Human Gene GPR84 (ENST00000267015.4_7) from GENCODE V47lift37
  Description: G protein-coupled receptor 84 (from RefSeq NM_020370.3)
Gencode Transcript: ENST00000267015.4_7
Gencode Gene: ENSG00000139572.4_9
Transcript (Including UTRs)
   Position: hg19 chr12:54,756,229-54,758,270 Size: 2,042 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr12:54,756,445-54,757,635 Size: 1,191 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:54,756,229-54,758,270)mRNA (may differ from genome)Protein (396 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GPR84_HUMAN
DESCRIPTION: RecName: Full=G-protein coupled receptor 84; AltName: Full=Inflammation-related G-protein coupled receptor EX33;
FUNCTION: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes.
INDUCTION: By bacterial lipopolysaccharides (LPS) in the monocytic leukaemia cell line THP-1.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 7.11 RPKM in Whole Blood
Total median expression: 22.13 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -25.10102-0.246 Picture PostScript Text
3' UTR -45.70216-0.212 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)
PF10328 - Serpentine type 7TM GPCR chemoreceptor Srx

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

ModBase Predicted Comparative 3D Structure on Q9NQS5
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0004966 galanin receptor activity

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007188 adenylate cyclase-modulating G-protein coupled receptor signaling pathway
GO:0007200 phospholipase C-activating G-protein coupled receptor signaling pathway
GO:0007218 neuropeptide signaling pathway
GO:0008150 biological_process
GO:0043312 neutrophil degranulation

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0035579 specific granule membrane
GO:0043235 receptor complex
GO:0070821 tertiary granule membrane


-  Descriptions from all associated GenBank mRNAs
  AF237762 - Homo sapiens orphan G protein-coupled receptor 84 (GPR84) mRNA, complete cds.
BC020614 - Homo sapiens G protein-coupled receptor 84, mRNA (cDNA clone MGC:22224 IMAGE:4279185), complete cds.
LP895870 - Sequence 734 from Patent EP3253886.
AF282693 - Homo sapiens inflammation-related G protein-coupled receptor EX33 (EX33) mRNA, complete cds.
AK290394 - Homo sapiens cDNA FLJ75402 complete cds, highly similar to Homo sapiens G protein-coupled receptor 84, mRNA.
JD387598 - Sequence 368622 from Patent EP1572962.
JD039449 - Sequence 20473 from Patent EP1572962.
JD091154 - Sequence 72178 from Patent EP1572962.
FJ348261 - Homo sapiens G protein-coupled receptor 84 (GPR84) mRNA, complete cds.
DQ893217 - Synthetic construct clone IMAGE:100005847; FLH195330.01X; RZPDo839B03152D G protein-coupled receptor 84 (GPR84) gene, encodes complete protein.
KJ894000 - Synthetic construct Homo sapiens clone ccsbBroadEn_03394 GPR84 gene, encodes complete protein.
DQ896546 - Synthetic construct Homo sapiens clone IMAGE:100011006; FLH195326.01L; RZPDo839B03151D G protein-coupled receptor 84 (GPR84) gene, encodes complete protein.
AB464549 - Synthetic construct DNA, clone: pF1KB8822, Homo sapiens GPR84 gene for G protein-coupled receptor 84, without stop codon, in Flexi system.
CU676578 - Synthetic construct Homo sapiens gateway clone IMAGE:100022262 5' read GPR84 mRNA.
MP067525 - Sequence 29 from Patent WO2018232288.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9NQS5 (Reactome details) participates in the following event(s):

R-HSA-6799350 Exocytosis of specific granule membrane proteins
R-HSA-6798747 Exocytosis of tertiary granule membrane proteins
R-HSA-744887 Liganded Gs-activating GPCRs bind inactive heterotrimeric Gs
R-HSA-744886 The Ligand:GPCR:Gs complex dissociates
R-HSA-379044 Liganded Gs-activating GPCR acts as a GEF for Gs
R-HSA-6798695 Neutrophil degranulation
R-HSA-168249 Innate Immune System
R-HSA-418555 G alpha (s) signalling events
R-HSA-168256 Immune System
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B6V9G7, ENST00000267015.1, ENST00000267015.2, ENST00000267015.3, EX33, GPR84_HUMAN, NM_020370, Q9NQS5, uc317ijb.1, uc317ijb.2
UCSC ID: ENST00000267015.4_7
RefSeq Accession: NM_020370.3
Protein: Q9NQS5 (aka GPR84_HUMAN)

-  Gene Model Information
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.