Human Gene GPBAR1 (ENST00000519574.2_4) from GENCODE V47lift37
  Description: G protein-coupled bile acid receptor 1, transcript variant 3 (from RefSeq NM_170699.3)
Gencode Transcript: ENST00000519574.2_4
Gencode Gene: ENSG00000179921.15_7
Transcript (Including UTRs)
   Position: hg19 chr2:219,125,738-219,128,584 Size: 2,847 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr2:219,127,448-219,128,440 Size: 993 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:219,125,738-219,128,584)mRNA (may differ from genome)Protein (330 aa)
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UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GPBAR_HUMAN
DESCRIPTION: RecName: Full=G-protein coupled bile acid receptor 1; AltName: Full=G-protein coupled receptor GPCR19; Short=hGPCR19; AltName: Full=Membrane-type receptor for bile acids; Short=M-BAR; AltName: Full=hBG37; Short=BG37;
FUNCTION: Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: GPBAR1
Diseases sorted by gene-association score: sclerosing cholangitis (12), ocular albinism (8), cholangitis (8), functional diarrhea (7), obstructive jaundice (6), biliary tract disease (1), bile duct disease (1), cholangitis, primary sclerosing (1), leber congenital amaurosis (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.78 RPKM in Adipose - Visceral (Omentum)
Total median expression: 42.70 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -103.00247-0.417 Picture PostScript Text
3' UTR -33.10144-0.230 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q8TDU6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0038181 bile acid receptor activity
GO:0038182 G-protein coupled bile acid receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0038184 cell surface bile acid receptor signaling pathway
GO:2000810 regulation of bicellular tight junction assembly

Cellular Component:
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK122658 - Homo sapiens cDNA FLJ16092 fis, clone PLACE6002312, highly similar to G-protein coupled bile acid receptor 1.
AK122660 - Homo sapiens cDNA FLJ16095 fis, clone SPLEN2005869, highly similar to G-protein coupled bile acid receptor 1.
BC033625 - Homo sapiens G protein-coupled bile acid receptor 1, mRNA (cDNA clone MGC:40597 IMAGE:5221127), complete cds.
JD327312 - Sequence 308336 from Patent EP1572962.
JD108842 - Sequence 89866 from Patent EP1572962.
JD543711 - Sequence 524735 from Patent EP1572962.
JD325036 - Sequence 306060 from Patent EP1572962.
JD133807 - Sequence 114831 from Patent EP1572962.
JD372558 - Sequence 353582 from Patent EP1572962.
JD170373 - Sequence 151397 from Patent EP1572962.
AB464538 - Synthetic construct DNA, clone: pF1KB8994, Homo sapiens GPBAR1 gene for G protein-coupled bile acid receptor 1, without stop codon, in Flexi system.
DQ894522 - Synthetic construct Homo sapiens clone IMAGE:100008982; FLH171219.01L; RZPDo839G1299D G protein-coupled bile acid receptor 1 (GPBAR1) gene, encodes complete protein.
DQ891340 - Synthetic construct clone IMAGE:100003970; FLH171223.01X; RZPDo839G12100D G protein-coupled bile acid receptor 1 (GPBAR1) gene, encodes complete protein.
KJ895655 - Synthetic construct Homo sapiens clone ccsbBroadEn_05049 GPBAR1 gene, encodes complete protein.
AB086170 - Homo sapiens mRNA for membrane bile acid receptor, complete cds.
AB089307 - Homo sapiens TGR5 mRNA for G protein-coupled receptor, complete cds.
JD086299 - Sequence 67323 from Patent EP1572962.
JD104209 - Sequence 85233 from Patent EP1572962.
JD103666 - Sequence 84690 from Patent EP1572962.
JD389782 - Sequence 370806 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8TDU6 (Reactome details) participates in the following event(s):

R-HSA-444654 G-protein coupled bile acid receptor binds lithocholic acid
R-HSA-744886 The Ligand:GPCR:Gs complex dissociates
R-HSA-744887 Liganded Gs-activating GPCRs bind inactive heterotrimeric Gs
R-HSA-379044 Liganded Gs-activating GPCR acts as a GEF for Gs
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-418555 G alpha (s) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B3KV35, ENST00000519574.1, GPBAR_HUMAN, NM_170699, Q8TDU6, TGR5, uc323sll.1, uc323sll.2
UCSC ID: ENST00000519574.2_4
RefSeq Accession: NM_170699.3
Protein: Q8TDU6 (aka GPBAR_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.