Human Gene GPR68 (ENST00000650645.1_6) from GENCODE V47lift37
  Description: G protein-coupled receptor 68, transcript variant 1 (from RefSeq NM_001177676.2)
Gencode Transcript: ENST00000650645.1_6
Gencode Gene: ENSG00000119714.11_11
Transcript (Including UTRs)
   Position: hg19 chr14:91,698,876-91,711,048 Size: 12,173 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr14:91,700,297-91,701,394 Size: 1,098 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:91,698,876-91,711,048)mRNA (may differ from genome)Protein (365 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: OGR1_HUMAN
DESCRIPTION: RecName: Full=Ovarian cancer G-protein coupled receptor 1; Short=OGR-1; AltName: Full=G-protein coupled receptor 68; AltName: Full=GPR12A; AltName: Full=Sphingosylphosphorylcholine receptor;
FUNCTION: Proton-sensing receptor involved in pH homeostasis. May represents an osteoblastic pH sensor regulating cell-mediated responses to acidosis in bone. Mediates its action by association with G proteins that stimulates inositol phosphate (IP) production or Ca(2+) mobilization. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Function also as a metastasis suppressor gene in prostate cancer (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Found at low level in a wide range of tissues, but significantly expressed in lung, kidney, bone and nervous system.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CAUTION: Was originally (PubMed:10806476) thought to be a receptor for sphingosylphosphorylcholine (SPC). However, this work has been retracted (PubMed:16508674).
SEQUENCE CAUTION: Sequence=AAH96071.1; Type=Erroneous initiation; Sequence=AAH96072.1; Type=Erroneous initiation; Sequence=AAH96073.1; Type=Erroneous initiation; Sequence=AAH96074.1; Type=Erroneous initiation; Sequence=AAH98567.1; Type=Erroneous initiation; Sequence=AAT38917.1; Type=Erroneous initiation; Sequence=ACG60649.1; Type=Erroneous initiation; Sequence=EAW81447.1; Type=Erroneous initiation;
WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/GPR68ID40745ch14q32.html";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: GPR68
Diseases sorted by gene-association score: amelogenesis imperfecta, hypomaturation type, iia6* (1200), amelogenesis imperfecta* (453), amelogenesis imperfecta hypomaturation type* (219), rectal prolapse (7), cerebellar medulloblastoma (6), cerebellum cancer (6)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -216.80537-0.404 Picture PostScript Text
3' UTR -597.201421-0.420 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam
IPR005389 - OGR1_rcpt

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q15743
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity

Biological Process:
GO:0006954 inflammatory response
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0032024 positive regulation of insulin secretion
GO:0035774 positive regulation of insulin secretion involved in cellular response to glucose stimulus
GO:0045656 negative regulation of monocyte differentiation
GO:0071467 cellular response to pH
GO:2001206 positive regulation of osteoclast development

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  HQ447492 - Synthetic construct Homo sapiens clone IMAGE:100070825; CCSB013947_01 G protein-coupled receptor 68 (GPR68) gene, encodes complete protein.
BC098567 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone MGC:111379 IMAGE:30925589), complete cds.
JD278916 - Sequence 259940 from Patent EP1572962.
JD111950 - Sequence 92974 from Patent EP1572962.
U35398 - Human G protein-coupled receptor mRNA, complete cds.
BC096071 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone MGC:116706 IMAGE:40000421), complete cds.
BC096072 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone MGC:116707 IMAGE:40000422), complete cds.
BC096073 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone MGC:116708 IMAGE:40000423), complete cds.
BC096074 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone MGC:116709 IMAGE:40000425), complete cds.
KJ892470 - Synthetic construct Homo sapiens clone ccsbBroadEn_01864 GPR68 gene, encodes complete protein.
KR711746 - Synthetic construct Homo sapiens clone CCSBHm_00030748 GPR68 (GPR68) mRNA, encodes complete protein.
BC069592 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone MGC:97122 IMAGE:7262355), complete cds.
AY615372 - Homo sapiens ovarian cancer G protein-coupled receptor 1 mRNA, complete cds.
EU883575 - Homo sapiens orphan GPCR 68 (GPR68) mRNA, complete cds.
BC067470 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone IMAGE:6971805), partial cds.
BC067471 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone IMAGE:6971808), partial cds.
BC067472 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone IMAGE:6971809), partial cds.
AB463660 - Synthetic construct DNA, clone: pF1KB7162, Homo sapiens GPR68 gene for G protein-coupled receptor 68, without stop codon, in Flexi system.
KJ901860 - Synthetic construct Homo sapiens clone ccsbBroadEn_11254 GPR68 gene, encodes complete protein.
BC128536 - Homo sapiens G protein-coupled receptor 68, mRNA (cDNA clone MGC:156983 IMAGE:40120393), complete cds.
CU686918 - Synthetic construct Homo sapiens gateway clone IMAGE:100022647 5' read GPR68 mRNA.
JD069133 - Sequence 50157 from Patent EP1572962.
JD053978 - Sequence 35002 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q15743 (Reactome details) participates in the following event(s):

R-HSA-444731 GPR4, GPR65, GPR132 and OGR1 are pH sensing receptors
R-HSA-749452 The Ligand:GPCR:Gq complex dissociates
R-HSA-749448 Liganded Gq-activating GPCRs bind inactive heterotrimeric Gq
R-HSA-379048 Liganded Gq/11-activating GPCRs act as GEFs for Gq/11
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-416476 G alpha (q) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: NM_001177676, OGR1, OGR1_HUMAN, Q13334, Q15743, Q4VBB4, Q6IX34, uc328rwf.1, uc328rwf.2
UCSC ID: ENST00000650645.1_6
RefSeq Accession: NM_001177676.2
Protein: Q15743 (aka OGR1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.