Human Gene GPX4 (ENST00000354171.13_10) from GENCODE V47lift37
  Description: glutathione peroxidase 4, transcript variant 1 (from RefSeq NM_002085.5)
Gencode Transcript: ENST00000354171.13_10
Gencode Gene: ENSG00000167468.20_16
Transcript (Including UTRs)
   Position: hg19 chr19:1,103,993-1,106,778 Size: 2,786 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr19:1,104,043-1,106,571 Size: 2,529 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:1,103,993-1,106,778)mRNA (may differ from genome)Protein (197 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GPX4_HUMAN
DESCRIPTION: RecName: Full=Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; Short=PHGPx; EC=1.11.1.12; AltName: Full=Glutathione peroxidase 4; Short=GPx-4; Short=GSHPx-4; Flags: Precursor;
FUNCTION: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity).
CATALYTIC ACTIVITY: 2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H(2)O.
SUBUNIT: Monomer. Has a tendency to form higher mass oligomers.
SUBCELLULAR LOCATION: Isoform Mitochondrial: Mitochondrion.
SUBCELLULAR LOCATION: Isoform Cytoplasmic: Cytoplasm.
TISSUE SPECIFICITY: Present primarily in testis.
SIMILARITY: Belongs to the glutathione peroxidase family.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/gpx4/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: GPX4
Diseases sorted by gene-association score: spondylometaphyseal dysplasia, sedaghatian type* (1369), shoulder impingement syndrome (11), neurotic disorder (9), miller-dieker lissencephaly syndrome (8), brachyolmia (6)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 743.15 RPKM in Testis
Total median expression: 6018.34 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -22.4050-0.448 Picture PostScript Text
3' UTR -89.50207-0.432 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000889 - Glutathione_peroxidase
IPR012336 - Thioredoxin-like_fold

Pfam Domains:
PF00255 - Glutathione peroxidase

SCOP Domains:
52833 - Thioredoxin-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2GS3 - X-ray MuPIT 2OBI - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P36969
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004601 peroxidase activity
GO:0004602 glutathione peroxidase activity
GO:0005515 protein binding
GO:0016491 oxidoreductase activity
GO:0042802 identical protein binding
GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity

Biological Process:
GO:0006644 phospholipid metabolic process
GO:0006979 response to oxidative stress
GO:0007275 multicellular organism development
GO:0007283 spermatogenesis
GO:0019372 lipoxygenase pathway
GO:0042759 long-chain fatty acid biosynthetic process
GO:0051258 protein polymerization
GO:0055114 oxidation-reduction process
GO:0098869 cellular oxidant detoxification

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0032991 macromolecular complex
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  BC039849 - Homo sapiens glutathione peroxidase 4 (phospholipid hydroperoxidase), mRNA (cDNA clone MGC:48873 IMAGE:5744771), complete cds.
X71973 - H.sapiens GPx-4 mRNA for phospholipid hydroperoxide glutathione peroxidase.
BC022071 - Homo sapiens glutathione peroxidase 4 (phospholipid hydroperoxidase), mRNA (cDNA clone MGC:33153 IMAGE:4821595), complete cds.
BC011836 - Homo sapiens glutathione peroxidase 4 (phospholipid hydroperoxidase), mRNA (cDNA clone MGC:20094 IMAGE:3945177), complete cds.
GQ891508 - Homo sapiens clone HEL-S-229 epididymis secretory sperm binding protein mRNA, complete cds.
BC032695 - Homo sapiens glutathione peroxidase 4 (phospholipid hydroperoxidase), mRNA (cDNA clone MGC:45347 IMAGE:5227887), complete cds.
AK225859 - Homo sapiens mRNA for Phospholipid hydroperoxide glutathione peroxidase, mitochondrial precursor variant, clone: FCC125B05.
BC058329 - Homo sapiens glutathione peroxidase 4 (phospholipid hydroperoxidase), mRNA (cDNA clone IMAGE:6527068).
BC021567 - Homo sapiens glutathione peroxidase 4 (phospholipid hydroperoxidase), mRNA (cDNA clone MGC:31923 IMAGE:4581069), complete cds.
BC046163 - Homo sapiens glutathione peroxidase 4 (phospholipid hydroperoxidase), mRNA (cDNA clone MGC:57716 IMAGE:5742889), complete cds.
CU692482 - Synthetic construct Homo sapiens gateway clone IMAGE:100020798 5' read GPX4 mRNA.
KJ891293 - Synthetic construct Homo sapiens clone ccsbBroadEn_00687 GPX4-like gene, encodes complete protein.
BC010157 - Homo sapiens glutathione peroxidase 4 (phospholipid hydroperoxidase), mRNA (cDNA clone IMAGE:3617438), complete cds.
KJ901465 - Synthetic construct Homo sapiens clone ccsbBroadEn_10859 GPX4 gene, encodes complete protein.
JD391660 - Sequence 372684 from Patent EP1572962.
JD213915 - Sequence 194939 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-4081 - glutathione-peroxide redox reactions

Reactome (by CSHL, EBI, and GO)

Protein P36969 (Reactome details) participates in the following event(s):

R-HSA-2161946 5S-HpETE is reduced to 5S-HETE by GPX1/2/4
R-HSA-2161959 12R-HpETE is reduced to 12R-HETE by GPX1/2/4
R-HSA-2161999 12S-HpETE is reduced to 12S-HETE by GPX1/2/4
R-HSA-2161791 15S-HpETE is reduced to 15S-HETE by GPX1/2/4
R-HSA-2142688 Synthesis of 5-eicosatetraenoic acids
R-HSA-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-HSA-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-HSA-2142753 Arachidonic acid metabolism
R-HSA-8978868 Fatty acid metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism
R-HSA-9018868 GPX4-2 reduces 18(S)-HpEPE to 18(S)-HEPE
R-HSA-9018895 GPX4-2 reduces 18(R)-HpEPE to 18(R)-HEPE
R-HSA-9020271 GPX4-2 reduces 17(R)-Hp-DHA to 17(R)-HDHA
R-HSA-9020273 GPX4-2 reduces 17(S)-Hp-DHA to 17(S)-HDHA
R-HSA-9018896 Biosynthesis of E-series 18(S)-resolvins
R-HSA-9023661 Biosynthesis of E-series 18(R)-resolvins
R-HSA-9020265 Biosynthesis of aspirin-triggered D-series resolvins
R-HSA-9018676 Biosynthesis of D-series resolvins
R-HSA-9018679 Biosynthesis of EPA-derived SPMs
R-HSA-9018677 Biosynthesis of DHA-derived SPMs
R-HSA-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
R-HSA-8978868 Fatty acid metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000354171.1, ENST00000354171.10, ENST00000354171.11, ENST00000354171.12, ENST00000354171.2, ENST00000354171.3, ENST00000354171.4, ENST00000354171.5, ENST00000354171.6, ENST00000354171.7, ENST00000354171.8, ENST00000354171.9, GPX4 , GPX4_HUMAN, NM_002085, O43381, P36969, Q6PJ59, Q9UPK2, uc317ymi.1, uc317ymi.2
UCSC ID: ENST00000354171.13_10
RefSeq Accession: NM_002085.5
Protein: P36969 (aka GPX4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.