, containing frame-shift errors.
BC020249 - Homo sapiens F-box protein 25, mRNA (cDNA clone MGC:32038 IMAGE:4652811), complete cds.
AB070270 - Homo sapiens mRNA for F-box domain Fbx25-containing protein, complete cds.
CU676526 - Synthetic construct Homo sapiens gateway clone IMAGE:100022444 5' read FBXO25 mRNA.
HQ447709 - Synthetic construct Homo sapiens clone IMAGE:100071037; CCSB013505_01 F-box protein 25 (FBXO25) gene, encodes complete protein.
KJ893548 - Synthetic construct Homo sapiens clone ccsbBroadEn_02942 FBXO25 gene, encodes complete protein.
AF174605 - Homo sapiens F-box protein Fbx25 (FBX25) mRNA, partial cds.
MA619915 - JP 2018138019-A/191841: Polycomb-Associated Non-Coding RNAs.
JD487645 - Sequence 468669 from Patent EP1572962.
LF371332 - JP 2014500723-A/178835: Polycomb-Associated Non-Coding RNAs.
JD204132 - Sequence 185156 from Patent EP1572962.
JD091272 - Sequence 72296 from Patent EP1572962.
MA606909 - JP 2018138019-A/178835: Polycomb-Associated Non-Coding RNAs.
BC035555 - Homo sapiens F-box protein 25, mRNA (cDNA clone IMAGE:5185591), partial cds.
DQ594707 - Homo sapiens piRNA piR-60819, complete sequence.
JD392998 - Sequence 374022 from Patent EP1572962.
JD359362 - Sequence 340386 from Patent EP1572962.
DQ586074 - Homo sapiens piRNA piR-53186, complete sequence.
DQ591684 - Homo sapiens piRNA piR-58796, complete sequence.
DQ583926 - Homo sapiens piRNA piR-51038, complete sequence.
DQ582491 - Homo sapiens piRNA piR-32603, complete sequence.
AK091672 - Homo sapiens cDNA FLJ34353 fis, clone FEBRA2011665.
AX747122 - Sequence 647 from Patent EP1308459.
JD051247 - Sequence 32271 from Patent EP1572962.
JD486720 - Sequence 467744 from Patent EP1572962.
AK056623 - Homo sapiens cDNA FLJ32061 fis, clone OCBBF1000030.
JD263291 - Sequence 244315 from Patent EP1572962.
JD184453 - Sequence 165477 from Patent EP1572962.
JD500201 - Sequence 481225 from Patent EP1572962.
JD082778 - Sequence 63802 from Patent EP1572962.
JD295134 - Sequence 276158 from Patent EP1572962.
JD093635 - Sequence 74659 from Patent EP1572962.
JD236388 - Sequence 217412 from Patent EP1572962.
JD354397 - Sequence 335421 from Patent EP1572962.
JD347290 - Sequence 328314 from Patent EP1572962.
JD486848 - Sequence 467872 from Patent EP1572962.
JD512277 - Sequence 493301 from Patent EP1572962.
JD291950 - Sequence 272974 from Patent EP1572962.
JD352275 - Sequence 333299 from Patent EP1572962.
JD503860 - Sequence 484884 from Patent EP1572962.
JD090201 - Sequence 71225 from Patent EP1572962.
JD279866 - Sequence 260890 from Patent EP1572962.
JD555019 - Sequence 536043 from Patent EP1572962.
JD127217 - Sequence 108241 from Patent EP1572962.
JD504135 - Sequence 485159 from Patent EP1572962.
JD242329 - Sequence 223353 from Patent EP1572962.
JD326074 - Sequence 307098 from Patent EP1572962.
JD309284 - Sequence 290308 from Patent EP1572962.
JD066739 - Sequence 47763 from Patent EP1572962.
JD184066 - Sequence 165090 from Patent EP1572962.
JD098634 - Sequence 79658 from Patent EP1572962.
JD206231 - Sequence 187255 from Patent EP1572962.
JD172404 - Sequence 153428 from Patent EP1572962.
JD093694 - Sequence 74718 from Patent EP1572962.
JD113663 - Sequence 94687 from Patent EP1572962.
JD433438 - Sequence 414462 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000350302.1, ENST00000350302.2, ENST00000350302.3, ENST00000350302.4, ENST00000350302.5, ENST00000350302.6, ENST00000350302.7, FBX25, FBX25_HUMAN, NM_183420, Q6PJ83, Q7Z4V4, Q8TCJ0, Q9UKB8, uc317xyr.1, uc317xyr.2
UCSC ID: ENST00000350302.8_11
RefSeq Accession: NM_183420.2
Protein: Q8TCJ0 (aka FBX25_HUMAN or FX25_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.