ID:GNAI2_HUMAN DESCRIPTION: RecName: Full=Guanine nucleotide-binding protein G(i) subunit alpha-2; AltName: Full=Adenylate cyclase-inhibiting G alpha protein; FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. FUNCTION: Isoform sGi2: Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool. SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1. Interacts with RGS12 and RGS14 (By similarity). Interacts with UNC5B. INTERACTION: Q99750:MDFI; NbExp=2; IntAct=EBI-353997, EBI-724076; SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Cell membrane. Note=Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. SIMILARITY: Belongs to the G-alpha family. G(i/o/t/z) subfamily.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P04899
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK096595 - Homo sapiens cDNA FLJ39276 fis, clone OCBBF2010843, highly similar to Guanine nucleotide-binding protein G(i), alpha-2 subunit. BC016995 - Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, mRNA (cDNA clone MGC:22031 IMAGE:4403631), complete cds. AK096299 - Homo sapiens cDNA FLJ38980 fis, clone NT2RI2004884, highly similar to Guanine nucleotide-binding protein G(i), alpha-2 subunit. AK126708 - Homo sapiens cDNA FLJ44754 fis, clone BRACE3030748, highly similar to Guanine nucleotide-binding protein G(i), alpha-2 subunit. BC012138 - Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, mRNA (cDNA clone MGC:20233 IMAGE:4562585), complete cds. AK055574 - Homo sapiens cDNA FLJ31012 fis, clone HLUNG2000217, highly similar to Guanine nucleotide-binding protein G(i), alpha-2 subunit. JD024596 - Sequence 5620 from Patent EP1572962. BC014627 - Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, mRNA (cDNA clone MGC:4489 IMAGE:2963802), complete cds. X04828 - Human mRNA for G(i) protein alpha-subunit (adenylate cyclase inhibiting GTP-binding protein). JD030348 - Sequence 11372 from Patent EP1572962. J03004 - Human guanine nucleotide-binding regulatory protein (G) alpha-inhibitory-subunit mRNA, complete cds. CR456783 - Homo sapiens full open reading frame cDNA clone RZPDo834G034D for gene GNAI2, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2; complete cds, incl. stopcodon. DQ892783 - Synthetic construct clone IMAGE:100005413; FLH189695.01X; RZPDo839A0375D guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2) gene, encodes complete protein. AB463704 - Synthetic construct DNA, clone: pF1KB6472, Homo sapiens GNAI2 gene for guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, without stop codon, in Flexi system. DQ895908 - Synthetic construct Homo sapiens clone IMAGE:100010368; FLH189691.01L; RZPDo839A0365D guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 (GNAI2) gene, encodes complete protein. KJ905760 - Synthetic construct Homo sapiens clone ccsbBroadEn_15430 GNAI2 gene, encodes complete protein. CU677075 - Synthetic construct Homo sapiens gateway clone IMAGE:100017082 5' read GNAI2 mRNA. KJ896898 - Synthetic construct Homo sapiens clone ccsbBroadEn_06292 GNAI2 gene, encodes complete protein. AF493906 - Homo sapiens guanine nucleotide binding protein alpha i2 (GNAI2) mRNA, complete cds. AY677118 - Homo sapiens Galphai2 protein mRNA, complete cds, alternatively spliced. AK304473 - Homo sapiens cDNA FLJ61561 complete cds, highly similar to Guanine nucleotide-binding protein G(i), alpha-2 subunit. AK302330 - Homo sapiens cDNA FLJ61190 complete cds, highly similar to Guanine nucleotide-binding protein G(i), alpha-2 subunit. AK131062 - Homo sapiens cDNA FLJ29028 fis, clone RCT00196, highly similar to Guanine nucleotide-binding protein G(i), alpha-2 subunit. JD024762 - Sequence 5786 from Patent EP1572962. JD033501 - Sequence 14525 from