Human Gene ADGRF5 (ENST00000265417.7_7) from GENCODE V47lift37
  Description: adhesion G protein-coupled receptor F5, transcript variant 1 (from RefSeq NM_015234.5)
Gencode Transcript: ENST00000265417.7_7
Gencode Gene: ENSG00000069122.19_10
Transcript (Including UTRs)
   Position: hg19 chr6:46,820,336-46,922,680 Size: 102,345 Total Exon Count: 21 Strand: -
Coding Region
   Position: hg19 chr6:46,821,729-46,874,499 Size: 52,771 Coding Exon Count: 20 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:46,820,336-46,922,680)mRNA (may differ from genome)Protein (1346 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGeneCardsMGIPubMed
ReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GP116_HUMAN
DESCRIPTION: RecName: Full=Probable G-protein coupled receptor 116; Flags: Precursor;
FUNCTION: May have a role in the regulation of acid-base balance (By similarity).
SUBUNIT: Exists as disulfide-linked dimers at the cell surface (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (Potential).
PTM: Proteolytically cleaved into 2 highly conserved sites: one in the SEA domain and the other in the stalk domain region preceding the first transmembrane. The later 2 subunits, the extracellular subunit and the seven-transmembrane subunit, remain tightly associated and non-covalently linked (By similarity).
SIMILARITY: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
SIMILARITY: Contains 1 GPS domain.
SIMILARITY: Contains 3 Ig-like (immunoglobulin-like) domains.
SIMILARITY: Contains 1 SEA domain.
SEQUENCE CAUTION: Sequence=BAA34478.2; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=CAB43394.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 61.76 RPKM in Lung
Total median expression: 516.57 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -62.20234-0.266 Picture PostScript Text
3' UTR -334.401393-0.240 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR017981 - GPCR_2-like
IPR008078 - GPCR_2_Ig-hepta_rcpt
IPR000832 - GPCR_2_secretin-like
IPR017983 - GPCR_2_secretin-like_CS
IPR000203 - GPS_dom
IPR007110 - Ig-like
IPR013783 - Ig-like_fold
IPR013098 - Ig_I-set
IPR003599 - Ig_sub
IPR003598 - Ig_sub2
IPR000082 - SEA

Pfam Domains:
PF00002 - 7 transmembrane receptor (Secretin family)
PF01390 - SEA domain
PF01825 - GPCR proteolysis site, GPS, motif
PF07679 - Immunoglobulin I-set domain
PF13927 - Immunoglobulin domain

SCOP Domains:
48726 - Immunoglobulin
82671 - SEA domain
81321 - Family A G protein-coupled receptor-like
57059 - omega toxin-like

ModBase Predicted Comparative 3D Structure on Q8IZF2
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004888 transmembrane signaling receptor activity
GO:0004930 G-protein coupled receptor activity

Biological Process:
GO:0003094 glomerular filtration
GO:0006112 energy reserve metabolic process
GO:0007165 signal transduction
GO:0007166 cell surface receptor signaling pathway
GO:0007186 G-protein coupled receptor signaling pathway
GO:0019216 regulation of lipid metabolic process
GO:0042593 glucose homeostasis
GO:0043031 negative regulation of macrophage activation
GO:0043129 surfactant homeostasis
GO:0045444 fat cell differentiation
GO:0048821 erythrocyte development
GO:0061626 pharyngeal arch artery morphogenesis
GO:0071073 positive regulation of phospholipid biosynthetic process

Cellular Component:
GO:0005886 plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0031410 cytoplasmic vesicle
GO:0045177 apical part of cell


-  Descriptions from all associated GenBank mRNAs
  BC066121 - Homo sapiens G protein-coupled receptor 116, mRNA (cDNA clone MGC:71511 IMAGE:5298732), complete cds.
BC036002 - Homo sapiens G protein-coupled receptor 116, mRNA (cDNA clone IMAGE:4723332), with apparent retained intron.
AB018301 - Homo sapiens KIAA0758 mRNA for KIAA0758 protein.
JD380135 - Sequence 361159 from Patent EP1572962.
JD159355 - Sequence 140379 from Patent EP1572962.
AK075121 - Homo sapiens cDNA FLJ90640 fis, clone PLACE1003852, moderately similar to G-protein coupled receptor 116.
AB031421 - Homo sapiens KPG_001 mRNA for Ig-Hepta homolog, complete cds.
AK289503 - Homo sapiens cDNA FLJ75412 complete cds, highly similar to Homo sapiens G protein-coupled receptor 116 (GPR116), mRNA.
AK296948 - Homo sapiens cDNA FLJ57030 complete cds, highly similar to Probable G-protein coupled receptor 116 precursor.
BC050087 - Homo sapiens G protein-coupled receptor 116, mRNA (cDNA clone IMAGE:5267719), partial cds.
AL832125 - Homo sapiens mRNA; cDNA DKFZp686D1010 (from clone DKFZp686D1010).
AK300380 - Homo sapiens cDNA FLJ57199 complete cds, highly similar to Probable G-protein coupled receptor 116 precursor.
AY498875 - Homo sapiens G protein coupled receptor 116 (GPR116) mRNA, complete cds.
AB383979 - Synthetic construct DNA, clone: pF1KSDA0758, Homo sapiens GPR116 gene for G-protein coupled receptor 116, complete cds, without stop codon, in Flexi system.
EU832495 - Synthetic construct Homo sapiens clone HAIB:100067524; DKFZo008F0229 G protein-coupled receptor 116 protein (GPR116) gene, encodes complete protein.
EU832576 - Synthetic construct Homo sapiens clone HAIB:100067605; DKFZo004F0230 G protein-coupled receptor 116 protein (GPR116) gene, encodes complete protein.
AM393514 - Synthetic construct Homo sapiens clone IMAGE:100002493 for hypothetical protein (GPR116 gene).
AY140958 - Homo sapiens G-protein coupled receptor GPR116 (GPR116) mRNA, complete cds.
AY255552 - Homo sapiens G protein-coupled receptor KIAA0758 protein mRNA, partial cds.
JD092942 - Sequence 73966 from Patent EP1572962.
AL050295 - Homo sapiens mRNA; cDNA DKFZp564O1923 (from clone DKFZp564O1923).
AK092043 - Homo sapiens cDNA FLJ34724 fis, clone MESAN2005957, moderately similar to Rattus norvegicus mRNA for seven transmembrane receptor.
AX747330 - Sequence 855 from Patent EP1308459.

-  Other Names for This Gene
  Alternate Gene Symbols: ADGRF5 , AGRF5_HUMAN, ENST00000265417.1, ENST00000265417.2, ENST00000265417.3, ENST00000265417.4, ENST00000265417.5, ENST00000265417.6, GPR116, KIAA0758, NM_015234, O94858, Q5TF06, Q6RGN2, Q86SP0, Q8IZF2, Q9Y3Z2, uc317ibf.1, uc317ibf.2
UCSC ID: ENST00000265417.7_7
RefSeq Accession: NM_015234.5
Protein: Q8IZF2 (aka GP116_HUMAN or G116_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
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