Human Gene FGF21 (ENST00000593756.6_4) from GENCODE V47lift37
  Description: fibroblast growth factor 21 (from RefSeq NM_019113.4)
Gencode Transcript: ENST00000593756.6_4
Gencode Gene: ENSG00000105550.10_9
Transcript (Including UTRs)
   Position: hg19 chr19:49,258,781-49,261,587 Size: 2,807 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr19:49,259,494-49,261,477 Size: 1,984 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:49,258,781-49,261,587)mRNA (may differ from genome)Protein (209 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: FGF21_HUMAN
DESCRIPTION: RecName: Full=Fibroblast growth factor 21; Short=FGF-21; Flags: Precursor;
FUNCTION: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
SUBUNIT: Interacts (via C-terminus) with KLB; this interaction is direct. Interacts with FGFR4.
SUBCELLULAR LOCATION: Secreted (Potential).
SIMILARITY: Belongs to the heparin-binding growth factors family.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/fgf21/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: FGF21
Diseases sorted by gene-association score: overnutrition (1), diabetes mellitus, noninsulin-dependent (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.10 RPKM in Liver
Total median expression: 10.28 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -217.40607-0.358 Picture PostScript Text
3' UTR -19.70110-0.179 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008996 - Cytokine_IL1-like
IPR017338 - Fibroblast_GF_15/19/21
IPR002209 - GF_heparin-bd
IPR002348 - IL1_HBGF

Pfam Domains:
PF00167 - Fibroblast growth factor

SCOP Domains:
50353 - Cytokine
50405 - Actin-crosslinking proteins

ModBase Predicted Comparative 3D Structure on Q9NSA1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005104 fibroblast growth factor receptor binding
GO:0005515 protein binding
GO:0008083 growth factor activity

Biological Process:
GO:0007165 signal transduction
GO:0007267 cell-cell signaling
GO:0008284 positive regulation of cell proliferation
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0010469 regulation of receptor activity
GO:0010898 positive regulation of triglyceride catabolic process
GO:0010988 regulation of low-density lipoprotein particle clearance
GO:0014823 response to activity
GO:0030968 endoplasmic reticulum unfolded protein response
GO:0031667 response to nutrient levels
GO:0035690 cellular response to drug
GO:0045716 positive regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0046326 positive regulation of glucose import
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0071333 cellular response to glucose stimulus
GO:0071377 cellular response to glucagon stimulus
GO:0071404 cellular response to low-density lipoprotein particle stimulus
GO:0072577 endothelial cell apoptotic process
GO:0090080 positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway
GO:1901215 negative regulation of neuron death
GO:1904640 response to methionine
GO:2000352 negative regulation of endothelial cell apoptotic process

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005623 cell


-  Descriptions from all associated GenBank mRNAs
  BC018404 - Homo sapiens fibroblast growth factor 21, mRNA (cDNA clone MGC:21502 IMAGE:3881069), complete cds.
AY359086 - Homo sapiens clone DNA144841 FGF21 (UNQ3115) mRNA, complete cds.
AB590410 - Synthetic construct DNA, clone: pFN21AE1420, Homo sapiens FGF21 gene for fibroblast growth factor 21, without stop codon, in Flexi system.
DQ891786 - Synthetic construct clone IMAGE:100004416; FLH179904.01X; RZPDo839D10132D fibroblast growth factor 21 (FGF21) gene, encodes complete protein.
DQ894970 - Synthetic construct Homo sapiens clone IMAGE:100009430; FLH179900.01L; RZPDo839D10131D fibroblast growth factor 21 (FGF21) gene, encodes complete protein.
AB021975 - Homo sapiens mRNA for FGF-21, complete cds.
FZ414866 - WO 2011010706-A/26: FGF21 cis-element binding substance.
JC201587 - Sequence 26 from Patent EP2458005.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9NSA1 (Reactome details) participates in the following event(s):

R-HSA-8963889 Assembly of active LPL and LIPC lipase complexes
R-HSA-8963899 Plasma lipoprotein remodeling
R-HSA-174824 Plasma lipoprotein assembly, remodeling, and clearance
R-HSA-382551 Transport of small molecules

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000593756.1, ENST00000593756.2, ENST00000593756.3, ENST00000593756.4, ENST00000593756.5, FGF21_HUMAN, NM_019113, Q8N683, Q9NSA1, uc326zbg.1, uc326zbg.2, UNQ3115/PRO10196
UCSC ID: ENST00000593756.6_4
RefSeq Accession: NM_019113.4
Protein: Q9NSA1 (aka FGF21_HUMAN or FGFL_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.