Human Gene GID8 (ENST00000266069.5_4) from GENCODE V47lift37
  Description: GID complex subunit 8 homolog (from RefSeq NM_017896.3)
Gencode Transcript: ENST00000266069.5_4
Gencode Gene: ENSG00000101193.8_7
Transcript (Including UTRs)
   Position: hg19 chr20:61,569,499-61,579,827 Size: 10,329 Total Exon Count: 5 Strand: +
Coding Region
   Position: hg19 chr20:61,572,855-61,576,264 Size: 3,410 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr20:61,569,499-61,579,827)mRNA (may differ from genome)Protein (228 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GID8_HUMAN
DESCRIPTION: RecName: Full=Glucose-induced degradation protein 8 homolog; AltName: Full=Two hybrid-associated protein 1 with RanBPM; Short=Twa1;
SUBUNIT: Interacts with RANBP9. Part of a complex consisting of RANBP9, MKLN1 and GID8.
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: Belongs to the GID8 family.
SIMILARITY: Contains 1 CTLH domain.
SIMILARITY: Contains 1 LisH domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 43.95 RPKM in Esophagus - Mucosa
Total median expression: 892.99 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -50.10119-0.421 Picture PostScript Text
3' UTR -1228.803563-0.345 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013144 - CRA_dom
IPR024964 - CTLH/CRA
IPR006595 - CTLH_C
IPR006594 - LisH_dimerisation
IPR013720 - LisH_dimerisation_subgr

Pfam Domains:
PF08513 - LisH
PF10607 - CTLH/CRA C-terminal to LisH motif domain

ModBase Predicted Comparative 3D Structure on Q9NWU2
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0030054 cell junction


-  Descriptions from all associated GenBank mRNAs
  BC032120 - Homo sapiens chromosome 20 open reading frame 11, mRNA (cDNA clone MGC:29516 IMAGE:4137113), complete cds.
AK299509 - Homo sapiens cDNA FLJ52229 complete cds.
AK000609 - Homo sapiens cDNA FLJ20602 fis, clone KAT07189.
JD390059 - Sequence 371083 from Patent EP1572962.
JD395111 - Sequence 376135 from Patent EP1572962.
JD132660 - Sequence 113684 from Patent EP1572962.
JD253044 - Sequence 234068 from Patent EP1572962.
JD462890 - Sequence 443914 from Patent EP1572962.
CU689278 - Synthetic construct Homo sapiens gateway clone IMAGE:100021884 5' read C20orf11 mRNA.
HQ447747 - Synthetic construct Homo sapiens clone IMAGE:100071078; CCSB012577_01 chromosome 20 open reading frame 11 (C20orf11) gene, encodes complete protein.
KJ899051 - Synthetic construct Homo sapiens clone ccsbBroadEn_08445 C20orf11 gene, encodes complete protein.
DQ582311 - Homo sapiens piRNA piR-32423, complete sequence.
JD144390 - Sequence 125414 from Patent EP1572962.
JD277067 - Sequence 258091 from Patent EP1572962.
JD277068 - Sequence 258092 from Patent EP1572962.
JD131659 - Sequence 112683 from Patent EP1572962.
JD541818 - Sequence 522842 from Patent EP1572962.
DQ601588 - Homo sapiens piRNA piR-39654, complete sequence.
JD037587 - Sequence 18611 from Patent EP1572962.
JD565525 - Sequence 546549 from Patent EP1572962.
JD080372 - Sequence 61396 from Patent EP1572962.
JD353764 - Sequence 334788 from Patent EP1572962.
JD186318 - Sequence 167342 from Patent EP1572962.
JD408994 - Sequence 390018 from Patent EP1572962.
JD155037 - Sequence 136061 from Patent EP1572962.
JD289351 - Sequence 270375 from Patent EP1572962.
JD452198 - Sequence 433222 from Patent EP1572962.
JD130193 - Sequence 111217 from Patent EP1572962.
JD432723 - Sequence 413747 from Patent EP1572962.
JD063263 - Sequence 44287 from Patent EP1572962.
LF206064 - JP 2014500723-A/13567: Polycomb-Associated Non-Coding RNAs.
AK025775 - Homo sapiens cDNA: FLJ22122 fis, clone HEP19214.
JD067961 - Sequence 48985 from Patent EP1572962.
JD527305 - Sequence 508329 from Patent EP1572962.
JD222771 - Sequence 203795 from Patent EP1572962.
JD492945 - Sequence 473969 from Patent EP1572962.
JD172769 - Sequence 153793 from Patent EP1572962.
BC031819 - Homo sapiens chromosome 20 open reading frame 11, mRNA (cDNA clone IMAGE:4454305), partial cds.
JD549321 - Sequence 530345 from Patent EP1572962.
JD422974 - Sequence 403998 from Patent EP1572962.
JD555638 - Sequence 536662 from Patent EP1572962.
JD310670 - Sequence 291694 from Patent EP1572962.
JD167701 - Sequence 148725 from Patent EP1572962.
JD409583 - Sequence 390607 from Patent EP1572962.
LF350948 - JP 2014500723-A/158451: Polycomb-Associated Non-Coding RNAs.
JD440474 - Sequence 421498 from Patent EP1572962.
JD436640 - Sequence 417664 from Patent EP1572962.
JD546604 - Sequence 527628 from Patent EP1572962.
JD269824 - Sequence 250848 from Patent EP1572962.
JD402366 - Sequence 383390 from Patent EP1572962.
JD269825 - Sequence 250849 from Patent EP1572962.
JD522023 - Sequence 503047 from Patent EP1572962.
JD563040 - Sequence 544064 from Patent EP1572962.
JD303045 - Sequence 284069 from Patent EP1572962.
JD168524 - Sequence 149548 from Patent EP1572962.
JD223351 - Sequence 204375 from Patent EP1572962.
JD545967 - Sequence 526991 from Patent EP1572962.
JD144922 - Sequence 125946 from Patent EP1572962.
JD493267 - Sequence 474291 from Patent EP1572962.
JD141294 - Sequence 122318 from Patent EP1572962.
JD372766 - Sequence 353790 from Patent EP1572962.
JD260005 - Sequence 241029 from Patent EP1572962.
JD154607 - Sequence 135631 from Patent EP1572962.
JD292107 - Sequence 273131 from Patent EP1572962.
JD226222 - Sequence 207246 from Patent EP1572962.
JD091049 - Sequence 72073 from Patent EP1572962.
JD244810 - Sequence 225834 from Patent EP1572962.
JD244184 - Sequence 225208 from Patent EP1572962.
MA586525 - JP 2018138019-A/158451: Polycomb-Associated Non-Coding RNAs.
MA441641 - JP 2018138019-A/13567: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: C20orf11, E1P5I3, ENST00000266069.1, ENST00000266069.2, ENST00000266069.3, ENST00000266069.4, GID8_HUMAN, NM_017896, Q8N5M5, Q9NWU2, TWA1 , uc317igd.1, uc317igd.2
UCSC ID: ENST00000266069.5_4
RefSeq Accession: NM_017896.3
Protein: Q9NWU2 (aka GID8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.