Human Gene GIT2 (ENST00000355312.8_9) from GENCODE V47lift37

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Description: GIT ArfGAP 2, transcript variant 1 (from RefSeq NM_057169.5)
Gencode Transcript: ENST00000355312.8_9
Gencode Gene: ENSG00000139436.22_14
Transcript (Including UTRs)
Position: hg19 chr12:110,367,609-110,434,173 Size: 66,565 Total Exon Count: 20 Strand: -
Coding Region
Position: hg19 chr12:110,370,783-110,434,029 Size: 63,247 Coding Exon Count: 20

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species	GO Annotations
mRNA Descriptions	Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr12:110,367,609-110,434,173)			mRNA (may differ from genome)		Protein (759 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	MGI	OMIM	PubMed	UniProtKB	Wikipedia
BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: GIT2_HUMAN DESCRIPTION: RecName: Full=ARF GTPase-activating protein GIT2; Short=ARF GAP GIT2; AltName: Full=Cool-interacting tyrosine-phosphorylated protein 2; Short=CAT-2; Short=CAT2; AltName: Full=G protein-coupled receptor kinase-interactor 2; AltName: Full=GRK-interacting protein 2; FUNCTION: GTPase-activating protein for the ADP ribosylation factor family. SUBUNIT: Interacts with TGFB1I1 (By similarity). Interacts with G protein-coupled receptor kinases. Associates with paxillin. Also interacts with PIX exchange factors. INTERACTION: Q9ES28:Arhgef7 (xeno); NbExp=2; IntAct=EBI-1046878, EBI-642580; Q9Y6K9:IKBKG; NbExp=6; IntAct=EBI-1046878, EBI-81279; Q15025:TNIP1; NbExp=3; IntAct=EBI-1046878, EBI-357849; Q13077:TRAF1; NbExp=2; IntAct=EBI-1046878, EBI-359224; Q15631:TSN; NbExp=2; IntAct=EBI-1046878, EBI-1044160; SIMILARITY: Contains 3 ANK repeats. SIMILARITY: Contains 1 Arf-GAP domain. SEQUENCE CAUTION: Sequence=BAA09769.2; Type=Erroneous initiation;

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl D015085 2,4,5-Trichlorophenoxyacetic Acid C023514 2,6-dinitrotoluene D015123 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide D000082 Acetaminophen D016604 Aflatoxin B1 D001564 Benzo(a)pyrene D019327 Copper Sulfate D004997 Ethinyl Estradiol D007854 Lead D018817 N-Methyl-3,4-methylenedioxyamphetamine D012906 Smoke D012999 Soman D013629 Tamoxifen D013749 Tetrachlorodibenzodioxin D014303 Trinitrotoluene D014635 Valproic Acid C006632 arsenic trioxide C014347 decitabine C045651 epigallocatechin gallate C039281 furan C006253 pirinixic acid C027373 potassium chromate(VI) C059514 resveratrol

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 11.89 RPKM in Whole Blood Total median expression: 227.65 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-66.30	144	-0.460	Picture	PostScript	Text
3' UTR	-876.70	3174	-0.276	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR002110 - Ankyrin_rpt
IPR020683 - Ankyrin_rpt-contain_dom
IPR001164 - ArfGAP
IPR022018 - GIT1_C
IPR013724 - GIT_SHD

Pfam Domains:
PF00023 - Ankyrin repeat
PF01412 - Putative GTPase activating protein for Arf
PF08518 - Spa2 homology domain (SHD) of GIT
PF12205 - G protein-coupled receptor kinase-interacting protein 1 C term
PF12796 - Ankyrin repeats (3 copies)
PF13857 - Ankyrin repeats (many copies)
PF16559 - GIT coiled-coil Rho guanine nucleotide exchange factor

SCOP Domains:
140860 - Pseudo ankyrin repeat-like
46785 - "Winged helix" DNA-binding domain
48403 - Ankyrin repeat
57863 - ArfGap/RecO-like zinc finger

ModBase Predicted Comparative 3D Structure on Q14161


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details	Gene Details
Gene Sorter	Gene Sorter
	RGD

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0005096 GTPase activator activity GO:0005515 protein binding GO:0046872 metal ion binding Biological Process: GO:0008277 regulation of G-protein coupled receptor protein signaling pathway GO:0043547 positive regulation of GTPase activity Cellular Component: GO:0005654 nucleoplasm GO:0005925 focal adhesion