Patent EP1572962. JD254502 - Sequence 235526 from Patent EP1572962. JD549931 - Sequence 530955 from Patent EP1572962. JD549932 - Sequence 530956 from Patent EP1572962. JD549933 - Sequence 530957 from Patent EP1572962. JD193908 - Sequence 174932 from Patent EP1572962. JD291105 - Sequence 272129 from Patent EP1572962. JD541011 - Sequence 522035 from Patent EP1572962. JD472985 - Sequence 454009 from Patent EP1572962. JD445929 - Sequence 426953 from Patent EP1572962. JD077299 - Sequence 58323 from Patent EP1572962. JD191656 - Sequence 172680 from Patent EP1572962. JD191585 - Sequence 172609 from Patent EP1572962. JD399524 - Sequence 380548 from Patent EP1572962. JD124335 - Sequence 105359 from Patent EP1572962. JD049504 - Sequence 30528 from Patent EP1572962. JD310608 - Sequence 291632 from Patent EP1572962. JD143777 - Sequence 124801 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P04899 (Reactome details) participates in the following event(s):
R-HSA-8850560 G-protein alpha releases G-protein heterotrimer from PDCL R-HSA-392206 G alpha (i) inhibits adenylate cyclase R-HSA-392212 G alpha (i) auto-inactivates by hydrolysing GTP to GDP R-HSA-392202 Dissociation of the P2Y purinoceptor 12:Gi complex R-HSA-749454 The Ligand:GPCR:Gi complex dissociates R-HSA-751001 Inactive G alpha (i) reassociates with G beta:gamma R-HSA-170674 Dissociation of the Gi alpha:G olf complex R-HSA-167433 G-protein beta-gamma subunits rebind the alpha-GDP subunit R-HSA-112037 Inactivation of PLC beta R-HSA-167415 G-protein alpha subunit is inactivated R-HSA-111870 Activation of PLC beta-1/4 R-HSA-112271 The receptor:G-protein complex dissociates R-HSA-167429 The receptor:G-protein complex binds GTP R-HSA-400037 Gi,Go Heterotrimeric G-protein complex dissociates R-HSA-170671 G alpha-olf:GTP binds to Gi alpha1:GTP:adenylate cyclase complex R-HSA-170686 Adenylate cyclase increases the GTPase activity of Gi alpha R-HSA-392187 Activated P2Y purinoceptor 12 binds G-protein Gi R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi R-HSA-167408 The high affinity receptor complex binds to G-protein R-HSA-167419 The receptor:G-protein complex releases GDP R-HSA-400092 Alpha-2A,alpha-2C Adrenergic Receptors activate Gi.Go heterotrimeric G proteins R-HSA-392195 Gi activation by P2Y purinoceptor 12 R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi R-HSA-170666 Adenylate cyclase increases the GTPase activity of G alpha-olf R-HSA-111879 PIP2 hydrolysis R-HSA-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding R-HSA-170670 Adenylate cyclase inhibitory pathway R-HSA-418594 G alpha (i) signalling events R-HSA-392170 ADP signalling through P2Y purinoceptor 12 R-HSA-202040 G-protein activation R-HSA-112043 PLC beta mediated events R-HSA-390466 Chaperonin-mediated protein folding R-HSA-400042 Adrenaline,noradrenaline inhibits insulin secretion R-HSA-112040 G-protein mediated events R-HSA-997269 Inhibition of adenylate cyclase pathway R-HSA-388396 GPCR downstream signalling R-HSA-392518 Signal amplification R-HSA-111885 Opioid Signalling R-HSA-391251 Protein folding R-HSA-422356 Regulation of insulin secretion R-HSA-991365 Activation of GABAB receptors R-HSA-372790 Signaling by GPCR R-HSA-76002 Platelet activation, signaling and aggregation R-HSA-392499 Metabolism of proteins R-HSA-163685 Energy Metabolism R-HSA-977444 GABA B receptor activation R-HSA-162582 Signal Transduction R-HSA-109582 Hemostasis R-HSA-1430728 Metabolism R-HSA-977443 GABA receptor activation R-HSA-112314 Neurotransmitter receptors and postsynaptic signal transmission R-HSA-112315 Transmission across Chemical Synapses R-HSA-112316 Neuronal System
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
