Human Gene GNA14 (ENST00000341700.7_7) from GENCODE V47lift37
  Description: G protein subunit alpha 14 (from RefSeq NM_004297.4)
Gencode Transcript: ENST00000341700.7_7
Gencode Gene: ENSG00000156049.7_9
Transcript (Including UTRs)
   Position: hg19 chr9:80,037,995-80,263,238 Size: 225,244 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr9:80,038,895-80,262,709 Size: 223,815 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:80,037,995-80,263,238)mRNA (may differ from genome)Protein (355 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GNA14_HUMAN
DESCRIPTION: RecName: Full=Guanine nucleotide-binding protein subunit alpha-14; Short=G alpha-14; Short=G-protein subunit alpha-14;
FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
SIMILARITY: Belongs to the G-alpha family. G(q) subfamily.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: GNA14
Diseases sorted by gene-association score: angioma, tufted* (350), kaposiform hemangioendothelioma* (350), pertussis (46), choreoacanthocytosis (9)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.09 RPKM in Brain - Spinal cord (cervical c-1)
Total median expression: 124.30 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -232.90529-0.440 Picture PostScript Text
3' UTR -207.20900-0.230 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000654 - Gprotein_alpha_Q
IPR001019 - Gprotein_alpha_su
IPR011025 - GproteinA_insert

Pfam Domains:
PF00025 - ADP-ribosylation factor family
PF00503 - G-protein alpha subunit

SCOP Domains:
47895 - Transducin (alpha subunit), insertion domain
49503 - Cupredoxins
52540 - P-loop containing nucleoside triphosphate hydrolases

ModBase Predicted Comparative 3D Structure on O95837
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0001664 G-protein coupled receptor binding
GO:0003924 GTPase activity
GO:0004871 signal transducer activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0019001 guanyl nucleotide binding
GO:0031683 G-protein beta/gamma-subunit complex binding
GO:0046872 metal ion binding

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007188 adenylate cyclase-modulating G-protein coupled receptor signaling pathway
GO:0030168 platelet activation
GO:0060158 phospholipase C-activating dopamine receptor signaling pathway

Cellular Component:
GO:0005834 heterotrimeric G-protein complex
GO:0005886 plasma membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AF105201 - Homo sapiens G-protein alpha subunit 14 (Galpha14) mRNA, complete cds.
BC027886 - Homo sapiens guanine nucleotide binding protein (G protein), alpha 14, mRNA (cDNA clone MGC:34487 IMAGE:5221889), complete cds.
AF493903 - Homo sapiens guanine nucleotide binding protein alpha 14 (GNA14) mRNA, complete cds.
AK312460 - Homo sapiens cDNA, FLJ92815, Homo sapiens guanine nucleotide binding protein (G protein), alpha 14 (GNA14), mRNA.
KJ905380 - Synthetic construct Homo sapiens clone ccsbBroadEn_14942 GNA14 gene, encodes complete protein.
DQ890949 - Synthetic construct Homo sapiens clone IMAGE:100003579; FLH263577.01X; RZPDo839G0890D guanine nucleotide binding protein (G protein), alpha 14 (GNA14) gene, encodes complete protein.
DQ890954 - Synthetic construct clone IMAGE:100003584; FLH167925.01X; RZPDo839H0490D guanine nucleotide binding protein (G protein), alpha 14 (GNA14) gene, encodes complete protein.
EU176237 - Synthetic construct Homo sapiens clone IMAGE:100006620; FLH264081.01X; RZPDo839B11257D guanine nucleotide binding protein (G protein), alpha 14 (GNA14) gene, encodes complete protein.
DQ894116 - Synthetic construct Homo sapiens clone IMAGE:100008576; FLH167921.01L; RZPDo839H0489D guanine nucleotide binding protein (G protein), alpha 14 (GNA14) gene, encodes complete protein.
JD563708 - Sequence 544732 from Patent EP1572962.
JD332503 - Sequence 313527 from Patent EP1572962.
JD507748 - Sequence 488772 from Patent EP1572962.
JD292237 - Sequence 273261 from Patent EP1572962.
JD129673 - Sequence 110697 from Patent EP1572962.
JD459073 - Sequence 440097 from Patent EP1572962.
JD390207 - Sequence 371231 from Patent EP1572962.
JD129225 - Sequence 110249 from Patent EP1572962.
JD071586 - Sequence 52610 from Patent EP1572962.
JD523152 - Sequence 504176 from Patent EP1572962.
JD210024 - Sequence 191048 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O95837 (Reactome details) participates in the following event(s):

R-HSA-398188 PLC beta is activated by G alpha (q)
R-HSA-400586 G alpha (q) binds to Trio family RhoGEFs
R-HSA-416358 G alpha (q) inhibits PI3K alpha
R-HSA-416510 GRK5 sequesters activated Gq
R-HSA-416516 GRK2 sequesters activated Gq
R-HSA-418582 G alpha (q) auto-inactivates by hydrolysing GTP to GDP
R-HSA-397835 Dissociation of the PAR:Gq complex
R-HSA-418576 Dissociation of the P2Y purinoceptor 1:Gq complex
R-HSA-428752 Dissociation of the TP:Gq complex
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-750993 Inactive G alpha (q) reassociates with G beta:gamma
R-HSA-400023 Gq alpha activates Phospholipase C beta
R-HSA-400027 Gq alpha:G beta:G gamma dissociates to Gq alpha:GTP and G beta:G gamma
R-HSA-396996 Thrombin-activated PAR binds G-protein Gq
R-HSA-418581 Activated P2Y purinoceptor 1 binds G-protein Gq
R-HSA-428749 Activated TP receptor binds G-proten Gq
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-399995 Muscarinic Acetylcholine Receptor M3 activates Gq
R-HSA-416530 GPR40:fatty acid activates Gq
R-HSA-114558 Gq activation by PAR
R-HSA-418579 Gq activation by P2Y purinoceptor 1
R-HSA-428750 Gq activation by TP receptor
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-114688 PLC-beta hydrolyses PIP2 to DAG and IP3
R-HSA-399998 Activated Phospholipase C beta-1 hydrolyzes 1-Phosphatidyl-D-myo-inositol 4,5-bisphosphate
R-HSA-416476 G alpha (q) signalling events
R-HSA-456926 Thrombin signalling through proteinase activated receptors (PARs)
R-HSA-418592 ADP signalling through P2Y purinoceptor 1
R-HSA-428930 Thromboxane signalling through TP receptor
R-HSA-399997 Acetylcholine regulates insulin secretion
R-HSA-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-HSA-388396 GPCR downstream signalling
R-HSA-76002 Platelet activation, signaling and aggregation
R-HSA-392518 Signal amplification
R-HSA-422356 Regulation of insulin secretion
R-HSA-400451 Free fatty acids regulate insulin secretion
R-HSA-372790 Signaling by GPCR
R-HSA-109582 Hemostasis
R-HSA-163685 Energy Metabolism
R-HSA-162582 Signal Transduction
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: B1ALW3, ENST00000341700.1, ENST00000341700.2, ENST00000341700.3, ENST00000341700.4, ENST00000341700.5, ENST00000341700.6, GNA14_HUMAN, NM_004297, O95837, uc317wcn.1, uc317wcn.2
UCSC ID: ENST00000341700.7_7
RefSeq Accession: NM_004297.4
Protein: O95837 (aka GNA14_HUMAN or GB14_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.