Human Gene IDH3G (ENST00000217901.10_7) from GENCODE V47lift37
  Description: isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma, transcript variant 1 (from RefSeq NM_004135.4)
Gencode Transcript: ENST00000217901.10_7
Gencode Gene: ENSG00000067829.20_11
Transcript (Including UTRs)
   Position: hg19 chrX:153,051,223-153,059,830 Size: 8,608 Total Exon Count: 13 Strand: -
Coding Region
   Position: hg19 chrX:153,051,327-153,059,781 Size: 8,455 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:153,051,223-153,059,830)mRNA (may differ from genome)Protein (393 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: IDH3G_HUMAN
DESCRIPTION: RecName: Full=Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit gamma; AltName: Full=NAD(+)-specific ICDH subunit gamma; Flags: Precursor;
CATALYTIC ACTIVITY: Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
ENZYME REGULATION: Activated by increasing ADP/ATP ratios and by Ca(2+) (By similarity).
SUBUNIT: Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 (By similarity).
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 34.77 RPKM in Brain - Cerebellum
Total median expression: 1183.38 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -17.2049-0.351 Picture PostScript Text
3' UTR -20.70104-0.199 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019818 - IsoCit/isopropylmalate_DH_CS
IPR001804 - Isocitrate/isopropylmalate_DH
IPR004434 - Isocitrate_DH_NAD
IPR024084 - IsoPropMal-DH-like_dom

Pfam Domains:
PF00180 - Isocitrate/isopropylmalate dehydrogenase

SCOP Domains:
53659 - Isocitrate/Isopropylmalate dehydrogenase-like

ModBase Predicted Comparative 3D Structure on P51553
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0004449 isocitrate dehydrogenase (NAD+) activity
GO:0005524 ATP binding
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0046872 metal ion binding
GO:0051287 NAD binding

Biological Process:
GO:0005975 carbohydrate metabolic process
GO:0006099 tricarboxylic acid cycle
GO:0006102 isocitrate metabolic process
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix


-  Descriptions from all associated GenBank mRNAs
  BC001902 - Homo sapiens isocitrate dehydrogenase 3 (NAD+) gamma, mRNA (cDNA clone MGC:2102 IMAGE:3543839), complete cds.
U40272 - Human NAD+-specific isocitrate dehydrogenase gamma subunit precursor mRNA, nuclear gene encoding mitochondrial protein, complete cds.
U69269 - Human clone ID 212803 NAD (H)-specific isocitrate dehydrogenase gamma subunit mRNA, alternatively spliced, partial cds.
U69268 - Human clone ID 193225 NAD (H)-specific isocitrate dehydrogenase gamma subunit mRNA, alternatively spliced, partial cds.
BC000933 - Homo sapiens isocitrate dehydrogenase 3 (NAD+) gamma, mRNA (cDNA clone MGC:5393 IMAGE:3447003), complete cds.
Z68907 - H.sapiens mRNA for NAD (H)-specific isocitrate dehydrogenase gamma subunit precursor.
AB209206 - Homo sapiens mRNA for isocitrate dehydrogenase 3 (NAD+) gamma isoform b precursor variant protein.
JD382969 - Sequence 363993 from Patent EP1572962.
JD386584 - Sequence 367608 from Patent EP1572962.
JD391245 - Sequence 372269 from Patent EP1572962.
JD381373 - Sequence 362397 from Patent EP1572962.
JD490317 - Sequence 471341 from Patent EP1572962.
JD556834 - Sequence 537858 from Patent EP1572962.
JD540873 - Sequence 521897 from Patent EP1572962.
KJ897029 - Synthetic construct Homo sapiens clone ccsbBroadEn_06423 IDH3G gene, encodes complete protein.
KJ904486 - Synthetic construct Homo sapiens clone ccsbBroadEn_13880 IDH3G-like gene, encodes complete protein.
AB528154 - Synthetic construct DNA, clone: pF1KE0422, Homo sapiens IDH3G gene for isocitrate dehydrogenase 3 (NAD+) gamma, without stop codon, in Flexi system.
AM393133 - Synthetic construct Homo sapiens clone IMAGE:100002072 for hypothetical protein (IDH3G gene).
AM393247 - Synthetic construct Homo sapiens clone IMAGE:100002039 for hypothetical protein (IDH3G gene).
JD417116 - Sequence 398140 from Patent EP1572962.
CU674926 - Synthetic construct Homo sapiens gateway clone IMAGE:100017169 5' read IDH3G mRNA.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY66-398 - TCA cycle
PWY66-407 - superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle

Reactome (by CSHL, EBI, and GO)

Protein P51553 (Reactome details) participates in the following event(s):

R-HSA-1268022 TOMM40 complex translocates proteins from the cytosol to the mitochondrial intermembrane space
R-HSA-70967 isocitrate + NAD+ => alpha-ketoglutarate + CO2 + NADH + H+ [IDH3]
R-HSA-1268020 Mitochondrial protein import
R-HSA-71403 Citric acid cycle (TCA cycle)
R-HSA-392499 Metabolism of proteins
R-HSA-71406 Pyruvate metabolism and Citric Acid (TCA) cycle
R-HSA-1428517 The citric acid (TCA) cycle and respiratory electron transport
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: E9PDD5, ENST00000217901.1, ENST00000217901.2, ENST00000217901.3, ENST00000217901.4, ENST00000217901.5, ENST00000217901.6, ENST00000217901.7, ENST00000217901.8, ENST00000217901.9, IDH3G_HUMAN, NM_004135, P51553, Q9BUU5, uc317cuj.1, uc317cuj.2
UCSC ID: ENST00000217901.10_7
RefSeq Accession: NM_004135.4
Protein: P51553 (aka IDH3G_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.