Human Gene GPR55 (ENST00000650999.1_6) from GENCODE V47lift37
  Description: G protein-coupled receptor 55 (from RefSeq NM_005683.4)
Gencode Transcript: ENST00000650999.1_6
Gencode Gene: ENSG00000135898.10_9
Transcript (Including UTRs)
   Position: hg19 chr2:231,772,043-231,789,957 Size: 17,915 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr2:231,774,718-231,775,677 Size: 960 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:231,772,043-231,789,957)mRNA (may differ from genome)Protein (319 aa)
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HGNCMGIOMIMPubMedReactomeUniProtKB
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-  Comments and Description Text from UniProtKB
  ID: GPR55_HUMAN
DESCRIPTION: RecName: Full=G-protein coupled receptor 55;
FUNCTION: May be involved in hyperalgesia associated with inflammatory and neuropathic pain (By similarity). Receptor for L- alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.
MISCELLANEOUS: The classification of this protein as a cannabinoid receptor remains a contentious issue due to conflicting pharmacological results.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
SEQUENCE CAUTION: Sequence=AAD22410.1; Type=Frameshift; Positions=132, 142;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -59.60209-0.285 Picture PostScript Text
3' UTR -1025.702675-0.383 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q9Y2T6
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0004949 cannabinoid receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007202 activation of phospholipase C activity
GO:0035025 positive regulation of Rho protein signal transduction
GO:0038171 cannabinoid signaling pathway
GO:0045453 bone resorption
GO:0045671 negative regulation of osteoclast differentiation
GO:0051482 positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
GO:0070374 positive regulation of ERK1 and ERK2 cascade

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  BC032694 - Homo sapiens G protein-coupled receptor 55, mRNA (cDNA clone MGC:45233 IMAGE:5228678), complete cds.
JD290125 - Sequence 271149 from Patent EP1572962.
JD192889 - Sequence 173913 from Patent EP1572962.
JD455216 - Sequence 436240 from Patent EP1572962.
JD342997 - Sequence 324021 from Patent EP1572962.
JD225630 - Sequence 206654 from Patent EP1572962.
JD085242 - Sequence 66266 from Patent EP1572962.
JD507123 - Sequence 488147 from Patent EP1572962.
JD510501 - Sequence 491525 from Patent EP1572962.
JD277190 - Sequence 258214 from Patent EP1572962.
JD181499 - Sequence 162523 from Patent EP1572962.
JD167077 - Sequence 148101 from Patent EP1572962.
JD123534 - Sequence 104558 from Patent EP1572962.
JD122965 - Sequence 103989 from Patent EP1572962.
JD535064 - Sequence 516088 from Patent EP1572962.
JD479214 - Sequence 460238 from Patent EP1572962.
JD399476 - Sequence 380500 from Patent EP1572962.
JD466439 - Sequence 447463 from Patent EP1572962.
JD256379 - Sequence 237403 from Patent EP1572962.
JD439098 - Sequence 420122 from Patent EP1572962.
JD103186 - Sequence 84210 from Patent EP1572962.
JD103185 - Sequence 84209 from Patent EP1572962.
JD496442 - Sequence 477466 from Patent EP1572962.
JD222490 - Sequence 203514 from Patent EP1572962.
JD048485 - Sequence 29509 from Patent EP1572962.
JD496852 - Sequence 477876 from Patent EP1572962.
JD331258 - Sequence 312282 from Patent EP1572962.
JD288766 - Sequence 269790 from Patent EP1572962.
JD362105 - Sequence 343129 from Patent EP1572962.
JD176888 - Sequence 157912 from Patent EP1572962.
JD146690 - Sequence 127714 from Patent EP1572962.
JD237531 - Sequence 218555 from Patent EP1572962.
JD410820 - Sequence 391844 from Patent EP1572962.
JD206967 - Sequence 187991 from Patent EP1572962.
JD273476 - Sequence 254500 from Patent EP1572962.
AK292223 - Homo sapiens cDNA FLJ75410 complete cds, highly similar to Homo sapiens G protein-coupled receptor 55, mRNA.
JD449228 - Sequence 430252 from Patent EP1572962.
JD183137 - Sequence 164161 from Patent EP1572962.
FJ348259 - Homo sapiens G protein-coupled receptor 55 (GPR55) mRNA, complete cds.
DQ890881 - Synthetic construct clone IMAGE:100003511; FLH166750.01X; RZPDo839C1088D G protein-coupled receptor 55 (GPR55) gene, encodes complete protein.
KJ892734 - Synthetic construct Homo sapiens clone ccsbBroadEn_02128 GPR55 gene, encodes complete protein.
DQ894035 - Synthetic construct Homo sapiens clone IMAGE:100008495; FLH166746.01L; RZPDo839C1087D G protein-coupled receptor 55 (GPR55) gene, encodes complete protein.
CR541776 - Homo sapiens full open reading frame cDNA clone RZPDo834C1230D for gene GPR55, G protein-coupled receptor 55; complete cds, without stopcodon.
AB464547 - Synthetic construct DNA, clone: pF1KB8791, Homo sapiens GPR55 gene for G protein-coupled receptor 55, without stop codon, in Flexi system.
CU689490 - Synthetic construct Homo sapiens gateway clone IMAGE:100016698 5' read GPR55 mRNA.
JD449552 - Sequence 430576 from Patent EP1572962.
JD305161 - Sequence 286185 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9Y2T6 (Reactome details) participates in the following event(s):

R-HSA-419426 Cannabinoid receptors can bind cannabinoids
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-418594 G alpha (i) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: GPR55_HUMAN, NM_005683, Q8N580, Q9Y2T6, uc328sir.1, uc328sir.2
UCSC ID: ENST00000650999.1_6
RefSeq Accession: NM_005683.4
Protein: Q9Y2T6 (aka GPR55_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.