Descriptions from all associated GenBank mRNAs


	BC039880 - Homo sapiens G protein-coupled receptor kinase interactor 2, mRNA (cDNA clone IMAGE:4524415), partial cds. AK091325 - Homo sapiens cDNA FLJ34006 fis, clone FCBBF1000294. AK090652 - Homo sapiens cDNA FLJ33333 fis, clone BRACE2000676. CR749340 - Homo sapiens mRNA; cDNA DKFZp686G01261 (from clone DKFZp686G01261). D63482 - Homo sapiens mRNA for KIAA0148 gene. AK057814 - Homo sapiens cDNA FLJ25085 fis, clone CBL08588. JD503950 - Sequence 484974 from Patent EP1572962. JD066996 - Sequence 48020 from Patent EP1572962. JD256962 - Sequence 237986 from Patent EP1572962. JD253153 - Sequence 234177 from Patent EP1572962. JD163967 - Sequence 144991 from Patent EP1572962. JD561956 - Sequence 542980 from Patent EP1572962. JD271774 - Sequence 252798 from Patent EP1572962. JD248370 - Sequence 229394 from Patent EP1572962. JD152906 - Sequence 133930 from Patent EP1572962. JD532748 - Sequence 513772 from Patent EP1572962. JD403678 - Sequence 384702 from Patent EP1572962. JD487380 - Sequence 468404 from Patent EP1572962. JD433450 - Sequence 414474 from Patent EP1572962. JD449596 - Sequence 430620 from Patent EP1572962. JD135657 - Sequence 116681 from Patent EP1572962. JD078950 - Sequence 59974 from Patent EP1572962. JD474606 - Sequence 455630 from Patent EP1572962. JD229158 - Sequence 210182 from Patent EP1572962. JD429141 - Sequence 410165 from Patent EP1572962. JD379080 - Sequence 360104 from Patent EP1572962. JD229950 - Sequence 210974 from Patent EP1572962. JD382012 - Sequence 363036 from Patent EP1572962. JD134691 - Sequence 115715 from Patent EP1572962. JD519309 - Sequence 500333 from Patent EP1572962. JD116481 - Sequence 97505 from Patent EP1572962. JD513107 - Sequence 494131 from Patent EP1572962. JD196277 - Sequence 177301 from Patent EP1572962. BC014223 - Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2, mRNA (cDNA clone IMAGE:4336368), partial cds. JD095768 - Sequence 76792 from Patent EP1572962. JD286520 - Sequence 267544 from Patent EP1572962. JD296765 - Sequence 277789 from Patent EP1572962. AF124491 - Homo sapiens ARF GTPase-activating protein GIT2 (KIAA0148) mRNA, complete cds. KJ902024 - Synthetic construct Homo sapiens clone ccsbBroadEn_11418 GIT2 gene, encodes complete protein. AB385285 - Synthetic construct DNA, clone: pF1KA0148, Homo sapiens GIT2 gene for ARF GTPase-activating protein GIT2, complete cds, without stop codon, in Flexi system. AK303198 - Homo sapiens cDNA FLJ56041 complete cds, highly similar to ARF GTPase-activating protein GIT2. AK308304 - Homo sapiens cDNA, FLJ98252. BC001379 - Homo sapiens G protein-coupled receptor kinase interacting ArfGAP 2, mRNA (cDNA clone MGC:760 IMAGE:3051069), complete cds. AK222736 - Homo sapiens mRNA for G protein-coupled receptor kinase-interactor 2 isoform 4 variant, clone: DMC03310. AK125441 - Homo sapiens cDNA FLJ43452 fis, clone OCBBF2033948, highly similar to Homo sapiens G protein-coupled receptor kinase-interactor 2 (GIT2). CR533568 - Homo sapiens full open reading frame cDNA clone RZPDo834B0122D for gene GIT2, G protein-coupled receptor kinase-interactor 2; complete cds, incl. stopcodon. AB451294 - Homo sapiens GIT2 mRNA for G protein-coupled receptor kinase-interactor 2 isoform 4, complete cds, clone: FLJ08143AAAN. BT007312 - Homo sapiens G protein-coupled receptor kinase-interactor 2 mRNA, complete cds. AB451428 - Homo sapiens GIT2 mRNA for G protein-coupled receptor kinase-interactor 2 isoform 4, partial cds, clone: FLJ08143AAAF. AK304240 - Homo sapiens cDNA FLJ58348 complete cds, highly similar to ARF GTPase-activating protein GIT2. CU676935 - Synthetic construct Homo sapiens gateway clone IMAGE:100019832 5' read GIT2 mRNA.

Biochemical and Signaling Pathways


	BioCarta from NCI Cancer Genome Anatomy Project h_agrPathway - Agrin in Postsynaptic Differentiation

Other Names for This Gene


	Alternate Gene Symbols: ENST00000355312.1, ENST00000355312.2, ENST00000355312.3, ENST00000355312.4, ENST00000355312.5, ENST00000355312.6, ENST00000355312.7, GIT2_HUMAN, KIAA0148, NM_057169, Q14161, Q86U59, Q96CI2, Q9BV91, Q9Y5V2, uc317zan.1, uc317zan.2 UCSC ID: ENST00000355312.8_9 RefSeq Accession: NM_057169.5 Protein: Q14161 (aka GIT2_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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