Human Gene GPR18 (ENST00000397470.5_8) from GENCODE V47lift37
  Description: G protein-coupled receptor 18, transcript variant 2 (from RefSeq NM_001098200.2)
Gencode Transcript: ENST00000397470.5_8
Gencode Gene: ENSG00000125245.14_12
Transcript (Including UTRs)
   Position: hg19 chr13:99,906,993-99,910,633 Size: 3,641 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr13:99,907,131-99,908,126 Size: 996 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr13:99,906,993-99,910,633)mRNA (may differ from genome)Protein (331 aa)
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-  Comments and Description Text from UniProtKB
  ID: GPR18_HUMAN
DESCRIPTION: RecName: Full=N-arachidonyl glycine receptor; Short=NAGly receptor; AltName: Full=G-protein coupled receptor 18;
FUNCTION: Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
SEQUENCE CAUTION: Sequence=AAG44671.1; Type=Frameshift; Positions=78, 81, 129;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.44 RPKM in Testis
Total median expression: 24.30 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -43.60260-0.168 Picture PostScript Text
3' UTR -5.80138-0.042 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR002286 - P2_purnocptor

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q14330
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity

Biological Process:
GO:0002300 CD8-positive, alpha-beta intraepithelial T cell differentiation
GO:0002305 CD8-positive, gamma-delta intraepithelial T cell differentiation
GO:0002689 negative regulation of leukocyte chemotaxis
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0030217 T cell differentiation
GO:0032720 negative regulation of tumor necrosis factor production
GO:0035025 positive regulation of Rho protein signal transduction
GO:0051482 positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030659 cytoplasmic vesicle membrane
GO:0031410 cytoplasmic vesicle


-  Descriptions from all associated GenBank mRNAs
  LF385022 - JP 2014500723-A/192525: Polycomb-Associated Non-Coding RNAs.
MA620599 - JP 2018138019-A/192525: Polycomb-Associated Non-Coding RNAs.
BC050646 - Homo sapiens G protein-coupled receptor 18, mRNA (cDNA clone MGC:60141 IMAGE:4341573), complete cds.
AF261135 - Homo sapiens GPR18-iso mRNA, complete cds.
AF339781 - Homo sapiens clone IMAGE:1837189, mRNA sequence.
BC066927 - Homo sapiens G protein-coupled receptor 18, mRNA (cDNA clone MGC:87213 IMAGE:4373724), complete cds.
BC008569 - Homo sapiens G protein-coupled receptor 18, mRNA (cDNA clone MGC:17339 IMAGE:4338810), complete cds.
JQ308176 - Homo sapiens G protein-coupled receptor 18 mRNA, complete cds.
KJ891281 - Synthetic construct Homo sapiens clone ccsbBroadEn_00675 GPR18 gene, encodes complete protein.
BT009907 - Homo sapiens G protein-coupled receptor 18 mRNA, complete cds.
AB463659 - Synthetic construct DNA, clone: pF1KB7138, Homo sapiens GPR18 gene for G protein-coupled receptor 18, without stop codon, in Flexi system.
JD354284 - Sequence 335308 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q14330 (Reactome details) participates in the following event(s):

R-HSA-517536 N-arachidonyl glycine receptor GPR18 binds N-arachidonyl glycine
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-418594 G alpha (i) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000397470.1, ENST00000397470.2, ENST00000397470.3, ENST00000397470.4, GPCRW, GPR18_HUMAN, NM_001098200, Q14330, Q6GTM3, Q96HI6, Q9H2L2, uc318zau.1, uc318zau.2
UCSC ID: ENST00000397470.5_8
RefSeq Accession: NM_001098200.2
Protein: Q14330 (aka GPR18_HUMAN or GP18_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.