Human Gene IQGAP2 (ENST00000274364.11_5) from GENCODE V47lift37
  Description: IQ motif containing GTPase activating protein 2, transcript variant 1 (from RefSeq NM_006633.5)
Gencode Transcript: ENST00000274364.11_5
Gencode Gene: ENSG00000145703.17_15
Transcript (Including UTRs)
   Position: hg19 chr5:75,699,110-76,003,957 Size: 304,848 Total Exon Count: 36 Strand: +
Coding Region
   Position: hg19 chr5:75,699,371-76,003,138 Size: 303,768 Coding Exon Count: 36 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:75,699,110-76,003,957)mRNA (may differ from genome)Protein (1575 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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MGIOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: IQGA2_HUMAN
DESCRIPTION: RecName: Full=Ras GTPase-activating-like protein IQGAP2;
FUNCTION: Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
TISSUE SPECIFICITY: Isoform 2 expression is enhanced in testis.
SIMILARITY: Contains 1 CH (calponin-homology) domain.
SIMILARITY: Contains 3 IQ domains.
SIMILARITY: Contains 1 Ras-GAP domain.
SIMILARITY: Contains 1 WW domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 19.64 RPKM in Testis
Total median expression: 181.66 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -131.60261-0.504 Picture PostScript Text
3' UTR -152.00819-0.186 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001715 - CH-domain
IPR000048 - IQ_motif_EF-hand-BS
IPR001936 - RasGAP
IPR000593 - RasGAP_C
IPR023152 - RasGAP_CS
IPR008936 - Rho_GTPase_activation_prot
IPR001202 - WW_Rsp5_WWP

Pfam Domains:
PF00307 - Calponin homology (CH) domain
PF00612 - IQ calmodulin-binding motif
PF00616 - GTPase-activator protein for Ras-like GTPase
PF03836 - RasGAP C-terminus

SCOP Domains:
48350 - GTPase activation domain, GAP
47576 - Calponin-homology domain, CH-domain
51045 - WW domain
52540 - P-loop containing nucleoside triphosphate hydrolases
143885 - RGC domain-like
54980 - EF-G C-terminal domain-like
55486 - Metalloproteases ("zincins"), catalytic domain
58042 - Outer membrane lipoprotein

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3IEZ - X-ray MuPIT 4EZA - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q13576
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
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 RGD  WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003779 actin binding
GO:0005095 GTPase inhibitor activity
GO:0005516 calmodulin binding
GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
GO:0017048 Rho GTPase binding
GO:0048365 Rac GTPase binding
GO:0051015 actin filament binding
GO:0071933 Arp2/3 complex binding

Biological Process:
GO:0007165 signal transduction
GO:0034260 negative regulation of GTPase activity
GO:0034314 Arp2/3 complex-mediated actin nucleation
GO:0043087 regulation of GTPase activity
GO:0043312 neutrophil degranulation
GO:0070493 thrombin-activated receptor signaling pathway

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005874 microtubule
GO:0005886 plasma membrane
GO:0005902 microvillus
GO:0009986 cell surface
GO:0015629 actin cytoskeleton
GO:0030027 lamellipodium
GO:0030175 filopodium
GO:0030667 secretory granule membrane
GO:0070062 extracellular exosome
GO:0031941 filamentous actin


-  Descriptions from all associated GenBank mRNAs
  AK291066 - Homo sapiens cDNA FLJ76812 complete cds, highly similar to Homo sapiens IQ motif containing GTPase activating protein 2 (IQGAP2), mRNA.
U51903 - Human RasGAP-related protein (IQGAP2) mRNA, complete cds.
AK307555 - Homo sapiens cDNA, FLJ97503.
AK302510 - Homo sapiens cDNA FLJ51769 partial cds, highly similar to Ras GTPase-activating-like protein IQGAP2.
JD186567 - Sequence 167591 from Patent EP1572962.
JD180002 - Sequence 161026 from Patent EP1572962.
JD443225 - Sequence 424249 from Patent EP1572962.
JD336013 - Sequence 317037 from Patent EP1572962.
AB208856 - Homo sapiens mRNA for IQ motif containing GTPase activating protein 2 variant protein.
AK302599 - Homo sapiens cDNA FLJ52367 complete cds, highly similar to Ras GTPase-activating-like protein IQGAP2.
AY351902 - Homo sapiens testis specific IQ motif containing GTPase activating protein 2 (IQGAP2) mRNA, complete cds.
AK303054 - Homo sapiens cDNA FLJ50398 complete cds, highly similar to Ras GTPase-activating-like protein IQGAP2.
AK311619 - Homo sapiens cDNA, FLJ18661.
AK302981 - Homo sapiens cDNA FLJ52376 complete cds, highly similar to Ras GTPase-activating-like protein IQGAP2.
AK307626 - Homo sapiens cDNA, FLJ97574.
JD539072 - Sequence 520096 from Patent EP1572962.
JD311483 - Sequence 292507 from Patent EP1572962.
JD504786 - Sequence 485810 from Patent EP1572962.
JD089821 - Sequence 70845 from Patent EP1572962.
JD173891 - Sequence 154915 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q13576 (Reactome details) participates in the following event(s):

R-HSA-6798743 Exocytosis of secretory granule membrane proteins
R-HSA-5626469 GTP-bound RAC1 and CDC42 bind IQGAPs
R-HSA-5626507 IQGAPs bind F-actin, which is inhibited by calmodulin
R-HSA-5626549 IQGAPs bind CALM1
R-HSA-6798695 Neutrophil degranulation
R-HSA-5626467 RHO GTPases activate IQGAPs
R-HSA-168249 Innate Immune System
R-HSA-195258 RHO GTPase Effectors
R-HSA-168256 Immune System
R-HSA-194315 Signaling by Rho GTPases
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A8K4V1, B7Z8A4, ENST00000274364.1, ENST00000274364.10, ENST00000274364.2, ENST00000274364.3, ENST00000274364.4, ENST00000274364.5, ENST00000274364.6, ENST00000274364.7, ENST00000274364.8, ENST00000274364.9, IQGA2_HUMAN, J3KR91, NM_006633, Q13576, uc317jeu.1, uc317jeu.2
UCSC ID: ENST00000274364.11_5
RefSeq Accession: NM_006633.5
Protein: Q13576 (aka IQGA2_HUMAN or IQG2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.