Human Gene GAGE2D (ENST00000404720.2) from GENCODE V47lift37
  Description: G antigen 8 (from RefSeq NM_012196.1)
Gencode Transcript: ENST00000404720.2
Gencode Gene: ENSG00000240257.2
Transcript (Including UTRs)
   Position: hg19 chrX:49,207,160-49,214,420 Size: 7,261 Total Exon Count: 5 Strand: +
Coding Region
   Position: hg19 chrX:49,208,272-49,214,326 Size: 6,055 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:49,207,160-49,214,420)mRNA (may differ from genome)Protein (116 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GGE2D_HUMAN
DESCRIPTION: RecName: Full=G antigen 2D; Short=GAGE-2D; AltName: Full=Cancer/testis antigen 4.8; Short=CT4.8; AltName: Full=G antigen 8; Short=GAGE-8;
INTERACTION: Q8NEA9:GMCL1P1; NbExp=2; IntAct=EBI-751641, EBI-745707;
TISSUE SPECIFICITY: Not expressed in normal tissues, except in testis, but expressed by a large proportion of tumors of various histological origins.
MISCELLANEOUS: This gene belongs to a family of genes organized in clustered repeats. They have a high degree of predicted sequence identity, but differ by scattered single nucleotide substitution. Their sequences contain either the antigenic peptide YYWPRPRRY or YRPRPRRY which is recognized by cytotoxic T-cells.
SIMILARITY: Belongs to the GAGE family.
CAUTION: The first GAGE nomenclature was based on identified mRNA sequences, but the high identity of the GAGE members made impossible to separate products of paralogous genes from polymorph products. PubMed:18179644 presented a new GAGE gene nomenclature based on the identified genes and their products.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 64.64 RPKM in Testis
Total median expression: 64.96 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -16.8072-0.233 Picture PostScript Text
3' UTR -18.9094-0.201 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008625 - GAGE

Pfam Domains:
PF05831 - GAGE protein

ModBase Predicted Comparative 3D Structure on Q9UEU5
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0006968 cellular defense response


-  Descriptions from all associated GenBank mRNAs
  U19143 - Human GAGE-2 protein mRNA, complete cds.
BC069309 - Homo sapiens G antigen 2A, mRNA (cDNA clone MGC:96930 IMAGE:7262139), complete cds.
BC069397 - Homo sapiens G antigen 2A, mRNA (cDNA clone MGC:96942 IMAGE:7262151), complete cds.
BC069558 - Homo sapiens G antigen 2A, mRNA (cDNA clone MGC:96883 IMAGE:7262092), complete cds.
BC024914 - Homo sapiens G antigen 5, mRNA (cDNA clone MGC:21275 IMAGE:4471969), complete cds.
U19145 - Human GAGE-4 protein mRNA, complete cds.
U19146 - Human GAGE-5 protein mRNA, complete cds.
BC171769 - Homo sapiens G antigen 4, mRNA (cDNA clone MGC:198484 IMAGE:9054423), complete cds.
BC018052 - Homo sapiens G antigen 2E, mRNA (cDNA clone MGC:26395 IMAGE:4812462), complete cds.
BC113926 - Homo sapiens G antigen 2C, mRNA (cDNA clone MGC:120097 IMAGE:40021787), complete cds.
AB590984 - Synthetic construct DNA, clone: pFN21AE1110, Homo sapiens GAGE2E gene for G antigen 2E, without stop codon, in Flexi system.
BC156222 - Synthetic construct Homo sapiens clone IMAGE:100061614, MGC:190052 G antigen 8 (GAGE8) mRNA, encodes complete protein.
KJ893578 - Synthetic construct Homo sapiens clone ccsbBroadEn_02972 GAGE2E gene, encodes complete protein.
BC156971 - Synthetic construct Homo sapiens clone IMAGE:100063587, MGC:190826 G antigen 8 (GAGE8) mRNA, encodes complete protein.
DQ579008 - Homo sapiens piRNA piR-47120, complete sequence.
JD263734 - Sequence 244758 from Patent EP1572962.
JD502771 - Sequence 483795 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A6NG46, A6NNR8, B7ZL76, ENST00000404720.1, GAGE8, GGE2D_HUMAN, NM_012196, Q4V325, Q9UEU5, uc319csw.1
UCSC ID: ENST00000404720.2
RefSeq Accession: NM_001098407.1
Protein: Q9UEU5 (aka GGE2D_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.