Human Gene HTRA4 (ENST00000302495.5_4) from GENCODE V47lift37
  Description: HtrA serine peptidase 4 (from RefSeq NM_153692.4)
Gencode Transcript: ENST00000302495.5_4
Gencode Gene: ENSG00000169495.5_7
Transcript (Including UTRs)
   Position: hg19 chr8:38,831,747-38,846,182 Size: 14,436 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg19 chr8:38,831,783-38,845,617 Size: 13,835 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:38,831,747-38,846,182)mRNA (may differ from genome)Protein (476 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: HTRA4_HUMAN
DESCRIPTION: RecName: Full=Serine protease HTRA4; EC=3.4.21.-; AltName: Full=High-temperature requirement factor A4; Flags: Precursor;
FUNCTION: Serine protease (Probable).
SUBCELLULAR LOCATION: Secreted (By similarity).
SIMILARITY: Belongs to the peptidase S1B family.
SIMILARITY: Contains 1 IGFBP N-terminal domain.
SIMILARITY: Contains 1 Kazal-like domain.
SIMILARITY: Contains 1 PDZ (DHR) domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.77 RPKM in Lung
Total median expression: 5.57 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -4.1036-0.114 Picture PostScript Text
3' UTR -135.60565-0.240 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000867 - IGFBP-like
IPR011497 - Kazal-type_dom
IPR001478 - PDZ
IPR009003 - Pept_cys/ser_Trypsin-like
IPR001940 - Peptidase_S1C
IPR002350 - Prot_inh_Kazal

Pfam Domains:
PF00089 - Trypsin
PF00219 - Insulin-like growth factor binding protein
PF00595 - PDZ domain
PF07648 - Kazal-type serine protease inhibitor domain
PF13180 - PDZ domain
PF13365 - Trypsin-like peptidase domain
PF17820 - PDZ domain

SCOP Domains:
50156 - PDZ domain-like
50494 - Trypsin-like serine proteases
57184 - Growth factor receptor domain
100895 - Kazal-type serine protease inhibitors

ModBase Predicted Comparative 3D Structure on P83105
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004175 endopeptidase activity
GO:0004252 serine-type endopeptidase activity
GO:0005520 insulin-like growth factor binding
GO:0008233 peptidase activity
GO:0008236 serine-type peptidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0001558 regulation of cell growth
GO:0006508 proteolysis
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway

Cellular Component:
GO:0005576 extracellular region


-  Descriptions from all associated GenBank mRNAs
  LF208056 - JP 2014500723-A/15559: Polycomb-Associated Non-Coding RNAs.
LF212191 - JP 2014500723-A/19694: Polycomb-Associated Non-Coding RNAs.
AK075205 - Homo sapiens cDNA FLJ90724 fis, clone PLACE1009279, highly similar to Probable serine protease HTRA4 precursor (EC3.4.21.-).
BC057765 - Homo sapiens HtrA serine peptidase 4, mRNA (cDNA clone MGC:71517 IMAGE:30348811), complete cds.
JD382652 - Sequence 363676 from Patent EP1572962.
AK300197 - Homo sapiens cDNA FLJ51303 complete cds, highly similar to Probable serine protease HTRA4 precursor (EC 3.4.21.-).
KJ900432 - Synthetic construct Homo sapiens clone ccsbBroadEn_09826 HTRA4 gene, encodes complete protein.
AY188774 - Homo sapiens unknown mRNA, partial sequence.
MA443633 - JP 2018138019-A/15559: Polycomb-Associated Non-Coding RNAs.
MA447768 - JP 2018138019-A/19694: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000302495.1, ENST00000302495.2, ENST00000302495.3, ENST00000302495.4, HTRA4_HUMAN, NM_153692, P83105, Q542Z4, Q6PF13, uc317nbc.1, uc317nbc.2
UCSC ID: ENST00000302495.5_4
RefSeq Accession: NM_153692.4
Protein: P83105 (aka HTRA4_HUMAN or HRA4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.