Human Gene FBXW8 (ENST00000652555.1_4) from GENCODE V47lift37
  Description: F-box and WD repeat domain containing 8, transcript variant 1 (from RefSeq NM_153348.3)
Gencode Transcript: ENST00000652555.1_4
Gencode Gene: ENSG00000174989.14_14
Transcript (Including UTRs)
   Position: hg19 chr12:117,348,755-117,468,953 Size: 120,199 Total Exon Count: 11 Strand: +
Coding Region
   Position: hg19 chr12:117,348,843-117,465,977 Size: 117,135 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:117,348,755-117,468,953)mRNA (may differ from genome)Protein (598 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: FBXW8_HUMAN
DESCRIPTION: RecName: Full=F-box/WD repeat-containing protein 8; AltName: Full=F-box and WD-40 domain-containing protein 8; AltName: Full=F-box only protein 29;
FUNCTION: Substrate-recognition component of a SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
SUBUNIT: Part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Interacts with CUL7.
INTERACTION: P01106:MYC; NbExp=3; IntAct=EBI-914770, EBI-447544;
SIMILARITY: Contains 1 F-box domain.
SIMILARITY: Contains 5 WD repeats.
SEQUENCE CAUTION: Sequence=AAF03129.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=AAF03129.1; Type=Frameshift; Positions=282, 295;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -43.0088-0.489 Picture PostScript Text
3' UTR -863.102976-0.290 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001810 - F-box_dom_cyclin-like
IPR011047 - Quinonprotein_ADH-like
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR019775 - WD40_repeat_CS
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00646 - F-box domain
PF12937 - F-box-like

SCOP Domains:
81383 - F-box domain
50952 - Soluble quinoprotein glucose dehydrogenase
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
117289 - Nucleoporin domain
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Peptidase/esterase 'gauge' domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - DPP6 N-terminal domain-like
56300 - Metallo-dependent phosphatases

ModBase Predicted Comparative 3D Structure on Q8N3Y1
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding

Biological Process:
GO:0000209 protein polyubiquitination
GO:0007030 Golgi organization
GO:0008283 cell proliferation
GO:0016567 protein ubiquitination
GO:0043687 post-translational protein modification
GO:0050775 positive regulation of dendrite morphogenesis
GO:0060712 spongiotrophoblast layer development
GO:0060716 labyrinthine layer blood vessel development
GO:1901485 positive regulation of transcription factor catabolic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005794 Golgi apparatus
GO:0005829 cytosol
GO:0019005 SCF ubiquitin ligase complex
GO:0031467 Cul7-RING ubiquitin ligase complex
GO:0048471 perinuclear region of cytoplasm
GO:1990393 3M complex


