Human Gene FHL2 (ENST00000530340.6_8) from GENCODE V47lift37
  Description: four and a half LIM domains 2, transcript variant 10 (from RefSeq NM_001318898.2)
Gencode Transcript: ENST00000530340.6_8
Gencode Gene: ENSG00000115641.19_12
Transcript (Including UTRs)
   Position: hg19 chr2:105,977,283-106,015,508 Size: 38,226 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr2:105,977,740-106,002,973 Size: 25,234 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:105,977,283-106,015,508)mRNA (may differ from genome)Protein (279 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: Q6I9R8_HUMAN
DESCRIPTION: SubName: Full=FHL2 protein; SubName: Full=Four and a half LIM domains 2; SubName: Full=HCG28013, isoform CRA_a; SubName: Full=cDNA, FLJ94468, Homo sapiens four and a half LIM domains 2 (FHL2), mRNA;
SIMILARITY: Contains 4 LIM zinc-binding domains.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: FHL2
Diseases sorted by gene-association score: familial isolated dilated cardiomyopathy* (58), rhabdomyosarcoma (19), hemophagocytic lymphohistiocytosis (14), hypogonadotropic hypogonadism 24 without anosmia (11), hemophagocytic lymphohistiocytosis, familial, 4 (9), cervical polyp (8), testis seminoma (7)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 133.41 RPKM in Heart - Left Ventricle
Total median expression: 597.20 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -126.60285-0.444 Picture PostScript Text
3' UTR -96.20457-0.211 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001781 - Znf_LIM

Pfam Domains:
PF00412 - LIM domain

SCOP Domains:
48695 - Multiheme cytochromes
57716 - Glucocorticoid receptor-like (DNA-binding domain)

ModBase Predicted Comparative 3D Structure on Q6I9R8
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0046872 metal ion binding

Biological Process:
GO:0001649 osteoblast differentiation
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0045892 negative regulation of transcription, DNA-templated
GO:0055014 atrial cardiac muscle cell development
GO:0055015 ventricular cardiac muscle cell development
GO:0060347 heart trabecula formation

Cellular Component:
GO:0005634 nucleus
GO:0030018 Z disc
GO:0031430 M band


-  Descriptions from all associated GenBank mRNAs
  AB158503 - Homo sapiens FHL2 mRNA for splice variant of four and a half LIM domain protein 2, complete cds.
U29332 - Homo sapiens heart protein (FHL-2) mRNA, complete cds.
BC036085 - Homo sapiens four and a half LIM domains 2, mRNA (cDNA clone IMAGE:5298723).
CR936682 - Homo sapiens mRNA; cDNA DKFZp781J01103 (from clone DKFZp781J01103).
BC093049 - Homo sapiens four and a half LIM domains 2, mRNA (cDNA clone MGC:111118 IMAGE:30374919), complete cds.
BC012742 - Homo sapiens four and a half LIM domains 2, mRNA (cDNA clone MGC:15296 IMAGE:3840517), complete cds.
BC014397 - Homo sapiens four and a half LIM domains 2, mRNA (cDNA clone MGC:1574 IMAGE:3546686), complete cds.
DQ266432 - Homo sapiens FHL2 isoform 5 mRNA, complete cds.
AK097756 - Homo sapiens cDNA FLJ40437 fis, clone TESTI2039707.
CR936799 - Homo sapiens mRNA; cDNA DKFZp781D198 (from clone DKFZp781D198).
L42176 - Homo sapiens (clone 35.3) DRAL mRNA, complete cds.
JD357328 - Sequence 338352 from Patent EP1572962.
JD227979 - Sequence 209003 from Patent EP1572962.
JD306819 - Sequence 287843 from Patent EP1572962.
U60117 - Human skeletal muscle LIM-protein SLIM3 mRNA, partial cds.
JD086982 - Sequence 68006 from Patent EP1572962.
JD363259 - Sequence 344283 from Patent EP1572962.
JD556934 - Sequence 537958 from Patent EP1572962.
JD312769 - Sequence 293793 from Patent EP1572962.
JD316870 - Sequence 297894 from Patent EP1572962.
AY634685 - Homo sapiens aging-associated gene 11 (AAG11) mRNA, complete cds.
DQ307067 - Homo sapiens bone marrow four and a half LIM-domain protein 2 (FHL2) mRNA, complete cds.
JQ313902 - Homo sapiens four-and-a-half LIM domains 2 (FHL2) mRNA, complete cds.
AK313841 - Homo sapiens cDNA, FLJ94468, Homo sapiens four and a half LIM domains 2 (FHL2), mRNA.
CU676975 - Synthetic construct Homo sapiens gateway clone IMAGE:100023317 5' read FHL2 mRNA.
KJ891174 - Synthetic construct Homo sapiens clone ccsbBroadEn_00568 FHL2 gene, encodes complete protein.
BT006960 - Homo sapiens four and a half LIM domains 2 mRNA, complete cds.
DQ896335 - Synthetic construct Homo sapiens clone IMAGE:100010795; FLH193378.01L; RZPDo839F0868D four and a half LIM domains 2 (FHL2) gene, encodes complete protein.
EU176424 - Synthetic construct Homo sapiens clone IMAGE:100006502; FLH193382.01X; RZPDo839F0878D four and a half LIM domains 2 (FHL2) gene, encodes complete protein.
AB590239 - Synthetic construct DNA, clone: pFN21AB6530, Homo sapiens FHL2 gene for four and a half LIM domains 2, without stop codon, in Flexi system.
CR457437 - Homo sapiens full open reading frame cDNA clone RZPDo834D076D for gene FHL2, four and a half LIM domains 2; complete cds, incl. stopcodon.
JD564241 - Sequence 545265 from Patent EP1572962.
JD121013 - Sequence 102037 from Patent EP1572962.
JD121012 - Sequence 102036 from Patent EP1572962.
JD069230 - Sequence 50254 from Patent EP1572962.
JD200511 - Sequence 181535 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_p53hypoxiaPathway - Hypoxia and p53 in the Cardiovascular system

-  Other Names for This Gene
  Alternate Gene Symbols: D3DVK0, ENST00000530340.1, ENST00000530340.2, ENST00000530340.3, ENST00000530340.4, ENST00000530340.5, FHL2 , hCG_28013 , NM_001318898, Q561W1, Q6I9R8, Q6I9R8_HUMAN, uc324hhj.1, uc324hhj.2
UCSC ID: ENST00000530340.6_8
RefSeq Accession: NM_001318895.3
Protein: Q6I9R8

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.