Human Gene GPR25 (ENST00000304244.5_5) from GENCODE V47lift37
  Description: G protein-coupled receptor 25 (from RefSeq NM_005298.4)
Gencode Transcript: ENST00000304244.5_5
Gencode Gene: ENSG00000170128.5_8
Transcript (Including UTRs)
   Position: hg19 chr1:200,842,109-200,843,306 Size: 1,198 Total Exon Count: 1 Strand: +
Coding Region
   Position: hg19 chr1:200,842,166-200,843,251 Size: 1,086 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:200,842,109-200,843,306)mRNA (may differ from genome)Protein (361 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GPR25_HUMAN
DESCRIPTION: RecName: Full=Probable G-protein coupled receptor 25;
FUNCTION: Orphan receptor.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.76 RPKM in Testis
Total median expression: 3.72 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -22.7057-0.398 Picture PostScript Text
3' UTR -19.8055-0.360 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on O00155
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0005515 protein binding

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  LF205366 - JP 2014500723-A/12869: Polycomb-Associated Non-Coding RNAs.
BC126386 - Homo sapiens G protein-coupled receptor 25, mRNA (cDNA clone MGC:161664 IMAGE:8992102), complete cds.
BC126388 - Homo sapiens G protein-coupled receptor 25, mRNA (cDNA clone MGC:161666 IMAGE:8992104), complete cds.
AK315339 - Homo sapiens cDNA, FLJ96381.
KJ896922 - Synthetic construct Homo sapiens clone ccsbBroadEn_06316 GPR25 gene, encodes complete protein.
JD404368 - Sequence 385392 from Patent EP1572962.
MA440943 - JP 2018138019-A/12869: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O00155 (Reactome details) participates in the following event(s):

R-HSA-744887 Liganded Gs-activating GPCRs bind inactive heterotrimeric Gs
R-HSA-744886 The Ligand:GPCR:Gs complex dissociates
R-HSA-379044 Liganded Gs-activating GPCR acts as a GEF for Gs
R-HSA-418555 G alpha (s) signalling events
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A0AVJ5, ENST00000304244.1, ENST00000304244.2, ENST00000304244.3, ENST00000304244.4, GPR25_HUMAN, NM_005298, O00155, uc317nju.1, uc317nju.2
UCSC ID: ENST00000304244.5_5
RefSeq Accession: NM_005298.4
Protein: O00155 (aka GPR25_HUMAN or GP25_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.