-  Descriptions from all associated GenBank mRNAs
  BC037296 - Homo sapiens F-box and WD repeat domain containing 8, mRNA (cDNA clone MGC:33534 IMAGE:4821240), complete cds.
AK289621 - Homo sapiens cDNA FLJ76330 complete cds, highly similar to Homo sapiens F-box and WD-40 domain protein 8 (FBXW8), transcript variant 2, mRNA.
AF176707 - Homo sapiens F-box protein FBX29 (FBX29) mRNA, partial cds.
BC075823 - Homo sapiens cDNA clone IMAGE:6304781, **** WARNING: chimeric clone ****.
JD496982 - Sequence 478006 from Patent EP1572962.
JD400734 - Sequence 381758 from Patent EP1572962.
JD171851 - Sequence 152875 from Patent EP1572962.
DQ586159 - Homo sapiens piRNA piR-53271, complete sequence.
JD234685 - Sequence 215709 from Patent EP1572962.
JD361766 - Sequence 342790 from Patent EP1572962.
JD042402 - Sequence 23426 from Patent EP1572962.
JD117774 - Sequence 98798 from Patent EP1572962.
JD200530 - Sequence 181554 from Patent EP1572962.
JD291273 - Sequence 272297 from Patent EP1572962.
JD415571 - Sequence 396595 from Patent EP1572962.
JD160642 - Sequence 141666 from Patent EP1572962.
JD224986 - Sequence 206010 from Patent EP1572962.
JD298759 - Sequence 279783 from Patent EP1572962.
JD372670 - Sequence 353694 from Patent EP1572962.
JD237857 - Sequence 218881 from Patent EP1572962.
JD524497 - Sequence 505521 from Patent EP1572962.
AK055849 - Homo sapiens cDNA FLJ31287 fis, clone KIDNE2007186.
JD360733 - Sequence 341757 from Patent EP1572962.
JD239852 - Sequence 220876 from Patent EP1572962.
JD106767 - Sequence 87791 from Patent EP1572962.
JD554023 - Sequence 535047 from Patent EP1572962.
JD480225 - Sequence 461249 from Patent EP1572962.
JD425133 - Sequence 406157 from Patent EP1572962.
JD120450 - Sequence 101474 from Patent EP1572962.
JD068218 - Sequence 49242 from Patent EP1572962.
JD515578 - Sequence 496602 from Patent EP1572962.
JD546554 - Sequence 527578 from Patent EP1572962.
JD492155 - Sequence 473179 from Patent EP1572962.
JD246401 - Sequence 227425 from Patent EP1572962.
JD321316 - Sequence 302340 from Patent EP1572962.
JD213116 - Sequence 194140 from Patent EP1572962.
JD345668 - Sequence 326692 from Patent EP1572962.
JD108085 - Sequence 89109 from Patent EP1572962.
JD342177 - Sequence 323201 from Patent EP1572962.
JD042039 - Sequence 23063 from Patent EP1572962.
JD312111 - Sequence 293135 from Patent EP1572962.
JD346593 - Sequence 327617 from Patent EP1572962.
JD445025 - Sequence 426049 from Patent EP1572962.
JD434019 - Sequence 415043 from Patent EP1572962.
JD109621 - Sequence 90645 from Patent EP1572962.
JD323414 - Sequence 304438 from Patent EP1572962.
JD331544 - Sequence 312568 from Patent EP1572962.
JD160076 - Sequence 141100 from Patent EP1572962.
JD055749 - Sequence 36773 from Patent EP1572962.
JD521858 - Sequence 502882 from Patent EP1572962.
JD403701 - Sequence 384725 from Patent EP1572962.
JD555355 - Sequence 536379 from Patent EP1572962.
JD496607 - Sequence 477631 from Patent EP1572962.
JD418255 - Sequence 399279 from Patent EP1572962.
JD076256 - Sequence 57280 from Patent EP1572962.
JD177003 - Sequence 158027 from Patent EP1572962.
JD249330 - Sequence 230354 from Patent EP1572962.
JD551263 - Sequence 532287 from Patent EP1572962.
JD298172 - Sequence 279196 from Patent EP1572962.
JD289602 - Sequence 270626 from Patent EP1572962.
JD376276 - Sequence 357300 from Patent EP1572962.
JD501667 - Sequence 482691 from Patent EP1572962.
JD316343 - Sequence 297367 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8N3Y1 (Reactome details) participates in the following event(s):

R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-983147 Release of E3 from polyubiquitinated substrate
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-8952620 NEDD8:AcM-UBE2M binds CRL1 E3 ubiquitin ligase complex
R-HSA-8956200 MyrG-DCUN1D3 binds CRL1 E3 ubiquitin ligase complex
R-HSA-8955241 CAND1 binds cytosolic CRL E3 ubiquitin ligases
R-HSA-8952618 AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex
R-HSA-8955289 COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-8956040 COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-8951664 Neddylation
R-HSA-1280218 Adaptive Immune System
R-HSA-597592 Post-translational protein modification
R-HSA-168256 Immune System
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: FBW6, FBW8, FBX29, FBXO29, FBXW6, FBXW8_HUMAN, NM_153348, Q8N3Y1, Q9UK95, uc328uml.1, uc328uml.2
UCSC ID: ENST00000652555.1_4
RefSeq Accession: NM_153348.3
Protein: Q8N3Y1 (aka FBXW8_HUMAN or FBW8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.