Human Gene GPR183 (ENST00000376414.5_7) from GENCODE V47lift37
  Description: G protein-coupled receptor 183 (from RefSeq NM_004951.5)
Gencode Transcript: ENST00000376414.5_7
Gencode Gene: ENSG00000169508.7_9
Transcript (Including UTRs)
   Position: hg19 chr13:99,946,793-99,959,653 Size: 12,861 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr13:99,947,314-99,948,399 Size: 1,086 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr13:99,946,793-99,959,653)mRNA (may differ from genome)Protein (361 aa)
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HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GP183_HUMAN
DESCRIPTION: RecName: Full=G-protein coupled receptor 183; AltName: Full=Epstein-Barr virus-induced G-protein coupled receptor 2; Short=EBI2; Short=EBV-induced G-protein coupled receptor 2;
FUNCTION: Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Binding of 7- alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). Signals constitutively through G(i)-alpha, but not throughG(s)-alpha or G(q)-alpha. Signals constitutively also via MAPK1/3 (ERK1/2).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed abundantly in lymphoid tissues such as spleen and lymph node, and in B- and T-lymphocytes. Also highly expressed in lung, heart and gastrointestinal tract, and weakly expressed in the urogenital system and brain.
INDUCTION: By Epstein-Barr virus (EBV).
MISCELLANEOUS: GSK682753A (8-[(2E)-3-(4-chlorophenyl)prop-2- enoyl]-3-[(3,4-dichlorophenyl)methyl]-1-oxa-3,8- diazaspiro[4.5]decan-2-one), an inverse agonist, selectively inhibits the constitutive activity of GPR183 with high potency and efficacy (PubMed:21673108).
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CAUTION: It is uncertain whether Met-1 or Met-5 is the initiator.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 62.81 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 302.80 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -7.0078-0.090 Picture PostScript Text
3' UTR -95.00521-0.182 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR002286 - P2_purnocptor

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)
PF10328 - Serpentine type 7TM GPCR chemoreceptor Srx

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on P32249
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0008142 oxysterol binding

Biological Process:
GO:0002250 adaptive immune response
GO:0002312 B cell activation involved in immune response
GO:0002313 mature B cell differentiation involved in immune response
GO:0002376 immune system process
GO:0002407 dendritic cell chemotaxis
GO:0006955 immune response
GO:0006959 humoral immune response
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0010818 T cell chemotaxis
GO:0030316 osteoclast differentiation
GO:0030595 leukocyte chemotaxis
GO:0030890 positive regulation of B cell proliferation
GO:0036145 dendritic cell homeostasis
GO:0060326 cell chemotaxis
GO:0061470 T follicular helper cell differentiation
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:2000458 regulation of astrocyte chemotaxis

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  LF385022 - JP 2014500723-A/192525: Polycomb-Associated Non-Coding RNAs.
MA620599 - JP 2018138019-A/192525: Polycomb-Associated Non-Coding RNAs.
AK223390 - Homo sapiens mRNA for EBV-induced G protein-coupled receptor 2 variant, clone: FCC107B08.
BC020752 - Homo sapiens G protein-coupled receptor 183, mRNA (cDNA clone MGC:22599 IMAGE:4722289), complete cds.
L08177 - Human EBV induced G-protein coupled receptor (EBI2) mRNA, complete cds.
JD562466 - Sequence 543490 from Patent EP1572962.
JD358707 - Sequence 339731 from Patent EP1572962.
JD109940 - Sequence 90964 from Patent EP1572962.
AK292091 - Homo sapiens cDNA FLJ78219 complete cds, highly similar to Homo sapiens Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupled receptor) (EBI2), mRNA.
AK313443 - Homo sapiens cDNA, FLJ93982, highly similar to Homo sapiens Epstein-Barr virus induced gene 2 (lymphocyte-specificG protein-coupled receptor) (EBI2), mRNA.
KJ891079 - Synthetic construct Homo sapiens clone ccsbBroadEn_00473 GPR183 gene, encodes complete protein.
DQ891368 - Synthetic construct clone IMAGE:100003998; FLH176284.01X; RZPDo839B07122D Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupled receptor) (EBI2) gene, encodes complete protein.
DQ894548 - Synthetic construct Homo sapiens clone IMAGE:100009008; FLH176280.01L; RZPDo839B07121D Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupled receptor) (EBI2) gene, encodes complete protein.
AB464515 - Synthetic construct DNA, clone: pF1KB8810, Homo sapiens EBI2 gene for Epstein-Barr virus induced gene 2, without stop codon, in Flexi system.
CU676694 - Synthetic construct Homo sapiens gateway clone IMAGE:100022220 5' read EBI2 mRNA.
JD157707 - Sequence 138731 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P32249 (Reactome details) participates in the following event(s):

R-HSA-8851679 GPR183 binds 7alpha,25-dihydroxycholesterol
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-418594 G alpha (i) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B2R8N5, EBI2 , ENST00000376414.1, ENST00000376414.2, ENST00000376414.3, ENST00000376414.4, GP183_HUMAN, GPR183 , NM_004951, P32249, Q53F99, Q5JUH7, uc318mxt.1, uc318mxt.2
UCSC ID: ENST00000376414.5_7
RefSeq Accession: NM_004951.5
Protein: P32249 (aka GP183_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.