Human Gene FBXO43 (ENST00000428847.3_7) from GENCODE V47lift37
  Description: F-box protein 43, transcript variant 1 (from RefSeq NR_036491.2)
Gencode Transcript: ENST00000428847.3_7
Gencode Gene: ENSG00000156509.14_9
Transcript (Including UTRs)
   Position: hg19 chr8:101,145,579-101,158,045 Size: 12,467 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr8:101,146,030-101,157,363 Size: 11,334 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:101,145,579-101,158,045)mRNA (may differ from genome)Protein (708 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: FBX43_HUMAN
DESCRIPTION: RecName: Full=F-box only protein 43; AltName: Full=Endogenous meiotic inhibitor 2;
FUNCTION: Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (Probable).
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with CDC20 (By similarity).
PTM: Phosphorylated on Ser-76, Thr-234 and Ser-334 in response to calcium, which is a prerequisite for ubiquitination and proteasomal degradation (Probable).
PTM: Ubiquitinated in response to calcium, which promotes proteasomal degradation.
SIMILARITY: Contains 1 F-box domain.
SIMILARITY: Contains 1 IBR-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 6.74 RPKM in Testis
Total median expression: 13.31 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -270.40682-0.396 Picture PostScript Text
3' UTR -70.20451-0.156 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002867 - Znf_C6HC

Pfam Domains:
PF00646 - F-box domain

SCOP Domains:
81383 - F-box domain
57850 - RING/U-box

ModBase Predicted Comparative 3D Structure on Q4G163
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0046872 metal ion binding

Biological Process:
GO:0007088 regulation of mitotic nuclear division
GO:0016567 protein ubiquitination
GO:0045835 negative regulation of meiotic nuclear division
GO:0051321 meiotic cell cycle

Cellular Component:
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  BC028709 - Homo sapiens cDNA clone IMAGE:4826921, containing frame-shift errors.
JD063937 - Sequence 44961 from Patent EP1572962.
JD188399 - Sequence 169423 from Patent EP1572962.
JD172468 - Sequence 153492 from Patent EP1572962.
BC090892 - Homo sapiens F-box protein 43, mRNA (cDNA clone IMAGE:30390293).
JD552649 - Sequence 533673 from Patent EP1572962.
JD510590 - Sequence 491614 from Patent EP1572962.
JD173793 - Sequence 154817 from Patent EP1572962.
BC156850 - Synthetic construct Homo sapiens clone IMAGE:100062506, MGC:190533 F-box protein 43 (FBXO43) mRNA, encodes complete protein.
AK310518 - Homo sapiens cDNA, FLJ17560.
JD524364 - Sequence 505388 from Patent EP1572962.
JD200497 - Sequence 181521 from Patent EP1572962.
JD110450 - Sequence 91474 from Patent EP1572962.
JD104697 - Sequence 85721 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: EMI2, ENST00000428847.1, ENST00000428847.2, FBX43_HUMAN, NR_036491, Q4G163, uc319xut.1, uc319xut.2
UCSC ID: ENST00000428847.3_7
RefSeq Accession: NM_001029860.4
Protein: Q4G163 (aka FBX43_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.