Human Gene GPR65 (ENST00000267549.5_4) from GENCODE V47lift37
  Description: G protein-coupled receptor 65 (from RefSeq NM_003608.4)
Gencode Transcript: ENST00000267549.5_4
Gencode Gene: ENSG00000140030.6_7
Transcript (Including UTRs)
   Position: hg19 chr14:88,471,479-88,481,155 Size: 9,677 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr14:88,477,192-88,478,205 Size: 1,014 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:88,471,479-88,481,155)mRNA (may differ from genome)Protein (337 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PSYR_HUMAN
DESCRIPTION: RecName: Full=Psychosine receptor; AltName: Full=G-protein coupled receptor 65; AltName: Full=T-cell death-associated gene 8 protein;
FUNCTION: Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a role in activation- induced cell death or differentiation of T-cells.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Predominantly expressed in thymus, spleen, lymph nodes, small intestine, lung, placenta and peripheral blood leukocytes.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: GPR65
Diseases sorted by gene-association score: rectal prolapse (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.59 RPKM in Spleen
Total median expression: 70.68 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -101.40547-0.185 Picture PostScript Text
3' UTR -845.702950-0.287 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR005464 - Psych_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q8IYL9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity

Biological Process:
GO:0006915 apoptotic process
GO:0006955 immune response
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007189 adenylate cyclase-activating G-protein coupled receptor signaling pathway
GO:0007275 multicellular organism development
GO:0010447 response to acidic pH
GO:0031532 actin cytoskeleton reorganization
GO:0035025 positive regulation of Rho protein signal transduction
GO:0051482 positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
GO:0051496 positive regulation of stress fiber assembly
GO:0090630 activation of GTPase activity

Cellular Component:
GO:0005622 intracellular
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  LQ740459 - Sequence 13 from Patent WO2018083189.
AK313797 - Homo sapiens cDNA, FLJ94412, highly similar to Homo sapiens G protein-coupled receptor 65 (GPR65), mRNA.
BC035633 - Homo sapiens G protein-coupled receptor 65, mRNA (cDNA clone MGC:45304 IMAGE:5218256), complete cds.
BC071715 - Homo sapiens G protein-coupled receptor 65, mRNA (cDNA clone MGC:88010 IMAGE:5180030), complete cds.
DL491772 - Novel nucleic acids.
JD353815 - Sequence 334839 from Patent EP1572962.
JD496255 - Sequence 477279 from Patent EP1572962.
JD516434 - Sequence 497458 from Patent EP1572962.
CU690142 - Synthetic construct Homo sapiens gateway clone IMAGE:100016734 5' read GPR65 mRNA.
DQ893989 - Synthetic construct Homo sapiens clone IMAGE:100008449; FLH166318.01L; RZPDo839F0685D G protein-coupled receptor 65 (GPR65) gene, encodes complete protein.
KJ897850 - Synthetic construct Homo sapiens clone ccsbBroadEn_07244 GPR65 gene, encodes complete protein.
EU883574 - Homo sapiens orphan GPCR 65 (GPR65) mRNA, complete cds.
DQ890834 - Synthetic construct clone IMAGE:100003464; FLH166322.01X; RZPDo839F0686D G protein-coupled receptor 65 (GPR65) gene, encodes complete protein.
JD380890 - Sequence 361914 from Patent EP1572962.
JD564020 - Sequence 545044 from Patent EP1572962.
JD263308 - Sequence 244332 from Patent EP1572962.
JD433975 - Sequence 414999 from Patent EP1572962.
JD312492 - Sequence 293516 from Patent EP1572962.
JD528271 - Sequence 509295 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8IYL9 (Reactome details) participates in the following event(s):

R-HSA-444731 GPR4, GPR65, GPR132 and OGR1 are pH sensing receptors
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-416476 G alpha (q) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000267549.1, ENST00000267549.2, ENST00000267549.3, ENST00000267549.4, NM_003608, O75819, PSYR_HUMAN, Q8IYL9, TDAG8, uc317iky.1, uc317iky.2
UCSC ID: ENST00000267549.5_4
RefSeq Accession: NM_003608.4
Protein: Q8IYL9 (aka PSYR_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.