Human Gene GSTA1 (ENST00000334575.6_7) from GENCODE V47lift37
  Description: glutathione S-transferase alpha 1, transcript variant 1 (from RefSeq NM_145740.5)
Gencode Transcript: ENST00000334575.6_7
Gencode Gene: ENSG00000243955.6_9
Transcript (Including UTRs)
   Position: hg19 chr6:52,656,169-52,668,614 Size: 12,446 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr6:52,656,656-52,664,065 Size: 7,410 Coding Exon Count: 6 

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Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:52,656,169-52,668,614)mRNA (may differ from genome)Protein (222 aa)
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WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GSTA1_HUMAN
DESCRIPTION: RecName: Full=Glutathione S-transferase A1; EC=2.5.1.18; AltName: Full=GST HA subunit 1; AltName: Full=GST class-alpha member 1; AltName: Full=GST-epsilon; AltName: Full=GSTA1-1; AltName: Full=GTH1;
FUNCTION: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
CATALYTIC ACTIVITY: RX + glutathione = HX + R-S-glutathione.
SUBUNIT: Homodimer or heterodimer of GSTA1 and GSTA2.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Liver.
DOMAIN: The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
SIMILARITY: Belongs to the GST superfamily. Alpha family.
SIMILARITY: Contains 1 GST C-terminal domain.
SIMILARITY: Contains 1 GST N-terminal domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: GSTA1
Diseases sorted by gene-association score: ovarian endodermal sinus tumor (9), ovarian primitive germ cell tumor (9), dental fluorosis (7), urinary bladder cancer (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 581.96 RPKM in Adrenal Gland
Total median expression: 1385.62 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -11.5062-0.185 Picture PostScript Text
3' UTR -98.20487-0.202 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR010987 - Glutathione-S-Trfase_C-like
IPR004045 - Glutathione_S-Trfase_N
IPR017933 - Glutathione_S_Trfase/Cl_chnl_C
IPR003080 - GST_alpha
IPR004046 - GST_C
IPR012336 - Thioredoxin-like_fold

Pfam Domains:
PF00043 - Glutathione S-transferase, C-terminal domain
PF02798 - Glutathione S-transferase, N-terminal domain
PF14497 - Glutathione S-transferase, C-terminal domain

SCOP Domains:
47616 - GST C-terminal domain-like
52833 - Thioredoxin-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1GSD - X-ray MuPIT 1GSE - X-ray MuPIT 1GSF - X-ray MuPIT 1GUH - X-ray MuPIT 1K3L - X-ray MuPIT 1K3O - X-ray MuPIT 1K3Y - X-ray MuPIT 1LBK - X-ray MuPIT 1PKW - X-ray MuPIT 1PKZ - X-ray MuPIT 1PL1 - X-ray MuPIT 1PL2 - X-ray MuPIT 1USB - X-ray MuPIT 1XWG - X-ray MuPIT 1YDK - X-ray MuPIT 2R3X - X-ray MuPIT 2R6K - X-ray MuPIT 3I69 - X-ray MuPIT 3I6A - X-ray MuPIT 3IK9 - X-ray MuPIT 3KTL - X-ray MuPIT 3L0H - X-ray MuPIT 3Q74 - X-ray MuPIT 3U6V - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P08263
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
 Gene Details    
 Gene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004364 glutathione transferase activity
GO:0004602 glutathione peroxidase activity
GO:0016740 transferase activity

Biological Process:
GO:0006749 glutathione metabolic process
GO:0008152 metabolic process
GO:0030855 epithelial cell differentiation
GO:0043651 linoleic acid metabolic process
GO:0098869 cellular oxidant detoxification
GO:1901687 glutathione derivative biosynthetic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  BC053578 - Homo sapiens glutathione S-transferase alpha 1, mRNA (cDNA clone MGC:61426 IMAGE:5167338), complete cds.
S49975 - glutathione transferase A1-1 [human, HepG2 hepatoma cells, mRNA, 790 nt].
BC110891 - Homo sapiens glutathione S-transferase alpha 1, mRNA (cDNA clone MGC:131939 IMAGE:5171697), complete cds.
M21758 - Human glutathione S-transferase (GST) Ha subunit 1 mRNA, complete cds.
M15872 - Human glutathione S-transferase 2 (GST) mRNA, complete cds.
M14777 - Human glutathione S-transferase (GST) a-subunit mRNA, complete cds.
M25627 - Human liver glutathione S-transferase subunit 1 mRNA, complete cds.
AK310324 - Homo sapiens cDNA, FLJ17366.
HM005393 - Homo sapiens clone HTL-T-80 testicular tissue protein Li 80 mRNA, complete cds.
AY532928 - Homo sapiens chromosome 6 glutathione S-transferase A1 mRNA, complete cds.
CU690990 - Synthetic construct Homo sapiens gateway clone IMAGE:100021634 5' read GSTA1 mRNA.
HQ448093 - Synthetic construct Homo sapiens clone IMAGE:100071473; CCSB011859_01 glutathione S-transferase A1 (GSTA1) gene, encodes complete protein.
KJ891307 - Synthetic construct Homo sapiens clone ccsbBroadEn_00701 GSTA1 gene, encodes complete protein.
CR407656 - Homo sapiens full open reading frame cDNA clone RZPDo834H082D for gene GSTA1, glutathione S-transferase A1 complete cds, without stopcodon.
AK293371 - Homo sapiens cDNA FLJ55406 complete cds, highly similar to Glutathione S-transferase A1 (EC 2.5.1.18).
DQ574559 - Homo sapiens piRNA piR-42671, complete sequence.
AL096729 - Homo sapiens mRNA; cDNA DKFZp434D044 (from clone DKFZp434D044).
JD072785 - Sequence 53809 from Patent EP1572962.
JD072784 - Sequence 53808 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-4061 - glutathione-mediated detoxification I
PWY-7112 - 4-hydroxy-2-nonenal detoxification

Reactome (by CSHL, EBI, and GO)

Protein P08263 (Reactome details) participates in the following event(s):

R-HSA-176054 GST dimers conjugate GSH with cytosolic substrates
R-HSA-156590 Glutathione conjugation
R-HSA-156580 Phase II - Conjugation of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000334575.1, ENST00000334575.2, ENST00000334575.3, ENST00000334575.4, ENST00000334575.5, GSTA1_HUMAN, NM_145740, P08263, Q14750, Q5GHF8, Q5SZC1, uc317uag.1, uc317uag.2
UCSC ID: ENST00000334575.6_7
RefSeq Accession: NM_145740.5
Protein: P08263 (aka GSTA1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.